A5090938895- Vector-borne infectious diseases
- Microbial infections and disease research
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Insect and Pesticide Research
- Insect symbiosis and bacterial influences
- Herpesvirus Infections and Treatments
- Aquaculture disease management and microbiota
- Mycobacterium research and diagnosis
- Cancer-related molecular mechanisms research
- Insect Resistance and Genetics
- Animal Disease Management and Epidemiology
- Insect Pest Control Strategies
- Malaria Research and Control
- Bacteriophages and microbial interactions
- Pneumonia and Respiratory Infections
- Respiratory viral infections research
- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- Escherichia coli research studies
- Genomic variations and chromosomal abnormalities
- Prion Diseases and Protein Misfolding
- Toxoplasma gondii Research Studies
- Salmonella and Campylobacter epidemiology
- Trypanosoma species research and implications
United States Department of Agriculture
2014-2024
Agricultural Research Service
2015-2024
Barron Associates (United States)
2018
Alaska Department of Environmental Conservation
2018
Navajo Nation Division of Health
2018
Navajo Technical University
2018
University of Alaska Fairbanks
2018
Washington State University
2006-2014
Pacific Northwest National Laboratory
2010
University of Washington
2010
Babesia bovis is an apicomplexan tick-transmitted pathogen of cattle imposing a global risk and severe constraints to livestock health economic development. The complete genome sequence was undertaken facilitate vaccine antigen discovery, allow for comparative analysis with the related hemoprotozoa Theileria parva Plasmodium falciparum. At 8.2 Mbp, B. similar in size that spp. Structural features T. genomes are remarkably similar, extensive synteny present despite several chromosomal...
The rickettsia Anaplasma marginale is the most prevalent tick-borne livestock pathogen worldwide and a severe constraint to animal health. A. establishes lifelong persistence in infected ruminants these animals serve as reservoir for ticks acquire transmit pathogen. Within mammalian host, generates antigenic variants by changing surface coat composed of numerous proteins. By sequencing annotating complete 1,197,687-bp genome St. Maries strain , we show that this dominated two families...
Large multidrug resistance plasmids of the A/C incompatibility complex (IncA/C) have been found in a diverse group Gram-negative commensal and pathogenic bacteria. We present three completed sequences from IncA/C that originated Escherichia coli (cattle) Salmonella enterica serovar Newport (human) carry cephamycinase gene blaCMY-2. These large (148 to 166 kbp) share extensive sequence identity synteny. The most divergent plasmid, peH4H, has lost several conjugation-related genes gained...
Abstract Background Transmission of arthropod-borne apicomplexan parasites that cause disease and result in death or persistent infection represents a major challenge to global human animal health. First described 1901 as Piroplasma equi , this re-emergent parasite was renamed Babesia subsequently Theileria reflecting an uncertain taxonomy. Understanding mechanisms by which evade immune chemotherapeutic elimination is required for development effective vaccines chemotherapeutics. The...
A novel apicomplexan parasite was serendipitously discovered in horses at the United States – Mexico border. Phylogenetic analysis based on 18S rDNA showed erythrocyte-infective to be related to, but distinct from, Theileria spp. Africa, most similar taxa being from waterbuck and mountain zebra. The degree of sequence variability observed locus also suggests likely existence additional cryptic species. Among described species, genome this equid is that equi, a pathogen horses. estimated...
Theileria orientalis is a tick-borne hemoparasite that causes anemia, ill thrift, and death in cattle globally. The Ikeda strain of T. more virulent than other strains, leading to severe clinical signs up 5% affected animals. Within the Asia-Pacific region, where it affects 25% Australian cattle, has significant economic impact on industry. In 2017, was detected herd Albermarle County, Virginia, United States. Months earlier, U.S. alerted invasion Asian longhorned tick, Haemaphysalis...
Bronchopneumonia is a population-limiting disease in bighorn sheep much of western North America. Previous investigators have isolated diverse bacteria from the lungs affected sheep, but no single bacterial species consistently present, even within epizootics. We obtained high-quality diagnostic specimens nine pneumonic three populations and analyzed present bronchoalveolar lavage seven by using culture-independent method (16S rRNA gene amplification clone library analyses). Mycoplasma...
Genome rearrangements are important in evolution, cancer, and other diseases. Precise mapping of the is essential for identification involved genes, many techniques have been developed this purpose. We show here that end-sequence profiling (ESP) particularly well suited to ESP accomplished by constructing a bacterial artificial chromosome (BAC) library from test genome, measuring BAC end sequences, pairs onto normal genome sequence. Plots end-sequences density identify copy number...
Babesia bovis, is a tick borne apicomplexan parasite responsible for important cattle losses globally. parasites have complex life cycle including asexual replication in the mammalian host and sexual reproduction vector. Novel control strategies aimed at limiting transmission of are needed, but blocking vaccine candidates remain undefined. Expression HAP2 has been recognized as critical fertilization Babesia-related Plasmodium, leading candidate against malaria. Hereby we identified B. bovis...
Abstract Background With the recent completion of numerous sequenced bacterial genomes, notable advances have been made in understanding level conservation between various species. However, relatively little is known about genomic diversity among strains. We determined complete genome sequence Florida strain Anaplasma marginale , and near (>96%) sequences for an additional three strains, comparative analysis with previously fully St. Maries genome. Results These comparisons revealed that...
