A5102806578- Zebrafish Biomedical Research Applications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Advanced Fluorescence Microscopy Techniques
- Genomics and Chromatin Dynamics
- Developmental Biology and Gene Regulation
- Chromatin Remodeling and Cancer
- Hedgehog Signaling Pathway Studies
- Aquaculture disease management and microbiota
- Vascular Malformations Diagnosis and Treatment
- Intracerebral and Subarachnoid Hemorrhage Research
- Plant Molecular Biology Research
- Angiogenesis and VEGF in Cancer
- Craniofacial Disorders and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Congenital heart defects research
- Cellular Mechanics and Interactions
- Wnt/β-catenin signaling in development and cancer
- Reinforcement Learning in Robotics
- Tracheal and airway disorders
- Fish Ecology and Management Studies
- Cell Adhesion Molecules Research
- Immune Response and Inflammation
- RNA Research and Splicing
- Intracranial Aneurysms: Treatment and Complications
- Polysaccharides and Plant Cell Walls
Baylor College of Medicine
2024-2025
The University of Texas MD Anderson Cancer Center
2016-2023
The University of Texas Health Science Center at Houston
2020
University of Utah
2011-2012
Abstract Epithelial tissues require the removal and replacement of damaged cells to sustain a functional barrier. Dying provide instructive cues that can influence surrounding proliferate, but how these signals are transmitted their healthy neighbors control cellular behaviors during tissue homeostasis remains poorly understood. Here we show dying stem facilitate communication with adjacent by caspase-dependent production Wnt8a-containing apoptotic bodies drive turnover in living epithelia....
Cerebral cavernous malformations (CCMs) are a common type of vascular malformation in the brain that major cause hemorrhagic stroke. This condition has been independently linked to 3 separate genes: Krev1 interaction trapped (KRIT1), 2 (CCM2), and Programmed cell death 10 (PDCD10). Despite commonality disease pathology caused by mutations these genes, we found loss Pdcd10 results significantly different developmental, biological, signaling phenotypes from those seen absence Ccm2 Krit1....
The transcriptional pathways activated downstream of vascular endothelial growth factor (VEGF) signaling during angiogenesis remain incompletely characterized. By assessing the signals responsible for induction Notch ligand delta-like 4 (DLL4) in cells, we find that activation MAPK/ERK pathway mirrors rapid and dynamic DLL4 transcription this is required expression. Furthermore, VEGF/ERK induces phosphorylation ETS ERG, a prerequisite induction. Transcription coincides with ERG-dependent...
ATP-dependent chromatin remodeling protein ATRX is an essential regulator involved in maintenance of DNA structure and state regulation gene expression during development. was originally identified as the monogenic cause X-linked α-thalassemia mental retardation (ATR-X) syndrome. Affected individuals display a variety developmental abnormalities skeletal deformities. Studies from others investigated role development by tissue-specific Atrx knockout. However, impact early has not been...
Epithelia provide a critical protective barrier for our organs and are also the sites where most carcinomas form. Most studies on epithelia use cell culture or organisms high-resolution live imaging is inaccessible without invasive techniques. Here, we introduce developing zebrafish epidermis as an excellent in vivo model system studying living epithelium. We developed tools to fluorescently tag specific epithelial types express genes mosaic fashion using five GAL4 lines identified from...
Severe and often fatal opportunistic fungal infections arise frequently following mucosal damage caused by trauma or cytotoxic chemotherapy. Interaction of pathogens with epithelial cells that comprise mucosae is a key early event associated invasion, and, therefore, enhancing defense mechanisms may mitigate infection. Here, we establish model mold yeast infection mediated inducible cell loss in larval zebrafish. Epithelial extrusion promotes exposure laminin increased attachment, lethality,...
Non-syndromic cleft lip with or without palate (NSCL/P) is a common, severe craniofacial malformation that imposes significant medical, psychosocial and financial burdens. NSCL/P multifactorial disorder genetic environmental factors playing etiologic roles. Currently, only 25% of the variation underlying has been identified by linkage, candidate gene genome-wide association studies. In this study, whole-genome sequencing genotyping followed polygenic risk score (PRS) linkage analyses were...
Here we present the Multisite Assembly of Gateway Induced Clones (MAGIC) system, which harnesses site-specific recombination-based cloning via technology for rapid, modular assembly between 1 and 3 "Entry" vector components, all into a fourth, standard high copy "Destination" plasmid backbone. The MAGIC toolkit spans range in vitro vivo uses, from directing tunable gene expression, to driving simultaneous expression microRNAs fluorescent reporters, enabling recombinase-dependent expression....
