A5102706101- Craniofacial Disorders and Treatments
- Bone health and treatments
- Cleft Lip and Palate Research
- Bone and Joint Diseases
- Cancer Diagnosis and Treatment
- dental development and anomalies
- Neurological Disease Mechanisms and Treatments
- Prenatal Substance Exposure Effects
- Pregnancy and preeclampsia studies
- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Bone Metabolism and Diseases
- Bone fractures and treatments
- Vitamin D Research Studies
- Birth, Development, and Health
- IL-33, ST2, and ILC Pathways
- Anesthesia and Neurotoxicity Research
- Extracellular vesicles in disease
- Osteoarthritis Treatment and Mechanisms
- Bone Tumor Diagnosis and Treatments
- MicroRNA in disease regulation
- Bone health and osteoporosis research
- Bone and Dental Protein Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Bone Tissue Engineering Materials
Western University of Health Sciences
2020-2021
Augusta University
2011-2020
Georgia Regents Medical Center
2013-2015
Augusta University Health
2012-2013
Chronic bone degenerative diseases represent a major threat to the health and well-being of population, particularly those with advanced age. This study isolated exosomes (EXO), natural nano-particles, from dendritic cells, "directors" immune response, examine immunobiology DC EXO in mice, their ability reprogram cells responsible for experimental alveolar loss vivo. Distinct subtypes including immune-regulatory (regDC EXO), loaded TGFB1 IL10 after purification, along stimulatory (stimDC...
Vascular contributions to cognitive impairment and dementia (VCID) make up 50% of the cases dementia. The purpose this study was determine effect chronic remote ischemic conditioning (C-RIC) on improving long-term (6 months) outcomes cerebral blood flow (CBF) collateral formation in a mouse model VCID. Adult C57BL/6J male mice (10 weeks) were randomly assigned four different groups: (1) sham-bilateral carotid artery stenosis (BCAS), (2) BCAS + sham RIC, (3) BCAS+C-RIC for 1 month (1MO), (4)...
The incidence of craniosynostosis is one in every 1,800-2500 births. gene-environment model proposes that if a genetic predisposition coupled with environmental exposures, the effects can be multiplicative resulting severely abnormal phenotypes. At present, very little known about role interactions modulating phenotypes, but prior evidence suggests for endocrine factors. Here we provide report thyroid hormone exposure on murine calvaria cells. Murine derived cells were exposed to critical...
The purpose of this study was to develop a rat model predictive bisphosphonate-related osteonecrosis the jaw (BRONJ) after exodontias. Thirty female rats were randomized into 2 groups, control and experimental. experimental group received intravenous injections zoledronate (20 μg/kg). mesial root right mandibular first molar extracted. Rats euthanized at 0, 4, 8 weeks. Bone mineral density (BMD), collagen breakdown (pyridinium [PYD]), vascular regeneration (VEGF), histology examined. A trend...
Background Selective serotonin reuptake inhibitor (SSRI) use in pregnancy has been linked to craniofacial birth defects. Little is known about the effects of or SSRIs on development. Here, we provide evidence that citalopram alters osteogenic profile murine calvarial cells and leads dysmorphology. Methods We used mouse pre‐osteoblast (MC3T3‐E1) study biochemical (microarray quantitative reverse transcription polymerase chain reactions) after treatment with a titrated dose citalopram. C57BL‐6...
Innate lymphoid cells (ILCs) are master regulators of immune and inflammatory responses but their own regulatory mechanisms functional roles subtypes (i.e., ILC1s-ILC3s) remain largely unresolved. Interestingly, AMP-Activated Protein Kinase (AMPK), influences its role in modulation ILCs is not known. Periodontitis a prevalent disorder with impairment contributing importantly to pathogenesis; however, neither the nor AMPK has been explored this condition. We tested hypotheses that a)...
Large scale surveillance studies, case as well cohort studies have identified the influence of thyroid hormones on calvarial growth and development. Surveillance data suggests maternal disorders (hyperthyroidism, hypothyroidism with pharmacological replacement, Maternal Graves Disease) are linked to much a 2.5 fold increased risk for craniosynostosis. Craniosynostosis is premature fusion one or more sites (sutures) prior completion brain expansion. Thyroid maintain proper bone mineral...
Mechanisms leading to age-related reductions in bone formation and subsequent osteoporosis are still incompletely understood. We recently demonstrated that kynurenine (KYN), a tryptophan metabolite, accumulates serum of aged mice induces loss. Here, we report on novel mechanisms underlying KYN's detrimental effect aging. show KYN is increased with aging murine marrow mesenchymal stem cells (BMSCs). reduces via modulating levels CXCL12 its receptors as well histone deacetylase 3 (Hdac3)....
