A5058051428- Occupational and environmental lung diseases
- Lung Cancer Treatments and Mutations
- Lung Cancer Research Studies
- Cancer Research and Treatments
- Cancer Immunotherapy and Biomarkers
- Neuroendocrine Tumor Research Advances
- Melanoma and MAPK Pathways
- CAR-T cell therapy research
- Peptidase Inhibition and Analysis
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Multiple Myeloma Research and Treatments
- Ferroptosis and cancer prognosis
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Model-Driven Software Engineering Techniques
- Cancer-related Molecular Pathways
- Medical Imaging and Pathology Studies
- Neuroblastoma Research and Treatments
- Pleural and Pulmonary Diseases
- Lung Cancer Diagnosis and Treatment
- Educational Games and Gamification
- Molecular Biology Techniques and Applications
- Lymphoma Diagnosis and Treatment
- Tracheal and airway disorders
- Breast Cancer Treatment Studies
University of Duisburg-Essen
2014-2023
Ruhrlandklinik
2013-2022
Essen University Hospital
1953-2021
Deutschen Konsortium für Translationale Krebsforschung
2019
West Cancer Center
2019
The Christie NHS Foundation Trust
2018
Louisiana State University in Shreveport
2017
National Institute of Pathology
2016
CollegeAmerica
2014
Springer Nature (United States)
2014
To evaluate the histopathologic changes influencing Doppler measurements of resistive index (RI) in renal arteries parenchymal diseases, 68 kidneys 34 consecutive patients with various forms diseases were studied by duplex ultrasound (duplex US) immediately before percutaneous biopsy. The RI, length, and cortical echogenicity correlated amount glomerular, interstitial, vascular graded on a scale from 0 to 100. resistance therefore RI are significantly prevalence arteriolosclerosis,...
Lung cancer is the leading cause of cancer-related deaths worldwide. The typical and atypical carcinoid (TC AC), large-cell neuroendocrine carcinoma (LCNEC) small-cell lung cancers (SCLC) are subgroups pulmonary tumours that show differentiations. With rising impact molecular pathology in routine diagnostics interest for reliable biomarkers, which can help to differentiate these may enable a more personalised treatment patients, grows. A collective 70 formalin-fixed, paraffin-embedded (FFPE)...
Background Neuroendocrine lung cancer (NELC) represents 25% of all cases and large patient collectives exist as formalin-fixed, paraffin-embedded (FFPE) tissue only. FFPE is controversially discussed source for molecular biological analyses reference genes NELC are poorly establishes. Material methods Forty-three representative FFPE-specimens were used mRNA expression analysis using the digital nCounter technology (NanoString). Based on recent literature, a total 91 targets investigated...
Background Lung cancer is the major cause of cancer-related deaths worldwide. Differential diagnosis can be difficult, especially when only small samples are available. Epigenetic changes frequently tissue-specific events in carcinogenesis and hence may serve as diagnostic biomarkers. Material methods 138 representative formalin-fixed, paraffin-embedded (FFPE) tissues (116 lung cases 22 benign controls) were used for targeted DNA methylation analysis via pyrosequencing ten literature-derived...
Malignant pleural mesothelioma (MPM) is a tumour arising from cavities with poor prognosis. Multimodality treatment pemetrexed combined cisplatin shows unsatisfying response-rates of 40%. The reasons for the rather efficacy chemotherapeutic are largely unknown. However, it conceivable that DNA repair mechanisms lead to an impaired therapy response. We hypothesize major role homologous recombination (HR) genome stability and survival this tumour. Therefore, we analysed genes compiled under...
Background: Neuroendocrine tumors of the lung (NELC) account for 25% all cancer cases and transcription factors may drive dedifferentiation these tumors. This study was conducted to identify supportive diagnostic prognostic biomarkers. Materials & methods: A total 16 TC, 13 AC, large cell neuroendocrine carcinomas 15 small were investigated mRNA expression 11 related genes (MYB, MYBBP1A, OCT4, PAX6, PCDHB, RBP1, SDCBP, SOX2, SOX4, SOX11, TEAD2). Results: SOX4 (p = 0.0002), SOX11 < 0.0001)...
Proteasomal subunit PSMB4 was suggested to be a survival gene in an animal model of hepatocellular carcinoma and glioblastoma cell lines. In pulmonary adenocarcinoma, high expression these genes found associated with poor differentiation survival. This study investigates the levels 26S proteasome subunits human neuroendocrine tumours including typical (TC) atypical (AC) carcinoid as well small (SCLC) large (LCNEC) carcinomas.Gene proteasomal (PSMA1, PSMA5, PSMB4, PSMB5 PSMD1) were...
Malignant pleural mesothelioma (MPM) is a highly aggressive tumour that first-line treated with combination of cisplatin and pemetrexed. Until now, predictive prognostic biomarkers are lacking, making it non-tailored therapy regimen unknown outcome. P53 frequently inactivated in MPM, but mutations extremely rare. MDM2 P14/ARF upstream regulators may contribute to inactivation.A total 72 MPM patients were investigated. immunoexpression was assessed 65 patients. mRNA expression analysed 48 the...
