A5087497455- Ear Surgery and Otitis Media
- Salivary Gland Tumors Diagnosis and Treatment
- Hearing, Cochlea, Tinnitus, Genetics
- Hearing Loss and Rehabilitation
- Ear and Head Tumors
- Head and Neck Cancer Studies
- Oral and Maxillofacial Pathology
- Vestibular and auditory disorders
- Nasal Surgery and Airway Studies
- Voice and Speech Disorders
- Facial Nerve Paralysis Treatment and Research
- Head and Neck Surgical Oncology
- Tracheal and airway disorders
- Sinusitis and nasal conditions
- Cancer Immunotherapy and Biomarkers
- Meningioma and schwannoma management
- Reconstructive Facial Surgery Techniques
- Pituitary Gland Disorders and Treatments
- Tumors and Oncological Cases
- Airway Management and Intubation Techniques
- Cancer Diagnosis and Treatment
- Hearing Impairment and Communication
- Vascular Malformations and Hemangiomas
- Immune Cell Function and Interaction
- Photoreceptor and optogenetics research
Universitätsmedizin Göttingen
2017-2025
University of Göttingen
2006-2025
Universitätsklinik für Hals-, Nasen- und Ohrenheilkunde
2020-2024
Klinik und Poliklinik für Hals-, Nasen-, Ohrenheilkunde
2021-2023
University of Cologne
2012-2022
Medizinische Hochschule Hannover
2022
Klinik und Poliklinik für Hals-, Nasen-, Ohrenheilkunde
2011-2021
University Hospital Cologne
2009-2021
HNO Bad Bramstedt
2019
HNO-Praxis
2019
Hearing in mammals relies on the highly synchronous synaptic transfer between cochlear inner hair cells (IHCs) and auditory nerve. We studied presynaptic function of single mouse IHCs by monitoring membrane capacitance changes voltage-gated Ca 2+ currents. Exocytosis initially occurred at a high rate but then slowed down within few milliseconds, despite nearly constant influx. interpret observed secretory depression as depletion readily releasable pool (RRP) about 280 vesicles. These...
Before mice start to hear at approximately postnatal day 10, their cochlear inner hair cells (IHCs) spontaneously generate Ca(2+) action potentials. Therefore, immature IHCs could stimulate the auditory pathway, provided that they were already competent for transmitter release. Here, we combined patch-clamp capacitance measurements and fluorimetric [Ca(2+)](i) recordings study presynaptic function of during maturation. Ca(2+)-dependent exocytosis subsequent endocytic membrane retrieval...
B lymphocytes are important players in immune responses to cancer. However, their composition and function head neck squamous cell carcinoma (HNSCC) has not been well described. Here, we analyzed subsets HNSCC (n = 38), non-cancerous mucosa 14) peripheral blood from patients 38) healthy controls 20) by flow cytometry. Intratumoral cells contained high percentages of activated (CD86+), antigen-presenting (CD86+/CD21−) memory (IgD−/CD27+). T follicular helper (CD4+/CXCR5+/CD45RA−/CCR7−) as key...
The composition of tumor-infiltrating lymphocytes (TIL) reflects biology and immunogenicity cancer. Here, we characterize T-cell subsets expression immune checkpoint molecules in head neck squamous cell carcinoma (HNSCC). We analyzed TIL primary tumors (n = 34), blood (peripheral mononuclear cells (PBMC); n 34) non-cancerous mucosa 7) 34 treatment-naïve HNSCC patients PBMC 15 healthy controls. Flow cytometry analyses revealed a highly variable infiltration mainly an effector memory phenotype...
Nowadays, several options are available to treat patients with conductive or mixed hearing loss. Whenever surgical intervention is not possible contra-indicated, and amplification by a conventional device (e.g., behind-the-ear device) feasible, then implantable devices an indispensable next option. Implantable bone-conduction middle-ear implants have advantages but also limitations concerning complexity/invasiveness of the surgery, medical complications, effectiveness. To counsel patient,...
Abstract Objectives/Hypothesis: To analyze the benefit of electromyographic (EMG) neuromonitoring during primary surgery on benign parotid lesions for postoperative facial function compared to visual observation only. Study Design: Prospective controlled clinical two‐center trial. Methods: Using an operation microscope, 100 parotidectomies in 96 patients were performed: 50 procedures with a continuous EMG monitoring plus (EMG group), and only control (control group). The rate weakness was...
Salivary gland cancer represents a heterogeneous group of malignant tumors. Due to their low incidence and the existence multiple morphologically defined subtypes, these tumors are still poorly understood with regard molecular pathogenesis therapeutically relevant genetic alterations.Performing systematic comprehensive study covering 13 subtypes salivary cancer, next generation sequencing was done on 84 tissue samples parotid using multiplex PCR for enrichment related gene loci hotspots 46...
