A5053487335- Ubiquitin and proteasome pathways
- Blood Coagulation and Thrombosis Mechanisms
- Bladder and Urothelial Cancer Treatments
- Lung Cancer Treatments and Mutations
- Venous Thromboembolism Diagnosis and Management
- Cancer-related Molecular Pathways
- Hormonal and reproductive studies
- Colorectal Cancer Treatments and Studies
- Sexual function and dysfunction studies
- Respiratory Support and Mechanisms
- Neonatal Respiratory Health Research
- Histone Deacetylase Inhibitors Research
- Protease and Inhibitor Mechanisms
- Platelet Disorders and Treatments
- Endoplasmic Reticulum Stress and Disease
- Sepsis Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Urinary Bladder and Prostate Research
- Spine and Intervertebral Disc Pathology
- Cancer, Lipids, and Metabolism
- Lung Cancer Diagnosis and Treatment
- Radiomics and Machine Learning in Medical Imaging
- Cell death mechanisms and regulation
- Cell Adhesion Molecules Research
- Bone Metabolism and Diseases
Chang Gung University
2015-2024
Chiayi Chang Gung Memorial Hospital
2013-2024
Memorial Hospital of South Bend
2022-2023
Kaohsiung Chang Gung Memorial Hospital
2022
Tokai University Hospital
2022
Chang Gung Memorial Hospital
2020-2022
National Chiayi University
2019
Peking University First Hospital
2019
Peking University
2019
Institute of Physiology and Basic Medicine
2013
Thrombomodulin (TM) is an integral membrane glycoprotein that a potent anticoagulant factor. TM may also possess functions distinct from its activity. Here the influence of on cell adhesion was studied in TM-negative melanoma A2058 cells transfected with green fluorescent protein-tagged (TMG) or lectin domain-deleted (TMG(ΔL)). Confocal microscopy demonstrated both TMG and TMG(ΔL) were distributed plasma membrane. TMG-expressed grew as closely clustered colonies, localized prominently...
Evaluation of the protective effects curcuminoid (curcumin and bisdemethoxycurcumin)-loaded liposomes against bone turnover in a cell-based model osteoarthritis Chih-Chang Yeh,1,2 Yu-Han Su,3 Yu-Jhe Lin,3 Pin-Jyun Chen,3 Chung-Sheng Shi,1 Cheng-Nan Chen,3,* Hsin-I Chang3,* 1Graduate Institute Clinical Medical Sciences, College Medicine, Chang Gung University, Taoyuan 333, Taiwan, Republic China; 2Orthopaedic Department, Chiayi Branch, Taichung Veterans General Hospital, Chiayi, 3Department...
The stress-upregulated catecholamines-activated β1- and β2-adrenergic receptors (β1/2-ARs) have been shown to accelerate the progression of cancers such as colorectal cancer (CRC). We investigated underlying mechanism inhibition β1/2-ARs signaling for treatment CRC elucidated significance β2-AR expression in vitro clinical samples. impacts β1/2-AR antagonists CRC-xenograft vivo were examined. found that repression but not β1-AR selectively suppressed cell viability, induced G1-phase cycle...
Chondrosarcoma, a heterogeneous malignant bone tumor, commonly produces cartilage matrix, which generally has no response to conventional therapies. Studies have reported that MLN4924, NEDD8-activating enzyme inhibitor, achieves antitumor effects against numerous malignancies. In this study, the suppressive of MLN4924 on human chondrosarcoma cell lines were investigated using in vitro and vivo assays, involved measuring viability, cytotoxicity, apoptosis, proliferation, cycles,...
Exposure to particulate matter (PM) may contribute lung inflammation and injury. The therapeutic effect of N-acetylcysteine (NAC), a well-known antioxidant, with regards the prevention treatment fine PM (PM2.5)-induced injury is poorly understood. This study aimed determine PM2.5 on recruitment neutrophils Ly6Chigh monocytes into alveoli production proinflammatory proteins by stimulating generation reactive oxygen species (ROS), investigate NAC PM2.5-induced injury.C57BL/6 mice were exposed...
Background— Thrombomodulin is an anticoagulant, endothelial-cell-membrane glycoprotein. A recombinant thrombomodulin domain containing 6 epidermal growth factor–like structures exhibits mitogenic activity. This study explored the novel angiogenic effects of using in vitro and vivo models. Methods Results— Human (TMD2) TMD2 plus a serine threonine-rich (TMD23) were prepared Pichia pastoris expression system. Combined with purified or TMD23, thrombin effectively activated protein C. TMD23 had...
Abstract Cisplatin-based chemotherapy is the primary treatment for metastatic bladder urothelial carcinoma. However, response rate only 40–65%. This study investigated anti-tumor effect and underlying mechanisms of combination cisplatin NEDD8-activating enzyme inhibitor MLN4924 in human The exerted synergistic cytotoxicity on two high-grade carcinoma cell lines, NTUB1 T24 (combination index <1). also potentiated cisplatin-induced apoptosis activation caspase-3 -7, phospho-histone H2A.X...