Immunotherapy targeting programmed cell death protein 1/programmed death-ligand 1 (PD-1/PD-L1) is a standard of care in the treatment stage IV non-small-cell lung cancer (NSCLC). However, only minority patients responds to anti-PD-1/PD-L1 monotherapy. Tumor-centric predictive biomarkers applicable small diagnostic specimens such as PD-L1 expression and tumor mutational burden [1.Prelaj A. Tay R. Ferrara et al.Predictive response for immune checkpoint inhibitors non–small-cell cancer.Eur J...
Aims: TP53 mutations are extremely rare in malignant pleural mesothelioma (MPM). In wild-type tumors, the functional p53 protein can be inactivated by MDM2. Materials & methods: A total of 61 patient samples were tested for their Mdm2 and expression levels via immunohistochemistry. Results: This study demonstrates nuclear three out four cell lines 21.3% MPM specimens investigated. After silencing MDM2 gene siRNA lines, immunoexpression is lost cells show changes indicative severe damage....
Background: Lung cancer still remains the leading cause of for men after prostate and breast women.Angiogenesis is considered a major microenvironment modifier.Material Methods: Demographic data study design; The based on collective twenty representative specimens each tumour entity (Typical Carcinoid, Atypical Large-Cell Neuroendocrine Carcinoma , Small Cell Cancer) mRNA expression analysis.The following methods were performed: RNA Extraction Integrity Assessment, NanoString CodeSet Design...
Purpose: Response to platinum-based therapy is a major prognostic factor in high-grade serous ovarian cancer (HGSOC). While the exact mechanisms of platinum-resistance remain unclear, evidence accumulating for connection between organism's immune-response and response platinum. However, predictive tools are missing. This study was performed examine putative role genetic tumor immune-microenvironment mediating differential chemotherapy HGSOC patients. Patients methods: Expression profiling...
Abstract Malignant pleural mesothelioma (MPM) is a rare, but aggressive tumor with dismal prognosis. Platinum-based chemotherapy regularly used as part of multimodality therapy. The expression metallothioneins (MT) has been identified reason for cisplatin resistance, which often leads to early therapy failure or relapse. Thus, knockdown MT may improve response treatment. gene- and protein the MPM-cell lines MSTO-211H, NCI-H2052 NCI-H2452 human fibroblast cell line MRC-5, well their...
The exact mechanism of desmoplastic stromal reaction (DSR) formation is still unclear. interaction between cancer cells and cancer-associated fibroblasts (CAFs) has an important role in tumor progression, while changes are a poor prognostic factor pleural mesothelioma (PM). We aimed to assess the impact CAFs paracrine signaling within microenvironment DSR presence on survival, cohort 77 PM patients. was evaluated morphologically by immunohistochemistry for Fibroblast activation protein alpha...
We propose a course design that fits practical game development project into regular lecture course. As this approach requires consistent structure, our concept proposes an adaption of Scrum is based on the life-cycle: ABC-Sprints consist three Sprints to iteratively create Alpha, Beta and Completed versions game. present detailed walkthrough give results formal evaluation. These indicate help students manage their workload increase productivity over time. Consequently, teams each presented...
Medullary thyroid carcinoma (MTC) occurs sporadically in 75% of patients. Metastatic disease is associated with significantly poorer survival. The aim this study was to identify prognostic markers for progressive MTC and oncogenic factors response vandetanib therapy.Clinical courses 32 patients sporadic (n=10 pN0cM0, n=8 pN1cM0, n=14 pN1cM1) were compared genetic profiles the patients' primary tumour tissue. Analysis RET proto-oncogene mutations performed by Sanger sequencing next-generation...
Malignant pleural mesothelioma (MPM) is a rare, predominantly asbestos-related and biologically highly aggressive tumour leading to dismal prognosis. Multimodality therapy consisting of platinum-based chemotherapy the treatment choice. The reasons for rather poor efficacy platinum compounds remain largely unknown.For this exploratory mRNA study, 24 FFPE specimens were screened by digital gene expression analysis. Based on data from preliminary experiments recent literature, total 366 mRNAs...
Neuroendocrine tumors of the lung comprise typical (TC) and atypical carcinoids (AC), large-cell neuroendocrine cancer (LCNEC) small-cell (SCLC). Cell cycle apoptosis are key pathways multicellular homeostasis deregulation these is associated with cancerogenesis.Sixty representative FFPE-specimens (16 TC, 13 AC, 16 LCNEC 15 SCLC) were used for mRNA expression analysis using NanoString technique. Eight genes related to ten regulating points cell investigated.ASCL1, BCL2, CASP8, CCNE1, CDK1,...
// Robert Fred Henry Walter 1, 2 , Claudia Vollbrecht 3, 4 Werner Jeremias Wohlschlaeger Daniel Christian Christoph 5 Kurt Schmid Fabian Dominik Mairinger 2, 1 Ruhrlandklinik, West German Lung Centre, University Hospital Essen, of Duisburg-Essen, Germany Institute Pathology, 3 Cologne, Köln, Division Molecular Charité, Berlin, Department Medical Oncology, Cancer Correspondence to: Mairinger, e-mail: fabian_mairinger@hotmail.com Keywords: MDM2, microRNA, pleural mesothelioma,...