A5063840639- Pluripotent Stem Cells Research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Acute Myeloid Leukemia Research
- Air Quality and Health Impacts
- Cancer-related molecular mechanisms research
- Atmospheric Ozone and Climate
- Platelet Disorders and Treatments
- Immune cells in cancer
- 3D Printing in Biomedical Research
- Angiogenesis and VEGF in Cancer
- Renal and related cancers
- Atmospheric chemistry and aerosols
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Erythrocyte Function and Pathophysiology
- Endoplasmic Reticulum Stress and Disease
- Tissue Engineering and Regenerative Medicine
- Atmospheric and Environmental Gas Dynamics
- Electron Spin Resonance Studies
- Neurogenesis and neuroplasticity mechanisms
- Extracellular vesicles in disease
- Apelin-related biomedical research
- Genetics, Aging, and Longevity in Model Organisms
- Parkinson's Disease Mechanisms and Treatments
Baylor College of Medicine
2021-2023
Quantitative BioSciences
2022-2023
Yantai Institute of Coastal Zone Research
2022
Chinese Academy of Sciences
2022
Alnylam Pharmaceuticals (United States)
2022
Biogen (United States)
2016-2020
University of Toronto
2006-2017
Heart and Stroke Foundation
2013-2017
University Health Network
2013-2017
Toronto General Hospital
2017
The transcriptional pathways activated downstream of vascular endothelial growth factor (VEGF) signaling during angiogenesis remain incompletely characterized. By assessing the signals responsible for induction Notch ligand delta-like 4 (DLL4) in cells, we find that activation MAPK/ERK pathway mirrors rapid and dynamic DLL4 transcription this is required expression. Furthermore, VEGF/ERK induces phosphorylation ETS ERG, a prerequisite induction. Transcription coincides with ERG-dependent...
Significance Parkinson’s disease (PD) is the second most prevalent neurodegenerative of aging, affecting approximately 10 million patients worldwide with no approved therapies to modify progression disease. Further understanding cellular mechanisms contributing development PD necessary discover therapies. Here, we characterize role a recently identified GWAS hit for sporadic PD, ITPKB, in aggregation α-synuclein, primary pathological feature These results identify inhibition...
Uromodulin-associated kidney disease (UAKD) is caused by mutations in the uromodulin (UMOD) gene that result a misfolded form of UMOD protein, which normally secreted nephrons. In UAKD patients, mutant poorly and accumulates ER distal epithelium, but its role progression largely unknown. Here, we modeled accumulation mice expressing murine equivalent human p.Cys148Trp point mutation (UmodC147W/+ mice). Like affected humans, these UmodC147W/+ developed spontaneous progressive with organ...
Maintenance of vascular integrity is required for embryogenesis and organ homeostasis. However, the gene expression programs that stabilize blood vessels are poorly understood. Here, we show histone methyltransferase Ezh2 maintains developing vasculature by repressing a transcriptional program activates Mmp9. Inactivation in mouse endothelium caused embryonic lethality with compromised increased extracellular matrix degradation. Genome-wide approaches showed targets Mmp9 its activators Fosl1...
Pericytes are tissue-resident mesenchymal progenitor cells anatomically associated with the vasculature that have been shown to participate in tissue regeneration. Here, we tested hypothesis kidney pericytes, derived from FoxD1+ mesodermal progenitors during embryogenesis, necessary for postnatal homeostasis. Diphtheria toxin delivery FoxD1Cre::RsDTR transgenic mice resulted selective ablation of >90% pericytes but not other cell lineages. Abrupt increases plasma creatinine, blood urea...
Loss of the microvascular (MV) network results in tissue ischemia, loss function, and is a hallmark chronic diseases. The incorporation functional vascular with that host remains challenge to utilizing engineered tissues clinically relevant therapies. We showed vascular-bed-specific endothelial cells (ECs) exhibit differing angiogenic capacities, kidney (MVECs) being most deficient, sought explore underlying mechanism. Constitutive activation phosphatase PTEN MVECs resulted impaired PI3K/AKT...
We report our findings on the assembly of HIV-1 protein Vpu into soluble oligomers. is a key protein. It has been considered exclusively single-pass membrane Previous observations show that this forms stable oligomers in aqueous solution, but details about these still remain obscure. This an interesting and rather unique observation, as number proteins transitioning between embedded states limited. In study we made use engineering, size exclusion chromatography, cryoEM electron paramagnetic...
Abstract. The Orbiting Carbon Observatory-2 (OCO-2) is the first National Aeronautics and Space Administration (NASA) satellite designed to measure atmospheric carbon dioxide (CO2) with accuracy, resolution, coverage needed quantify CO2 fluxes (sources sinks) on regional scales. OCO-2 was successfully launched 2 July 2014, joined 705 km Afternoon Constellation 3 August 2014. On monthly time scales, 7 12 % of these measurements are sufficiently cloud aerosol free yield estimates...
The kinetics of the reactions hydroxyl radical with n-octane (k1), n-nonane (k2), and n-decane (k3) at 240-340 K a total pressure approximately 1 Torr has been studied using relative rate combined discharge flow mass spectrometer (RR/DF/MS) technique. constant for these was found to be positively dependent on temperature, an Arrhenius expression k1 = (2.27 +/- 0.21) x 10(-11)exp[(-296 27)/T], k2 (4.35 0.49) 10(-11)exp[(-411 32)/T], k3 (2.26 0.28) 10(-11)exp[(-160 36)/T] cm3 molecule(-1)...
Inducing a stable and predictable program of neural cell fate in pluripotent cells vitro is an important goal for utilizing these modeling human disease mechanisms. However, the extent to which specification recapitulates vivo remains be fully established. We previously demonstrated that mouse embryo, activation fibroblast growth factor (FGF) signalling promotes definitive stem (NSC) development through upregulation transcription Zfhx1b. Here, we asked whether Zfhx1b similarly required...
Mouse definitive neural stem cells (NSCs) are derived from a population of LIF-responsive primitive (pNSCs) within the neurectoderm, yet details on early signaling and transcriptional mechanisms that control this lineage transition lacking. Here we tested whether FGF Wnt pathways can regulate Zfhx1b expression to cell development. By microinjecting FGF8b into pro-amniotic cavity ex vivo at 7.0 days post-coitum (dpc) culturing whole embryos, demonstrate neurectoderm-specific gene (for...
NASA’s Soil Moisture Active Passive (SMAP) mission has been validating its soil moisture (SM) products since the start of data production on March 31, 2015. Prior to launch, defined a set criteria for core validation sites (CVS) that enable testing key SM accuracy requirement (unbiased root-mean-square error <0.04 m<sup>3</sup>/m<sup>3</sup>). The approach also includes other (“sparse network”) in situ measurements, satellite products, model-based and field...
Abstract Early diagnosis of acute myeloid leukemia (AML) in the pre-leukemic stage remains a clinical challenge, as patients show no symptoms, lacking any known morphological or numerical abnormalities blood cells. Here, we demonstrate that platelets with structurally abnormal mitochondria emerge at phase AML, preceding detectable changes cell counts detection leukemic blasts blood. We visualized frozen-hydrated from mice different time points during AML development situ using electron...
A significant challenge in the electroencephalogram EEG lies fact that current data representations involve multiple electrode signals, resulting redundancy and dominant lead information. However extensive research conducted on classification focuses designing model architectures without tackling underlying issues. Otherwise, there has been a notable gap addressing preprocessing for EEG, leading to considerable computational overhead Deep Learning (DL) processes. In light of these issues, we...