A5100706064- Parasitic Infections and Diagnostics
- Heme Oxygenase-1 and Carbon Monoxide
- Legionella and Acanthamoeba research
- Ginseng Biological Effects and Applications
- Advanced biosensing and bioanalysis techniques
- Bacteriophages and microbial interactions
- MXene and MAX Phase Materials
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Quinazolinone synthesis and applications
- Ocular Infections and Treatments
- Vector-Borne Animal Diseases
- Parasites and Host Interactions
- Viral gastroenteritis research and epidemiology
- Biochemical and Molecular Research
- Viral Infections and Vectors
- Animal Disease Management and Epidemiology
- Zoonotic diseases and public health
- Metal-Organic Frameworks: Synthesis and Applications
- Neuroscience and Neuropharmacology Research
- Functional Brain Connectivity Studies
- Dengue and Mosquito Control Research
- Antimicrobial Resistance in Staphylococcus
- Viral Infections and Outbreaks Research
- Cancer Treatment and Pharmacology
Jilin Medical University
2015-2025
Yanbian University
2024
Health Commission of Jilin Province
2017-2020
Kunming Institute of Zoology
2014
Chinese Academy of Sciences
2014
Jilin City Central Hospital
2014
Capital Medical University
2008
Schematic illustration of (a) the preparation DHA@ZIF-8 NPs and (b) their application for cancer therapy.
Lymphocytic choriomeningitis virus (LCMV) is a rodent-borne arenavirus that considered neglected cause of neurologic diseases in humans. In this study, we described genomic characterization newly isolated LCMVs Haemaphysalis longicornis, Dermacentor nuttalli, silvarum and Ixodes persulcatus Jilin Province, northeastern China. The complete sequences the small (S) large (L) segments ticks contained 3,375 7,235–7,241 nucleotides, respectively. Sequence comparison showed 82.1%–86.0% identity S...
Redox-responsive drug delivery system emerges as a hopeful platform for tumor treatment. Dihydroartemisinin (DHA) has been investigated an innovative therapeutic agent. Herein, DHA dimeric prodrug bridged with disulfide bond linker (DHA2-SS) designed and synthesized. The prepared prodrugs could self-assemble into nanoparticles (SS NPs) high content (> 90%) robust stability. These SS NPs display sensitive redox responsive capability can release under the heterogeneity microenvironment....
Dihydroartemisinin (DHA), a plant-derived natural product, has recently been proven to be an effective therapeutic agent for cancer treatment. Nevertheless, the poor water solubility and low bioavailability of DHA seriously impede its clinical applications. Herein, simple green strategy based on self-assembly was developed synthesize carrier-free nanoparticles (NPs). The resulting nanodrug (DHA NPs) formed by molecules via hydrogen bonding hydrophobic interactions. NPs exhibited...
Acanthamoeba species are ubiquitous, free-living protozoa that can invade the cornea and result in keratitis (AK), a painful progressive sight-threatening corneal disease. Disease progression current animal models is too rapid to mimic AK humans accurately. This study provides novel method for establishing rabbits compared it with conventional regard pathogenesis immune response humans. The New Zealand white were randomly divided into two experimental groups (Groups A B). Rabbits Group (n =...
Na-ASP-2 is a major protein secreted by infective third-stage larvae (L3) of the human hookworm Necator americanus upon host entry. It was chosen as lead vaccine candidate for its ability to elicit protective immune responses. However, clinical development this antigen recombinant halted because it caused allergic reactions among some volunteers previously infected with N. americanus. To prevent IgE-mediated induced but keep immunogenicity antigen, we designed and tested genetically...
Ficolin-2 (FCN2) is an innate immune pattern recognition molecule that can activate the complement pathway, opsonophagocytosis, and elimination of pathogens. The present study aimed to investigate association FCN2 gene single nucleotide polymorphisms (SNPs) with susceptibility pulmonary tuberculosis (TB). A total seven SNPs in exon 8 (+6359 C>T +6424 G>T) promoter region (-986 G>A, -602 -557 A>G, -64 A>C -4 A>G) were genotyped using PCR amplification DNA sequencing methods healthy controls...
Background Giardia lamblia is one of the most common infectious protozoans in human that may cause diarrhea travelers. Searching for antigens induced effectively protective immunity has become a key point development vaccine against giardiasis. Methodology/Principal Findings Mice vaccinated with G. trophozozite-specific α1-giardin DNA delivered orally by attenuated Salmonella typhimurium SL7027 elicited 74.2% trophozoite reduction, but only 28% reduction cyst shedding compared PBS buffer...
