A5087949893- Genomics and Chromatin Dynamics
- Genomic variations and chromosomal abnormalities
- Genetics and Neurodevelopmental Disorders
- Congenital heart defects research
- Psychedelics and Drug Studies
- Fungal and yeast genetics research
- Neuroscience and Neuropharmacology Research
- Amphibian and Reptile Biology
- RNA Research and Splicing
- Biochemical Analysis and Sensing Techniques
- Congenital Heart Disease Studies
- Genomics and Rare Diseases
- Neurotransmitter Receptor Influence on Behavior
- RNA modifications and cancer
- Zebrafish Biomedical Research Applications
- Reproductive Biology and Fertility
- Essential Oils and Antimicrobial Activity
- Cancer Cells and Metastasis
- Cardiac Structural Anomalies and Repair
- Animal Behavior and Reproduction
- Renal and related cancers
- Microtubule and mitosis dynamics
- Cannabis and Cannabinoid Research
- Plant Gene Expression Analysis
- Cardiomyopathy and Myosin Studies
Universidade de Brasília
2015-2024
Instituto de Ciencias Tecnológicas
2013-2018
Newcastle University
2003-2010
Brazilian Agricultural Research Corporation
2006
University of Newcastle Australia
2000
Abstract Background Mesenchymal stromal cells (MSCs) are multipotent progenitor used in several cell therapies. MSCs characterized by the expression of CD73, CD90, and CD105 markers, absence CD34, CD45, CD11a, CD19, HLA-DR markers. CD90 is a glycoprotein present MSC membranes also adult cancer stem cells. The role remains unknown. Here, we sought to analyse that plays characteristic properties vitro expanded human MSCs. Methods We investigated function with regard morphology, proliferation...
The use of natural oils in topical pharmaceutical preparations has usually presented safe agents for the improvement human health. Based on research into immense potential wound management and healing, we aimed to validate products by studying ability essential oil Eugenia dysenterica DC leaves (oEd) stimulate vitro skin cell migration. Skin cytotoxicity was evaluated using a fibroblast line (L929) MTT assay. chemical profile investigated GC-MS. Moreover, inhibition lipopolysaccharide (LPS)...
The forkhead transcription factor Fkh2p acts in a DNA-bound complex with Mcm1p and the coactivator Ndd1p to regulate cell cycle-dependent expression of CLB2 gene cluster Saccharomyces cerevisiae. Here, we demonstrate that is target cyclin-dependent protein kinases phosphorylation promotes interactions between Ndd1p. These phosphorylation-dependent changes Fkh2p-Ndd1p play an important role cycle-regulated cluster. Our data therefore identify regulatory for cycle further our molecular...
Breast cancer is a complex heterogeneous disease and one of the leading causes death among women. In addressing need for treatments this life-threatening illness, we studied 3,4-dihydropyrimidin-2(1H)-one (or thione) derivatives (DHPMs), class inhibitor molecules Eg5 motor spindle protein that shows pronounced antitumor activity against several cell lines. An in vitro screening was performed identification DHPMs with potent effects on MCF-7 MDA-MB-231 cells selected were evaluated their...
Transcriptional control is exerted by the antagonistic activities of activator and repressor proteins. In Saccharomyces cerevisiae, transcription factor complexes containing MADS box protein Mcm1p are key regulators cell cycle-dependent at both G2/M M/G1 transitions. The homeodomain Yox1p acts in a complex with to timing gene expression. Here, we show that interacts through motif located N terminally its homeodomain. functions as transcriptional competing forkhead Fkh2p for binding...
The mouse, chicken, African clawed frog, and zebrafish are considered ¨model organisms¨ due to their extensive embryological genetic characterization. However, they far from representative of known diversity, impairing phylogenetic analyses developmental patterns. Since squamates have historically received limited attention in studies, we here describe the sequence for Tropidurus torquatus, provide first post-ovipositional series lizard family Tropiduridae. Fifteen stages described based on...
ABSTRACT In eukaryotes the regulation of gene expression plays a key role in controlling cell cycle progression. Here, we demonstrate that forkhead transcription factor, Fkh2, regulates periodic cdc15 + and spo12 M G 1 phases division fission yeast Schizosaccharomyces pombe . We also show Fkh2 is important for several processes, including morphology separation, nuclear structure migration, mitotic spindle function. find fkh2 itself regulated cycle-dependent manner coincident with cdc18 ,...
Ayahuasca is a psychoactive concoction prepared from the plants Banisteriopsis caapi and Psychotria viridis which are used ancestrally by Amazonian Indian populations more recently, Christian religious groups in Brazil other countries. The aims of present study were to identify effects ayahuasca on zebrafish embryo development neurobehavior. Toxicity developmental endpoints for embryos assessed 0 1000 mg/L over 96 h exposure. locomotor activity larvae using video tracking system (ZebraBox)...
Piplartine, also known as piperlongumine, is a natural and biologically active amide alkaloid found in various Piper species within the Piperaceae family. It possesses numerous beneficial properties that can be leveraged development of nanotechnological pharmaceutical products. However, information on effects piplartine mammalian embryonic scarce. This study aims to assess general toxicity teratogenic potential during mice. Pregnant mice received daily treatments 25, 50, or 100 mg/kg via...
Ayahuasca is a psychoactive beverage used ancestrally by indigenous Amazonian tribes and, more recently, Christian religions in Brazil and other countries. This study aimed to investigate the reproductive effects of this male Wistar rats after chronic exposure. The were treated gavage every day for 70 days at 0 (control), 1×, 2×, 4× 8× dose religious ritual (12 animals per group), euthanized on 71st day. Compared controls, there was significant decrease food consumption body weight gain from...
The yeast MADS-box transcription factor Mcm1p plays an important regulatory role in several diverse cellular processes. In common with a subset of other factors, elicits substantial DNA bending. However, the protein-induced bending by proteins eukaryotic gene regulation is not understood. Here, we demonstrate for Mcm1p-mediated determining local promoter architecture and permitting formation ternary complexes. We constructed mutant mcm1 alleles that are defective Defects nuclear division,...
Duplications of the long arm chromosome 1 are rare. Distal duplications most common and have been reported as either pure trisomy or unbalanced translocations. The paucity cases with distal 1q has made it difficult to delineate a partial syndrome. Here, we report 2 patients overlapping detected by G-banding. Array CGH FISH were performed characterize duplicated segments, exclude involvement other chromosomes determine orientation duplication. Patient presents mild phenotype carries 22.5-Mb...