Tumor-infiltrating lymphocytes (TILs) are correlated to prognosis of several kinds cancer. Most studies focused on T cells, while the role tumor-associated B cells (TABs) has only recently gained more attention. TABs contain subpopulations with distinct functions, potentially promoting or inhibiting immune responses. This study provides a detailed analysis in gastro-esophageal adenocarcinoma (EAC). Flow cytometric analyses single cell suspensions tumor samples, mucosa, lymph nodes and...
Head and neck squamous cell carcinoma (HNSCC) is a highly heterogeneous aggressive tumor originating from the epithelial lining of upper aero-digestive tract accounting for 300,000 annual deaths worldwide due to failure current therapies. The natural killer group 2D receptor (NKG2D) on (NK) cells several T subsets plays an important role immunosurveillance HNSCC thus targeted by immune evasion strategies in particular shedding various NKG2D ligands (NKG2DLs). Based plasma samples 44...
Background Interactions between tumor cells and in the microenvironment contribute to development metastasis. The spatial arrangement of individual relation each other influences likelihood whether how these interact with other. Methods This study investigated effect distribution on function leukocyte subsets human head neck squamous cell carcinoma (HNSCC) using multiplex immunohistochemistry (IHC). Leukocyte were further classified based analysis two previously published HNSCC single-cell...
Objective Otosclerosis leads to ossification of the stapes footplate and is responsible for a significant number patients having hearing loss. Stapes surgery treatment otosclerosis with excellent postoperative outcomes. The aim this retrospective study was analyze safety effectiveness titanium mAXIS prosthesis in multicentric setting. Methods Ninety-four surgeries implantation took place multicenter group. During follow-up at 59 (±48.1) days, pure-tone audiometry performed. averages 0.5, 1,...
<h3>Objective</h3> To assess the learning curve of sialendoscopy with modular endoscopes based on operative parameters and a postoperative performance rating. <h3>Design</h3> Prospective study, case series. <h3>Setting</h3> Tertiary referral hospital. <h3>Patients</h3> The study included 50 consecutive patients who underwent by single surgeon (J.C.L.) between September 2007 March 2009 at University Hospital Cologne, Germany. were chronologically arranged into 5 groups 10 patients....
Surgery can often eradicate chronic middle ear disease in patients with recurrent cholesteatoma, tubal dysfunction, and others; however, many cases, social hearing cannot be restored even after multiple revision tympanoplasties. A aid is then recommended. Placement of an implantable its advantage unoccluded canal, irrespective function, seems to a promising alternative.After establishing the biomechanics our temporal bone laboratory, various locations attachments Vibrant transducer were...
To detect prognostic factors for successful sialoendoscopic removal of salivary stones.Retrospective case series.Tertiary referral hospital.Forty-nine consecutive patients who underwent sialoendoscopy sialolithiasis between January 1, 2008, and 2010, at University Hospital Cologne, Germany.Diagnostic interventional using local anesthesia.Stone rate, size, mobility, shape, location, as well clinical follow-up data.Sixty-one percent (39 64) all stones were removed endoscopically. The cutoff...
Objectives/Hypothesis The diagnosis of sialolithiasis is, along with clinical presentation, based on different imaging techniques and more invasive procedures such as sialendoscopy. aim the study was to analyze potential cone beam computed tomography (CBCT) for compare results those sonography Study Design Retrospective analysis. Methods data 43 patients suspected were evaluated retrospectively. All had CBCT sonographic received sialendoscopy confirm possibly treat sialolithiasis. Results...
To investigate the prognostic significance of various factors in hearing preservation after traditional cochlear implantation (CI).Retrospective case review.Academic tertiary referral center.A total 153 implantations with mean patient age at 36 years (from 10 mo to 83 yr) and residual frequencies 250, 500, 1,000 Hz on unaided preoperative pure-tone audiometry were included.CI a conventional full-length electrode.The changes 3 months analyzed regarding patients' demographic factors, shape...
Pleomorphic adenoma (PA) is reported to be the most common benign parotid tumour followed by Warthin (WT), but proportion of these two entities might have changed.Retrospective file analysis.Tertiary referral head and neck centre.Patients who underwent a parotidectomy within period 25 years (1990-2014).Rate occurrence PA WT as well development PA/WT ratio over years.Overall, 1818 patients with (707, 38.9%) (1111, 61.1%) were identified. There was dominance in all years. An increase...
The immune response against cancer is orchestrated by various parameters and site-dependent specificities have been poorly investigated. In our analyses of ten different types, we describe elevated infiltration regulatory T cells as the most common feature, while other lymphocyte subsets also expression immune-regulatory molecules on tumor-infiltrating lymphocytes showed site-specific variation. Multiparametric these data identified similarities renal liver or lung with head neck cancer....