A5004084013- Heat shock proteins research
- Tryptophan and brain disorders
- Vitamin D Research Studies
- Peptidase Inhibition and Analysis
- Genomics, phytochemicals, and oxidative stress
- Stress Responses and Cortisol
- Chemotherapy-induced organ toxicity mitigation
- Advanced Glycation End Products research
- MicroRNA in disease regulation
- Natural product bioactivities and synthesis
- Heme Oxygenase-1 and Carbon Monoxide
- Cancer-related molecular mechanisms research
- Crystallization and Solubility Studies
- Synthesis and biological activity
- Drug Transport and Resistance Mechanisms
- Bioactive Compounds and Antitumor Agents
- Ubiquitin and proteasome pathways
- Acute Kidney Injury Research
- X-ray Diffraction in Crystallography
- Hepatitis B Virus Studies
- Inflammatory Bowel Disease
- Neuropeptides and Animal Physiology
- Paraoxonase enzyme and polymorphisms
- Diabetes Treatment and Management
- Medicinal Plants and Bioactive Compounds
Mansoura University
2014-2025
National Cancer Center of Georgia
2019-2025
Augusta University Health
2017-2023
Augusta University
2019-2023
Ralph H. Johnson VA Medical Center
2019-2022
Medical University of South Carolina
2019-2022
Molecular Oncology (United States)
2018
There is increasing evidence of the involvement tryptophan metabolite kynurenine (KYN) in disrupting osteogenesis and contributing to aging-related bone loss. Here, we show that KYN has an effect on resorption by osteoclastogenesis. We have previously reported vivo treatment with significantly increased osteoclast number lining surfaces. report direct receptor activator nuclear factor kappa-B ligand (RANKL)-induced osteoclastogenesis Raw 264.7 macrophage cells, propose a potential mechanism...
Diabetic nephropathy (DN) is a diabetic complication characterized by disruption of renal microvasculature, reactive oxygen species accumulation and increased inflammation, all which contribute to injury. Phenethyl isothiocyanate (PEITC) naturally occurring well known for its antioxidant anti-inflammatory effects, yet reno-preventive effects against DN has not been investigated. The current study looked into the in vivo reno-protective PEITC STZ-induced rats. (3, 10 30 mg/kg) was...
Abstract UNC45A is a key co-chaperone supporting cancer cell proliferation and tumorigenesis. Interestingly, essential for cells but not normal proliferation. However, the mechanism underlying regulatory function well studied. We have previously shown that functions as glucocorticoid receptor (GR) co-activator to drive mitotic kinase NEK7 gene transcription. centrosome separation, spindle formation, cytokinesis. GR has been implicated in promoting aggressive prostate tumor growth resistance...
Abstract Heat shock protein 90 (Hsp90) is a molecular chaperone that plays key role in protecting the proteome. In cancer cells, this Hsp90 function hijacked to promote cell proliferation. Except for Pimitespib (TAS-116), approved clinical use Japan 2022, no inhibitors have yet been by FDA. One of main side effects current tested clinic their induction heat response (HSR). Therefore, identifying new destabilize oncoproteins clients without inducing HSR an attractive strategy harness...
Cumulative evidence suggests that the heat shock protein 90 (Hsp90) co-chaperone UNC-45 myosin chaperone A (UNC45A) contributes to tumorigenesis and its expression in cancer cells correlates with proliferation metastasis of solid tumors. However, molecular mechanism by which UNC45A regulates cell remains largely unknown. Here, using siRNA-mediated gene silencing various human cells, we report is essential for breast growth, but dispensable normal proliferation. Immunofluorescence microscopy,...
Low response rates and immune-related adverse events limit the remarkable impact of cancer immunotherapy. To improve clinical outcomes, preclinical studies have shown that combining immunotherapies with N-terminal Hsp90 inhibitors resulted in improved efficacy, even though induction an extensive heat shock (HSR) less than optimal dosing these limited their efficacy as monotherapies. We discovered natural product Enniatin A (EnnA) targets destabilizes its client oncoproteins without inducing...
Mechanisms leading to age-related reductions in bone formation and subsequent osteoporosis are still incompletely understood. We recently demonstrated that kynurenine (KYN), a tryptophan metabolite, accumulates serum of aged mice induces loss. Here, we report on novel mechanisms underlying KYN's detrimental effect aging. show KYN is increased with aging murine marrow mesenchymal stem cells (BMSCs). reduces via modulating levels CXCL12 its receptors as well histone deacetylase 3 (Hdac3)....
Aim Hepatocellular carcinoma (HCC) is the fifth most common cancer in men and seventh women. HCC varies widely incidence through world, with rising Egypt. This study aimed to estimate serum levels of matrix metalloproteinase-9 (MMP-9) its substrate galectin-3 order evaluate their diagnostic accuracy relation HCC-related clinical features. Methods For this purpose, these biochemical markers were assessed 50 patients, 30 cirrhotic patients addition 10 healthy subjects as a control group using...
The present study aims to investigate the in vivo and vitro anti-tumour properties of phenethyl isothiocyanate (PEITC) alone combination with doxorubicin (Dox). activity was evaluated by MTT assay using cultured human breast cancer cell line (MCF-7) hepatoma (HepG-2) lines. In vivo, Ehrlich solid tumour model used. Tumour volume, weight antioxidant parameters were determined. Immunohistochemistry analysis for active (cleaved) caspase-3 also performed. We tested effect PEITC treatment on...
Renal toxicity is a serious side effect that hinders the use of cisplatin, commonly used and effective chemotherapeutic agent. Meanwhile, quinacrine an FDA approved drug has been stated for its anti-inflammatory effect. Thus, we investigated ameliorative against cisplatin-induced renal toxicity. Single intraperitoneal (i.p.) 10 mg/kg cisplatin administration induced injury in rats. Our results showed mg/kg/day decreased mortality rate rats from 46.15% (cisplatin group) to 12.5%,...
The development of the field nanotechnology has revolutionized various aspects in fields modern sciences. Nano-medicine is one primary for application techniques. current study sheds light on reno-protective impacts gold nano-particles; nanogold (AuNPs) against 5-flurouracil (5-FU)-induced renal toxicity. Indeed, use 5-FU been associated with kidney injury which greatly curbs its therapeutic application. In study, injection was a significant escalation indices injury, i.e., creatinine and...
Abstract D‐Ring‐ seco ‐limonoids (tetranortriterpenoids), such as gedunin and xylogranin B display anti‐cancer activity, acting via inhibition of Hsp90 and/or associated chaperon machinery (e.g., p23). Despite this, these natural products have received relatively little attention, both in terms an enabling synthetic approach (which would allow access to derivatives), a consequence their structure–activity relationship (SAR). Disclosed herein is generally applicable route the BCD ring system...
Bone marrow skeletal stem cells (SSCs) secrete many cytokines including stromal derived factor-1 or CXCL12, which influences cell proliferation, migration, and differentiation. All CXCL12 splice variants are rapidly truncated on their N-terminus by dipeptidyl peptidase 4 (DPP4). This includes the common variant alpha (1–68) releasing a much less studied metabolite CXCL12(3–68). Here, we found that CXCL12(3–68) significantly inhibited SSC osteogenic differentiation RAW-264.7 osteoclastogenic...