Large-scale outbreaks of virus-associated severe diarrhea have occurred in pig populations since 2010. To investigate the prevalence and genetic evolution diarrhea-associated viruses responsible for outbreaks, we tested 1,791 samples collected from 213 farms five provinces southern China between 2021 2023. The test results showed that porcine epidemic virus (PEDV) was most frequently detected virus. rates ranged 47.40 to 52.22% 76.06% (162/213) farms. Porcine rotavirus (PoRV) second common...

In China, large-scale outbreaks of severe diarrhea caused by viruses have occurred in pigs since late 2010. To investigate the prevalence and genetic evolution diarrhea-associated responsible for outbreaks, a total 2987 field diarrheal samples collected from 168 pig farms five provinces Southern China during 2012-2018 were tested.Porcine epidemic virus (PEDV) was most frequently detected with rates between 50.21 62.10% samples, 96.43% (162/168) premises, respectively. Porcine...

Abstract Porcine epidemic diarrhea virus (PEDV) is a devastating cause of in pigs worldwide. Most studies have focused on molecular and pathogenic characterization PEDV, whereas there were limited understanding the role gut microbiota (GM) viral-associated diarrhea. Here, using Illumina MiSeq platform, we examined compared impact PEDV infection GM sows their piglets less than 10 days old. Our results showed that caused alternations structure abundance from levels phylum to genus, even...

Here, we examined the effects of crossbreeding and sex on growth performance, slaughter meat quality in Xingguo gray (XG) goose, using transcriptomic metabolomic techniques. The experiment was conducted 400 goslings (1-day old) 2 genotypes: XG breed its ternary hybrids [F2 geese; (XG Goose♂ × Yangzhou Goose♀)♀ Shitou Goose♂]. were divided into 4 groups: female XG, male F2 geese, parameters at 70 d age, 30 birds from each group. Following slaughter, samples breast thigh muscles collected...

Avian leukosis virus subgroup K (ALV-K) is a new of avian (ALV) that was first identified in Chinese native chickens recent years. To further understand the molecular epidemiology and evolutionary diversity ALV-K, this study investigated 73,664 chicken plasma samples collected from Jiangxi flocks. The results showed ALV-J most predominant ALV subtype chickens, with high positivity rate 4.34%. From 2021 to 2023, there gradual upward trend proportion positive numbers ALV-K among ALV-positive...

Getah virus (GETV) is a mosquito-borne with broad host range, including mosquitoes and various animals such as pigs, horses, goats, cattle, boars, blue foxes. In GETV can cause fever, abortion reproductive disorders in sows, well tremors, diarrhea piglets, posing serious threat to the pig production industry. However, there are few reports on epidemiology of China, reliable diagnostic kits for large-scale detection antibodies lacking. Therefore, this study aimed establish rapid, sensitive...

The nonstructural protein 13 (NSP13) of PDCoV is a highly conservative helicase and plays key roles in viral replication. NSP13 contains zinc-binding domain (ZBD), helical Stalk domain, beta-barrel 1B core domain. However, the specific functions these domains remain largely unknown. Here, we expressed purified wild-type NSP13WT various mutants with deletions, activities proteins were analyzed using multiple methods. We found that NSP13ΔZBD possessed abilities to hydrolyze ATP unwind...

Getah virus (GETV) is a mosquito-borne Alphavirus that causes fever, rash and oedema in horses, anorexia neurological symptoms as well piglet diarrhea deaths reproductive disorders sows blue foxes, the endemic areas are expanding progressively, especially Asia Oceania. In order to study current epidemiological status of GETV wild boar herds its genetic evolutionary patterns, we conducted survey genomic analyses pig herds. The results showed 74.02% (57/77) samples tested ( n = 77) were...

The full-length genome sequence of a novel swine acute diarrhea syndrome coronavirus (SADS-CoV), CH/FJWT/2018, was determined, which genetically most closely related to CN/GDWT/2017, recently discovered in Fujian, China. indel sites the spike (S) gene CH/FJWT/2018 were similar those bat-origin SADS-related coronaviruses.

To date, two genotypes, i.e., genotype 1 (G1) and 2 (G2), of porcine epidemic diarrhea virus (PEDV) have been identified in swine, while the cross protection between G2a G1a subgenotypes is undetermined. Hence, present study, we attempted to observe a comparative pathogenicity (CV777) (CH/JX/01) PEDVs. Initially pregnant sows were vaccinated twice with kinds inactivated G1a- G2a-based PEDV vaccines, respectively delivered neonatal piglets challenged prototype isolates PEDVs, then...

