A5104102056- Influenza Virus Research Studies
- Respiratory viral infections research
- Viral Infections and Vectors
- Orthopedic Surgery and Rehabilitation
- Knee injuries and reconstruction techniques
- interferon and immune responses
- Shoulder Injury and Treatment
- Tendon Structure and Treatment
- Mosquito-borne diseases and control
- Viral Infections and Immunology Research
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- T-cell and B-cell Immunology
- Elbow and Forearm Trauma Treatment
- RNA and protein synthesis mechanisms
- Immune Response and Inflammation
- Animal Disease Management and Epidemiology
- Periodontal Regeneration and Treatments
- Viral Infections and Outbreaks Research
- Vector-Borne Animal Diseases
- Peripheral Nerve Disorders
- Immunotherapy and Immune Responses
- Musculoskeletal synovial abnormalities and treatments
- Total Knee Arthroplasty Outcomes
- Lower Extremity Biomechanics and Pathologies
Cedars-Sinai Medical Center
2022-2025
The Graduate University for Advanced Studies, SOKENDAI
2024
Tokyo Medical and Dental University
2023-2024
University of Southern California
2024
The University of Tokyo
2024
Kagoshima Medical Center
2024
Institute for Molecular Science
2024
Shionogi (Japan)
2013-2023
Futaba (Japan)
2013-2023
Tokyo Medical and Dental University Hospital
2023
By searching the expressedsequence tag (EST) data base, we identified partial cDNA sequences encoding a novel human CC chemokine. We determined complete sequence that encodes highly basic polypeptide of total 98 amino acids with 20 to 30% identity other chemokines. termed this chemokine fromEBI1-Ligand Chemokine as ELC (see below). The mRNA was most strongly expressed in thymus and lymph nodes. Recombinant protein fusion Flag (ELC-Flag). For receptor-binding assays, recombinant fused...
Cap-dependent endonuclease (CEN) resides in the PA subunit of influenza virus and mediates critical "cap-snatching" step viral RNA transcription, which is considered to be a promising anti-influenza target. Here, we describe vitro characterization novel CEN inhibitor, baloxavir acid (BXA), active form marboxil (BXM). BXA inhibits transcription via selective inhibition activity enzymatic assays, replication infected cells without cytotoxicity cytopathic effect assays. The antiviral also...
Secondary Lymphoid-tissueChemokine (SLC) is a recently identified CC chemokine that constitutively expressed in various lymphoid tissues and potent specific chemoattractant for lymphocytes. The SLC gene the encoding another lymphocyte-specific chemokine, EBI1-ligand (ELC), form mini-cluster at human chromosome 9p13. Here, we show high affinity functional ligand receptor 7 (CCR7) on T B lymphocytes known ELC. induced vigorous calcium mobilization murine L1.2 cells stably expressing CCR7....
Secondary lymphoid tissue chemokine (SLC) is a CC expressed mainly in lymph nodes, appendix and spleen, specifically chemotactic for lymphocytes (Nagira et al., J. Biol. Chem. 1997. 272: 19518-19524). Here, we carried out transendothelial migration assays to determine the classes subsets of migrating toward SLC. SLC attracted freshly isolated B cells with high efficiency T modestly. Thus, first strong activity on fresh cells. Among cell types subsets, broadly CD4+ CD8+ cells, CD45RO- (naive)...
Abstract Tissue engineering is one new strategy being developed to treat ACL ruptures. One such approach bio‐enhanced repair, where a suture repair supplemented with bio‐active scaffold containing platelets. However, the optimal concentration of platelets stimulate healing not known. We hypothesized that increasing platelet concentrations in would enhance critical cell behaviors. Porcine fibroblasts were obtained from explant culture and suspended poor plasma (PPP), 1× platelet‐rich (PRP),...
Abstract The success of cell-based therapies to restore joint cartilage requires an optimal source reparative progenitor cells and tight control their differentiation into a permanent phenotype. Bone morphogenetic protein 2 (BMP-2) has been extensively shown promote mesenchymal cell chondrocytes in vitro vivo. Conversely, developmental studies have demonstrated decreased chondrocyte maturation by Wingless-Type MMTV Integration Site Family, Member 5A (Wnt5a). Thus, we hypothesized that...
Delayed anterior cruciate ligament (ACL) reconstruction often causes residual anterolateral rotatory instability (ALRI) and consequent knee osteoarthritis, warranting the recommendation of early ACL within 6 months after injury. Nonetheless, some cases show notable instability, even shortly The purpose this study was to identify risk factors for an increase in quantitatively measured pivot shift patients with ACL-deficient knees
Venipuncture is a commonly performed procedure to obtain blood for testing or donation, but nerve injuries with persistent chronic pain can occasionally occur. We implemented Phalen elbow flexion shoulder abduction (PEFSA) test screen potential donors who may be at risk after donation. Data were prospectively collected on all Red Cross centers in Kansai area of Japan 5 years prior PEFSA implementation, and then 2 implementation. Potential had positive given handout informing them potentially...