Vascular calcification is the major reason for high mortality of cardiovascular complications diabetes. Interleukin (IL)-1β has been implicated in this pathogenesis, but its precise role and clinical evidence have not clearly identified. Hence, study was aimed to investigate whether concentration glucose (HG), which mimics hyperglycemia environment, could initiate vascular through NLRP3/IL-1β inflammasome underlying mechanism. Recently, 6-shogaol, a ginger derivate, elucidated pharmaceutic...
Abstract Fat embolism (FE) is a lethal medical emergency often caused by fracture of long bones and amputation limbs. Vascular endothelial growth factor (VEGF) promotes angiogenesis increases vascular permeability. We tested the hypothesis that VEGF plays critical role in FE-induced acute respiratory distress syndrome (ARDS) lung injury (ALI). tissues were collected from male Sprague-Dawley rats, animal oil was extracted mixed with water to form fatty micelles. The micelles then injected...
Cigarette use is an independent risk factor for the development of erectile dysfunction (ED). While association between chronic smoking and ED well established, fundamental mechanism(s) cigarette-related are incompletely understood, partly due to no reliable animal model smoking-induced ED. The present study was designed validate in vivo rat cigarette-induced Forty 12-week old male Sprague-Dawley rats were divided into 4 groups. Ten served as control group exposed only room air. remaining 30...
Oral squamous cell carcinoma (OSCC), an aggressive cancer originating in the oral cavity, is one of leading causes deaths males worldwide. This study investigated antitumor activity and mechanisms piperlongumine (PL), a natural compound isolated from Piper longum L., human OSCC cells. The effects PL on proliferation, cycle, apoptosis, senescence reactive oxygen species (ROS) levels cells were investigated. effectively inhibited growth, caused cycle arrest induced apoptosis Moreover,...
Evodiamine, an indole alkaloid derived from Evodia rutaecarpa, exhibits pharmacological activities including vasodilatation, analgesia, anti-cardiovascular disease, anti-Alzheimer's anti-inflammation, and anti-tumor activity.This study analyzes the effects of evodiamine on cellular growth, tumorigenesis, cell cycle apoptosis induction human urothelial carcinoma (UCC) cells.The present showed that significantly inhibited proliferation UCC cells in a dose- time-dependent manner. Also,...
No AccessJournal of UrologyAdult Urology1 Aug 2015The Efficacy Postoperative Adjuvant Chemotherapy for Patients with pT3N0M0 Upper Tract Urothelial Carcinoma Yun-Ching Huang, Miao-Fen Chen, Chung-Sheng Shi, Alan W. Shindel, Cih-En See-Tong Pang, Cheng-Keng Chuang, Chih-Shou Ying-Hsu Chang, Wei-Yu Lin, Dong-Ru Ho, Chih-Chien Chin, Yi-Hung Kuo, and Ching-Fang Wu HuangYun-Ching Huang Division Urology, Department Surgery, Chang Gung Memorial Hospital, Chiayi, Taiwan Graduate Institute Clinical...
The expression of thrombomodulin (TM), a calcium-dependent adhesion molecule, is frequently downregulated in various cancer types. However, the mechanism responsible for low level TM tumorigenesis unknown. Here, an inverse and Snail was detected different cell lines. We further confirmed this relation using epithelial-mesenchymal transition model HaCaT A431 cells. demonstrated that suppressed by binding to E-box (CACCTG) promoter. Moreover, knockdown short hairpin RNA disrupted...
Abstract The stromal cell‐derived factor‐1 (SDF‐1)/CXC receptor 4 (CXCR4) axis has been shown to play a role in colorectal cancer progression. In addition, the protease urokinase‐type plasminogen activator (uPA) is an important factor tumor cell invasion and metastasis. However, mechanism by which SDF‐1 mediates uPA expression human cells remains unknown. We investigated molecular governing interaction between stimulation three colon lines (DLD‐1, SW48, COLO 205). found that led increase...
Sepsis results from the host hyperinflammatory response to bacterial infection, causing multiple organ failure and high mortality. We previously demonstrated that LPS binds monocytic membrane-bound thrombomodulin (TM), but role of TM in LPS-induced inflammation remains unknown. In this study, we knockdown human cells attenuated signaling pathways cytokine production. Coimmunoprecipitation immunofluorescence assays showed interacted with receptors, CD14 TLR4/myeloid differentiation factor-2...
The N-terminal lectin-like domain (domain 1 [D1]) of thrombomodulin (TM) is known to have an anti-inflammatory function. We previously showed that recombinant TM (rTMD1) interacts with a carbohydrate molecule, Lewis Y (Le(y)), which found be expressed on adhesion molecules and involved in cell adhesion. Here, we tested the effect rTMD1/Le(y) interaction leukocyte recruitment inflammation.The expression Le(y) surface human umbilical vein endothelial cells was increased by tumor necrosis...
The CC chemokine receptor 6 (CCR6) and its ligand CCL20 are involved in human colorectal cancer (CRC) carcinogenesis can promote the progression of CRC. In addition, interleukin-17 (IL-17), produced by a T cell subset named "Th17," has been identified as an important player inflammatory responses, emerged mediator inflammation-associated cancer. However, relevance IL-17 development CRC still remains to be explored. This study aimed investigate effect on migration cells. Human HCT-116 cells...