Goose astrovirus (GoAstV) is a new Avastrovirus of the genus causing gout, hemorrhage, and swellings kidneys that have affected goslings around major goose-producing regions in China. The GoAstV divided into goose type 1 (GoAstV-1) 2 (GoAstV-2). Although GoAstV-2 known to be causative agent little published information about relationship between GoAstV-1 gout unknown. In this study, we investigated presence 293 visceral tissue/dead embryos samples with on different farms Jiangxi province, A...

Avian leukosis caused by avian virus (ALV) is one of the most severe diseases endangering poultry industry. When eradication measures performed in commercial broilers and layers have achieved excellent results, ALV some local chickens has gradually attracted attention. Since late 2018, following re-outbreak ALV-J white feather China, AL-like symptoms also suddenly broke out flocks, leading to great economic losses. In this study, a systematic epidemiological survey was carried eight chicken...

The epidemic of goose astrovirus (GoAstV) caused huge losses to the poultry industry. Epidemiological studies in China revealed 2 circulating genotypes GoAstV, but there is a lack differential diagnosis tools. By analyzing all published genomes this study designed specific PCR primers and Taqman probes recognize different GoAstV. After optimization verification, developed Taqman-based real-time quantitative method that capable diagnosis. established qPCR exhibited detection limitations 100...

Scavenger receptor class A, member 5 (SCARA5) is a of the scavenger family, and involved in several types human malignancy; however, its roles osteosarcoma (OS) remain to be fully elucidated. Therefore, present study, biological functions SCARA5 OS, potential underlying mechanisms were investigated. expression OS tissues cell lines was detected by reverse transcription‑quantitative polymerase chain reaction western blot analysis. The effects on proliferation migration/invasion ability cells...

Porcine Deltacoronavirus (PDCoV) is a newly emerged enteropathogenic coronavirus that causes diarrhea and mortality in neonatal piglets. PDCoV has spread to many countries around the world, leading significant economic losses pork industry. Therefore, rapid sensitive method for detection of clinical samples urgently needed. In this study, we developed single-tube one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay specific nucleocapsid gene diagnose monitor...

Since 2011, the Gyrovirus galga 1 (GyVg1, previously recognized as avian gyrovirus 2) strain has extensively been detected worldwide. However, because there are no up-to-date reports of examining distribution GyVg1 in flocks southern China, epidemiology this virus is unknown. To investigate prevalence and genetic evolution GyVg1, a total 2,077 field samples collected from 113 chicken farms 6 provinces China during 2020 to 2022 were tested. Among them, 315 (315/2,077, 15.17%) positive for by...

Abstract:

Porcine circovirus 3 (PCV3) infections have been reported in different clinical presentations. However, the prevalence and pathogenicity of PCV3 associated with diarrhea piglets limited. Herein, we present an investigation genome analyses experiencing diarrhea, observed signs, gross lesions, histological changes pigs negative for all known pathogens but positive alone. Among feces (n = 141) tested, 16.31% (23/141) were PCV3. Of which, 27.28% (15/55) 14.29% (5/35) diarrheal samples from...

ABSTRACT The whole-complete genome sequence of a strain duck tembusu virus (DTMUV), DTMUV/CH/2014, affecting layer ducks in China, was determined and characterized. Compared with DTMUV sequences available GenBank, DTMUV/CH/2014 has 3 amino acid mutations located the capsid, prM, NS3 genes DTMUV/CH/2014.

The new emergence of swine acute diarrhea syndrome coronavirus (SADS-CoV) has resulted in high mortality suckling pigs China. To date, the transcriptional expression host cells during SADS-CoV infection not been documented. In this study, by means RNA-Seq technology, we investigated whole genomic profiles intestinal porcine epithelial (IPEC-J2) infected with a strain SADS-CoV-CH-FJWT-2018. A total 24,676 genes were identified: 23,677 known genes, and 999 novel genes. 1,897 differentially...

Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen that causes severe infections in humans and the swine industry. Acquisition utilization of available carbon sources from challenging host environments necessary for bacterial pathogens to ensure growth proliferation. Glycogen abundant mammalian body may support SS2 during infection hosts. However, limited information known about mechanism between glycogen adaptation SS2. Here, pleiotropic effects exogenous on were...

Abstract Background Goose astrovirus (GoAstV) is an important pathogen that causes joint and visceral gout in goslings. It has been circulating many provinces of China since 2017. genotypes 2 (GoAstV-2) the main epidemic strain, its high morbidity mortality have caused huge economic losses to goose industry. An accurate point-of-care detection for GoAstV-2 great significance. In this study, we developed a real-time reverse transcription recombinase polymerase amplification (RT-RPA) method...