A5000235243- RNA Research and Splicing
- MicroRNA in disease regulation
- Circular RNAs in diseases
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Chemical Synthesis and Analysis
- Analytical Chemistry and Chromatography
- RNA Interference and Gene Delivery
- RNA regulation and disease
- Liver physiology and pathology
- Metal-Catalyzed Oxygenation Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Bioinformatics and Genomic Networks
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Gene expression and cancer classification
- Protease and Inhibitor Mechanisms
- Cancer-related gene regulation
- Glioma Diagnosis and Treatment
- Hedgehog Signaling Pathway Studies
- Cancer-related molecular mechanisms research
- Cancer Cells and Metastasis
- Advanced biosensing and bioanalysis techniques
- Melanoma and MAPK Pathways
- Cancer Treatment and Pharmacology
German Cancer Research Center
2014-2021
Heidelberg University
2017-2021
Cancer Research UK
2021
Cancer Research UK Scotland Institute
2021
Center for Genomic Science
2016
Italian Institute of Technology
2016
DKFZ-ZMBH Alliance
2014
The regulation of miRNAs is critical to the definition cell identity and behavior in normal physiology disease. To date, dynamics miRNA degradation mechanisms involved remain largely obscure, particular, higher organisms. Here, we developed a pulse-chase approach based on metabolic RNA labeling calculate decay rates at genome-wide scale mammalian cells. Our analysis revealed heterogeneous half-lives, with many species behaving as stable molecules ( T 1/2 > 24 h), while others, including...
Protein synthesis is an essential process, deregulated in multiple tumor types showing differential dependence on translation factors compared to untransformed tissue. We show that colorectal cancer (CRC) with loss-of-function mutation the APC suppressor depends oncogenic program regulated by ability sense phosphorylated eIF2α (p-eIF2α). Despite increased protein rates following loss, phosphorylation, typically associated inhibition, enhanced CRC. Elevated p-eIF2α, and its proper sensing...
Abstract Background Regulation of protein output at the level translation allows for a rapid adaptation to dynamic changes cell’s requirements. This precise control gene expression is achieved by complex and interlinked biochemical processes that modulate both synthesis rate stability each individual mRNA. A major factor coordinating this regulation Ccr4-Not complex. Despite playing role in most stages mRNA life cycle, no attempt has been made take global integrated view how affects...
Breast cancer is the most common in women worldwide. The tumor microenvironment contributes to progression by inducing cell dissemination from primary and metastasis. TGF β signaling involved breast specifically elevated during metastatic transformation aggressive cancer. In this study, we performed genomewide correlation analysis of TGFBR 2 expression a panel 51 lines identified that MET coregulated with . This was confirmed at protein level human tissues. Flow cytometric luminal basal‐like...
Protein responses to extracellular cues are governed by gene transcription, mRNA degradation and translation, protein degradation. In order understand how these time-dependent processes cooperate generate dynamic responses, we analyzed the response of human mammary cells epidermal growth factor (EGF). Integrating transcript data into a mathematical model, inferred for several proteins their pre-and post-stimulus translation coefficients found that they exhibit complex, variation....
Triple negative breast cancer (TNBC) is the most aggressive subtype of (BC). Treatment options for TNBC patients are limited and further insights into disease aetiology needed to develop better therapeutic approaches. microRNAs' ability regulate multiple targets could hold a promising discovery approach pathways relevant aggressiveness. Thus, we address role miRNAs in controlling three signalling biology TNBC, their downstream phenotypes.To identify regulating WNT/β-catenin, c-Met, integrin...
Abstract Background Regulation of protein output at the level translation allows for a rapid adaptation to dynamic changes cell’s requirements. This precise control gene expression is achieved by complex and interlinked biochemical processes that modulate both synthesis rate stability each individual mRNA. A major factor coordinating this regulation Ccr4-Not complex. Despite playing role in most stages mRNA life cycle, no attempt has been made take global integrated view how affects...
Abstract microRNAs (miRNAs) sind kleine, nicht für Proteine codierende RNAs, die wichtige biologische Prozesse regulieren. Neben Funktionen in der natürlichen Entwicklung von Lebewesen auch Beteiligungen an Kranksheitsprozessen wie Krebs beschrieben worden. Diese Regulation erfolgt durch Basenpaarung mit mRNAs Zielgenen, und führt entweder zu deren Abbau oder einer Blockade Herstellung diesen codierten Proteinen. Da innerhalb miRNAs nur eine sehr kurze, etwa 6‐7 Nukleotide lange...
ABSTRACT Background Triple Negative Breast Cancer (TNBC) is the most aggressive subtype of (BC). Treatment options for TNBC patients are limited and further insights into disease aetiology needed to develop better therapeutic approaches. microRNAs’ ability regulate multiple targets could hold a promising discovery approach pathways relevant aggressiveness. Thus, we address role miRNAs in controlling signalling phenotypes biology TNBC. Methods To identify regulating WNT/β-catenin, c-Met,...