Facial development requires a complex and coordinated series of cellular events that, when perturbed, can lead to structural birth defects. A quantitative approach quickly assess morphological changes could address how genetic or environmental inputs differences in facial shape promote malformations. Here, we report on method rapidly analyze craniofacial zebrafish embryos using analytics based coordinate extrapolation system, termed zFACE. Confocal images capture structures morphometric data...
The cellular and molecular cues involved in creating branched tubular networks that transport liquids or gases throughout an organism are not well understood. To identify factors required branching lumen formation of Drosophila tracheal terminal cells, a model for networks, we performed forward genetic-mosaic screen to isolate mutations affecting these processes. From this screen, have identified the first mutation gene Zpr1 (Zinc finger protein 1) by inability Zpr1-mutant cells form...
The zebrafish Danio rerio is a powerful model system to study the genetics of development and disease. However, maintenance husbandry records both time intensive laborious, standardized way manage track large amount unique lines in given laboratory or centralized facility has not been embraced by field. Here, we present FishNET, an intuitive, open-source, relational database for managing data information related maintenance. By creating "virtual facility," FishNET enables users remotely...
Zebrafish are a widely used developmental model because of their transparent embryos and external development. These distinctive characteristics provide valuable insights into embryonic Optical coherence tomography (OCT) offers label-free structural imaging has emerged as preferred tool for imaging. On the other hand, light sheet fluorescence microscopy (LSFM) enables time-lapse molecular multi-hour to multi-day processes due its low phototoxicity photobleaching compared traditional confocal...
Cell elimination by extrusion is important for epithelial homeostasis, but knowing when and where cells will extrude has made in vivo studies difficult. Here, we describe a step-by-step protocol inducing cell from the larval zebrafish epidermis. We detail how to capture dynamics of via time-lapse imaging existing protocols can be implemented analysis shape changes preceding events derivation mechanical measurements associated with these changes. For complete details on use execution this...
The control data provided in Figure 7, E, F, andG, were inadvertently from incorrectly matched samples.
Epithelial tissues sustain barrier function by removing and replacing aberrant or unfit cells. Here, we describe approaches to evaluate epithelial restorative capacity after inducing cell loss in zebrafish larvae. We provide details quantify morphological changes the tail fin epithelium loss, instructions interrogate gene expression proliferation associated with replacement of lost Together, this approach establishes an vivo vertebrate model for rapid assessment molecular pathways...
ABSTRACT Facial development requires a complex and coordinated series of cellular events, that when perturbed, can lead to structural birth defects. A standardized quantitative approach quickly assess morphological changes could address how genetic or environmental inputs differences in facial development. Here we report on method rapidly analyze craniofacial zebrafish embryos combines simple staining mounting paradigm with F acial nalytics based C oordinate E xtrapolation system, termed...
Epithelia provide the first line of defense against foreign pathogens, and disruption tissue homeostasis frequently allows for opportunistic infections. Here we a protocol induction epithelial cell loss in zebrafish larvae, followed by infection with fungal pathogens. Details are provided monitoring larval survival after infection, assessment burden, prophylactic treatment antifungal compounds. Limitations include potential toxicity high inoculums to induce lethal some pathogenic species....
ABSTRACT The zebrafish Danio rerio is a powerful model system to study the genetics of development and disease. However, maintenance husbandry records both time intensive laborious, standardized way manage track large amount unique lines in given laboratory or centralized facility has not been embraced by field. Here we present FishNet, an intuitive, open source, relational database for managing data information related maintenance. By creating “virtual facility”, FishNET enables users...
Abstract Background Efficient animal models are needed in order to investigate fungal pathogenicity and antifungal therapy the context of epithelial injury, e.g., due anticancer chemotherapy. Using a Gal4 enhancer trap (GET) zebrafish line facilitating metronidazole (MTZ)-inducible ablation (periderm) cells, we aimed establish mucositis model predisposing larvae for invasion. Methods 4 days post-fertilization Et(Gal4-VP16)zc1044A;Tg(UAS-1b:nfsB-mCherry)c264 were exposed 10 mM MTZ E3 medium 5...
Severe, often fatal, opportunistic fungal infections frequently arise following mucosal damage caused by trauma or cytotoxic chemotherapy. Interaction of pathogens with epithelial cells that comprise mucosa is a key early event associated invasion, and therefore, enhancing defense mechanisms may mitigate infection. Here we establish model mold yeast infection mediated inducible cell loss in larval zebrafish. Epithelial extrusion promoted exposure laminin was increased attachment, invasion...