Dihydroartemisinin (DHA) has been regarded as a potential anticancer agent in recent years. Nevertheless, the clinical applications of DHA are seriously restricted result its intrinsic characteristics, such poor water solubility, instability, and fast clearance. Herein, type fluorescent nanoparticles was successfully fabricated via supramolecular assembling carbon dots (CDs) DHA. The formulated CDs-DHA not only significantly improve solubility stability DHA, but also possess favorable...
Giardia lamblia (G. lamblia) is the cause of giardiasis, a common infection that affects general population world. Despite constant possibility damage because their own metabolism, G. has survived and evolved to adapt various environments. However, research on energy-metabolism conversion in limited. This study aimed reveal dynamic metabolism mechanism under sugar starvation by detecting global lysine acetylation (Kac) 2-hydroxyisobutyrylation (Khib) sites combined with quantitative proteome...
Acanthamoeba castellanii is a pathogenic and opportunistic free-living amoeba that causes keratitis (AK) granulomatous amebic encephalitis (GAE) in immunocompromised individuals. The biological characterizations behind this protozoan not fully understood. This study aimed to determine the functions of heat shock protein (HSP)-20 A. ( Ac -HSP20) involved maintenance life cycle infectivity . Immunoscreening cDNA library with infected rabbit sera identified three positive clones, one them was...
We investigated Alongshan virus infection in reindeer northeastern China. found that 4.8% of the animals were viral RNA-positive, 33.3% tested positive for IgG, and 19.1% displayed neutralizing antibodies. These findings suggest could serve as sentinel animal species epidemiologic surveillance infection.
Microwaves may exert adverse biological effects on the cardiovascular system at integrated and cellular levels. However, mechanism underlying such remains poorly understood. Here, we report a previously uncharacterized through which microwaves damage myocardial cells. Rats were treated with 2450 MHz microwave radiation 50, 100, 150, or 200 mW/cm(2) for 6 min. Microwave treatment significantly enhanced levels of various enzymes in serum. In addition, it increased malondialdehyde content while...
Ginsenoside F2 and cyanidin-3-<italic>O</italic>-glucoside synergistically inhibited H<sub>2</sub>O<sub>2</sub>-induced apoptosis in HEK-293 cells through mitochondria-mediated apoptotic NF-κB pathways.
To investigate the inhibitory mechanisms of ginsenoside F1 on hydrogen peroxide induced cholesterol metabolism disorder and oxidative stress in HepG2 cells.
An artificial semisynthetic material can be derived from artemisinin (ART) called dihydroartemisinin (DHA). Although DHA has enhanced antigiardial potential, its clinical application is limited because of poor selectivity and low solubility. The drug’s absorption a direct impact on the cell, mechanism research to destruction cytoskeleton. In this study, we used zeolitic imidazolate framework-8 loaded it with (DHA@Zif-8) improve potential. DHA@Zif-8 enhance cellular uptake, increase...
Abstract Cyanidin‐3‐O‐glucoside (C3G), as a typical anthocyanin, exhibits excellent antioxidant effects. This study aimed to demonstrate the role and mechanism of C3G in regulating 3‐hydroxy‐3‐methylglutaryl‐coenzyme A reductase (HMGCR)‐mediated cholesterol anabolism on H 2 O ‐induced oxidative stress HEK‐293T cells. Firstly, inhibitory effect was confirmed by CCK‐8, ROS, mitochondrial membrane potential (MMP) experiments. Then, proteomics used investigate screen differentially expressed...
The encystation of Acanthamoeba castellanii has important ecological and medical significance. Blocking is the key to preventing transmission curing infections caused by A. castellanii. formation autophagosomes one most changes that occur during Acanthamoeba. Our previous studies have shown heat shock protein 20 (Ac-HSP20) involved in its encystation. This study aimed determine role mechanism Ac-HSP20 regulating autophagy
This study describes the purification and preparation of tricin (5, 7, 4-trihydroxy-3, 5-dimethoxyflavone) from Carex Meyeriana Kunth via adsorption desorption using macroporous resins high-performance liquid chromatography. Six were tested to evaluate static capacities. The HPD-300 resin was selected as material enrich because its suitable Adsorption thermodynamics kinetics studied on resin, results agreed with Langmuir model quasi-second-order model, respectively. parameters dynamic tests...
To construct rapidly a full-length cDNA library from nanogram amounts total RNA of Giardia lamblia (G. lamblia) trophozoites stocked in stabilization reagent.Total was extracted using Trizol reagent. A G. constructed by long-distance PCR (LD-PCR) method. The recombinant rate and the coverage clones were evaluated. inserted fragments identified sequenced amplification.The titer 3.85 × 10(7) pfu/mL. length ranged 0.4 to 2.5 kb, recombination efficiency accounted for 100% (20/20). is high...