ABSTRACT Anaplasma marginale subsp. centrale is a naturally attenuated subtype that has been used as vaccine for century. We sequenced the genome of this organism and compared it to those virulent senso stricto A. strains. The comparison markedly narrows number outer membrane protein candidates development safer inactivated provides insight into diversity among strains lato .
Babesia bovis, an intra-erythrocytic tick-borne apicomplexan protozoan, is one of the causative agents bovine babesiosis. Its life cycle includes sexual reproduction within cattle fever ticks, Rhipicephalus spp. Six B. bovis 6-Cys gene superfamily members were previously identified (A, B, C, D, E, F) where their orthologues in Plasmodium parasite have been shown to encode for proteins required development stages. The current study four additional genes (G, H, I, J) genome. These are...
Throughout their life cycle, Babesia parasites alternate between a mammalian host, where they cause babesiosis, and the tick vector. Transition hosts results in distinct environmental signals that influence patterns of gene expression, consistent with morphological functional changes operating during stages. In addition, comparing differential expression among parasite stages can provide clues for developing improved methods control. Hereby, we upgraded genome assembly bovis, bovine...
Theileria orientalis Ikeda has caused an epidemic of bovine anemia and abortion across several U.S. states. This apicomplexan hemoparasite is transmitted by Haemaphysalis longicornis ticks; however, it unknown if other North American ticks are competent vectors. Since the disease movement largely determined host tick range(s), prediction T. spread among cattle populations requires determination additional Although Rhipicephalus microplus mostly been eradicated from U.S., outbreaks in occur...
Introduction Theileria orientalis , an economically significant tick-borne hemoparasite, infects cattle globally. The T. Ikeda genotype, transmitted by Haemaphysalis longicornis ticks, is associated with clinical manifestations characterized anemia, abortions, and mortality, although subclinical infections prevail. Despite the common occurrence of infections, therapeutic interventions targeting in such cases are currently lacking, impeding effective parasite control measures. To address this...
The rhadinovirus Ovine herpesvirus 2 (OvHV-2) is the causative agent of sheep-associated malignant catarrhal fever. OvHV-2 primarily affects ruminants and has a worldwide distribution. In this study, composite sequence genomic DNA isolated from nasal secretions sheep experiencing virus-shedding episodes was determined compared with lymphoblastoid cell line derived clinically affected cow. study confirmed information for line-isolated showed no apparently significant changes in genome during...
This report describes the genome sequences of Mannheimia haemolytica serotype A2 isolated from pneumonic lungs two different ruminant species, one Ovis aries, designated ovine (O), and other Bos taurus, bovine (B).
Mycoplasma ovipneumoniae contributes to polymicrobial pneumonia in domestic sheep. Elucidation of host genetic influences M . nasal detection has the potential reduce incidence sheep through implementation selective breeding strategies. Nasal mucosal secretions were collected from 647 a large US flock. Ewes three breeds (Polypay n = 222, Rambouillet 321, and Suffolk 104) ranging age one seven years, sampled at different times production cycle (February, April, September/October) over four...
A genome-wide association study (GWAS) was performed to investigate seven red blood cell (RBC) phenotypes in over 500 domestic sheep (Ovis aries) from three breeds (Columbia, Polypay, and Rambouillet). single nucleotide polymorphism (SNP) showed significant with increased mean corpuscular hemoglobin concentration (MCHC, P = 6.2×10(-14)) suggestive decreased volume (MCV, 2.5×10(-6)). The ovine HapMap project found the same genomic region peak SNP has been under extreme historical selective...
Strain superinfection occurs when a second pathogen strain infects host already infected with primary strain. The selective pressures that drive divergence, which underlies superinfection, and allow penetration of new into population are critical knowledge gaps relevant to shifts in infectious disease epidemiology. In regions endemicity high prevalence infection, broad immunity develops against Anaplasma marginale, highly antigenically variant rickettsial pathogen, creates strong pressure...
This study examines the patterns of gene integration gfp-bsd upon stable transfection into T3Bo strain Babesia bovis using a plasmid designed to integrate homologous sequences parasite's two identical ef-1α A and B genes. While transfected BboTf-149-6 cell line displayed distinct integration, clonal lines derived from this by sorting contained only single insertions. Whole genome sequencing selected lines, E9 C6, indicated insertion occurring legitimate recombination mechanisms: one expected...
Mycoplasma ovis is a hemotropic bacterium reported to infect sheep, goats, and deer species. Infection in these species can result anemia, jaundice, ill-thrift. Although of worldwide distribution, only rare reports this the United States exist. The objectives retrospective study were identify prevalence distribution M. ovis, associated demographic management risk factors, reproductive production outcomes with infection on domestic sheep (Ovis aries) operations States. As part Department...
Babesia bovis is a hemoprotozoan parasite of cattle that has complex life cycle within vertebrate and invertebrate hosts. In the mammalian host, B. undergoes asexual reproduction while in tick midgut, gametes are induced, fuse, form zygotes. The zygote infects gut epithelial cells transform into kinetes released hemolymph invade other tissues such as ovaries, resulting transovarial transmission to offspring. To compare gene regulation between different stages, we collected parasites...