Bone has the potential for spontaneous healing. This process, however, often fails in patients with comorbidities. Tissue engineering combining functional cells, biomaterials and osteoinductive cues may provide alternative treatment strategies. We have recently demonstrated that stromal cell-derived factor-1β (SDF-1β) works concert bone morphogenetic protein-2 (BMP-2) to potentiate osteogenic differentiation of marrow-derived mesenchymal stem/stromal cells (BMSCs). Here, we test hypothesis...
Long-term use of intravenous bisphosphonates, such as zoledronic acid (zoledronate), has been linked to bisphosphonate-related osteonecrosis the jaw (BRONJ). Invasive dental surgery seems trigger bone necrosis in most cases. To determine effects on vascular structure rat mandible. Extracted mandibular first molar rats that received 2 IV injections zoledronate (20 μg/kg), 4 weeks apart. Zoledronate-treated (n = 18) were then compared a control group untreated 18). At fourth, eighth, and 12th...
Abstract Bone has the potential for spontaneous healing. However, this process often fails in patients with co‐morbidities requiring clinical intervention. Numerous studies have revealed that bone marrow‐derived mesenchymal stem/stromal cells (BMSCs) hold great regenerative therapies. Common problems include poor cell engraftment, which can be addressed by irradiation prior to transplantation. Increasing evidence suggests stromal cell‐derived factor‐1 (SDF‐1) is involved formation....
The use of selective serotonin reuptake inhibitors (SSRIs) for the treatment depression during pregnancy is suggested to increase incidence craniofacial abnormalities including craniosynostosis. Little known about this mechanism, however based on previous data we propose a mechanism that affects cell cycle. Excessive proliferation, and reduction in apoptosis may lead hyperplasia within suture allow differentiation, bony infiltration, fusion. Here utilized vivo vitro analysis investigate...
An estimated 3% of US pregnancies are affected by maternal thyroid dysfunction, with between one and three every 1000 being complicated overactive levels. Excess hormones linked to neurological impairment excessive craniofacial variation, affecting both endochondral intramembranous bone. Using a geometric morphometric approach, this study evaluates the role in utero thyroxine overexposure on growth offspring mandibles sample 241 mice. Canonical variate analysis utilized 16 unilateral...
Craniosynostosis, the premature fusion of one or more cranial sutures, is estimated to occur in 1:1800 2500 births. Genetic murine models craniosynostosis exist, but often imperfectly model human patients. Case, cohort, and surveillance studies have identified excess thyroid hormone as an agent that can either cause exacerbate cases craniosynostosis.Here we investigate influence utero vitro exogenous exposure on a craniosynostosis, Twist 1 +/-.By 15 days post-natal, there was evidence...
OPEN ACCESSMay 2, 2012Early Tooth Development Mohammed Elsalanty, MD, PhD Medical College of Georgia at Regents University Google Scholar More articles by this author https://doi.org/10.15766/mep_2374-8265.9169 SectionsAbout ToolsDownload Citations ShareFacebookTwitterEmail Abstract Introduction: The challenge teaching the subject tooth development lies in explanation a complicated three-dimensional process occurring within small, confined space over period time. Further, there are few...
Craniosynostosis (CS) is the premature fusion of one or more cranial sutures; it can both hinder growth brain and result in severe craniofacial dysmorphology. Although several genes have been implicated with CS, unclear how these genotypes lead to widely variable phenotypes. One possibility gene‐environment interaction. A CDC study found environmental risk factors associated including maternal hyperthyroidism. We hypothesize that predisposed will extreme CS phenotypes when exposed thyroid...
Craniosynostosis (CS) is the premature fusion of one or more fibrous joints skull, which normally allow for growth neurocranium. The incidence CS in every 2000–3000 births. involves overgrowth bone at osteogenic fronts developing cranial bones. etiology CS, whether genetic environmental, unknown majority cases. Little known about role environmental agents gene‐environment interactions modulating phenotypes. clinical and epidemiological literature suggests that maternal hyperthyroidism a risk...
Angiotensin signaling is known to be sexually dimorphic and although a well‐studied target for intervention in stroke cognitive impairment males, studies females are rare. With suffering disproportionately greater negative impact of dementia vs. effective interventions utmost urgency. The aim the current study was determine stimulation angiotensin receptor subtype 2 (AT2R) with compound 21 (C21) on development post‐stroke after experimental ischemic stroke. Ovariectomized (OVX),...