Collagen-platelet (PL)-rich plasma composites have shown in vivo potential to stimulate anterior cruciate ligament (ACL) healing at early time points large animal models. However, little is known about the cellular mechanisms by which component of these may healing. We hypothesized that components PL-rich (PRP), namely PLs and PL-poor (PPP), would independently significantly influence ACL cell viability metabolic activity, including collagen gene expression. To test this hypothesis, cells...
Abstract Use of platelet‐rich plasma (PRP) has shown promise in various orthopaedic applications, including treatment anterior cruciate ligament (ACL) injuries. However, components blood, peripheral blood mononuclear cells (PBMCs), are removed the process making PRP. It is yet unknown whether these PBMCs have a positive or negative effect on fibroblast behavior. To begin to define ACL fibroblasts, fibroblasts were cultured three‐dimensional collagen scaffolds for 14 days with and without...
Baloxavir marboxil (BXM) is an orally available small molecule inhibitor of cap-dependent endonuclease (CEN), essential enzyme in the initiation mRNA synthesis influenza viruses. In present study, we evaluated efficacy BXM against virus infection mouse models. Single-day oral administration completely prevented mortality due to with A and B mice. Moreover, 5-day repeated was more effective for reducing body weight loss mice infected than oseltamivir phosphate (OSP), even when treatment...
Purpose of this study: To elucidate the origin cell populations that contribute to rotator cuff healing, we developed a mouse surgical model where full-thickness, central detachment is created in supraspinatus.Three different inducible Cre transgenic mice with Ai9-tdTomato reporter expression (PRG4-9, αSMA-9, and AGC-9) were used label shoulder. The defect was surgically supraspinatus. injected tamoxifen at surgery cells sacrificed 1, 2, 5 weeks postoperatively. Frozen sections fluorescently...
Abstract Human infections with avian-origin influenza A(H7N9) virus represent a serious threat to global health; however, treatment options are limited. Here, we show the inhibitory effects of baloxavir acid (BXA) and its prodrug marboxil (BXM), first-in-class cap-dependent endonuclease inhibitor, against A(H7N9), in vitro vivo . In cell culture, BXA at four nanomolar concentration achieved 1.5–2.8 log reduction titers including NA-R292K mutant highly pathogenic avian viruses, whereas NA...
The medicinal chemistry and structure-activity relationships (SAR) for a novel series of carbamoyl pyridone bicycle (CAB) compounds as influenza Cap-dependent endonuclease (CEN) inhibitors are disclosed. Substituent effects were evaluated at the C (N)-1, N-3, C-7 positions CAB ring system using docking study. Submicromolar EC50 values achieved in cellular assay with C-7-unsubstituted which possessed benzhydryl group on either C-1 or N-1 position. An N-3 substituent was found to be critical...
Abstract Background The hemagglutinin (HA) of influenza viruses is a possible target for antiviral drugs because its key roles in the initiation infection. Although it was found that natural compound, Stachyflin, inhibited growth H1 and H2 but not H3 MDCK cells, inhibitory activity compound has been assessed against H4-H16 precise mechanism inhibition clarified. Methods Inhibitory Stachyflin viruses, as well H1-H3 examined cells. To identify factors responsible susceptibility to this...
Polybrominated diphenyl ethers (PBDEs) were measured in plastic resin pellets collected from 65 beaches across 27 countries worldwide. They detected at 49 locations concentrations of the sum congeners up to 46 ng/g-pellet and a median 2 ng/g-pellet. These values are one two orders magnitude lower than those polychlorinated biphenyls (PCBs) (median, 51 ng/g-pellet). This difference can be attributed production Penta-BDE technical mixture, which is used extensively some countries, availability...
Abstract Background Metacarpal shaft fractures are common and can be treated nonoperatively. Shortening, angulation, rotational deformity indications for surgical treatment. Various forms of treatment with advantages disadvantages have been documented. The purpose the study was to determine stability fracture fixation intramedullary headless compression screws in two types metacarpal compare them other rigid fixation: dorsal plating lag screw fixation. It hypothesized that would demonstrate...
Human infections caused by the H5 highly pathogenic avian influenza virus (HPAIV) sporadically threaten public health. The susceptibility of HPAIVs to baloxavir acid (BXA), a new class inhibitors for cap-dependent endonuclease, has been confirmed in vitro, but it not yet fully characterized. Here, efficacy BXA against HPAIVs, including recent H5N8 variants, was assessed vitro. antiviral marboxil (BXM) H5N1 virus-infected mice also investigated. exhibited similar vitro activities H5N1, H5N6,...
ABSTRACT We evaluated the efficacy of a single intravenous dose peramivir for treatment influenza B virus infection in ferrets and cynomolgus macaques present study. A (60 mg/kg body weight) given to on 1 day postinfection with significantly reduced median area under curve (AUC) titers (peramivir, 8.3 log 10 50% tissue culture infective doses [TCID 50 s]·day/ml; control, 10.7 TCID s·day/ml; P < 0.0001). Furthermore, nasal 2 receiving injection (30 mg/kg) AUCs temperature increase 60 were...
Ligand-based similarity screening of proprietary pharmaceutical company libraries enables rapid hit to lead investigation a chemotype with anti-<italic>leishmania</italic> activity.