A5043194092- Aquaculture disease management and microbiota
- Metabolism and Genetic Disorders
- Mitochondrial Function and Pathology
- Biochemical and Molecular Research
- Sperm and Testicular Function
- Influenza Virus Research Studies
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Reproductive Biology and Fertility
- HIV/AIDS drug development and treatment
- Muscle Physiology and Disorders
- Total Knee Arthroplasty Outcomes
- ATP Synthase and ATPases Research
- HIV Research and Treatment
- Pancreatic and Hepatic Oncology Research
- Microbial infections and disease research
- Aquaculture Nutrition and Growth
- Neonatal Health and Biochemistry
- Veterinary Medicine and Surgery
- Identification and Quantification in Food
- Respiratory viral infections research
- Reproductive Physiology in Livestock
- Cardiac Arrhythmias and Treatments
- Immune Response and Inflammation
- Virology and Viral Diseases
Nippon Veterinary and Life Science University
2014-2024
Matsumoto City Hospital
2019-2023
Takarazuka University of Medical and Health Care
2020-2022
Shionogi (Japan)
2008-2022
Yamagata University Hospital
2022
Tokyo Medical and Dental University Hospital
2022
Santen (Japan)
2012-2019
Iwate University
2019
Shinshu University Hospital
2019
Osaka University of Pharmaceutical Sciences
2019
It is found that a heartbeat period fluctuation usually has power spectral density which inversely proportional to frequency, spike added at breathing frequency.
We studied 40 MELAS patients (21 male and 19 female) to characterize the clinical features biochemical muscle biopsy findings related mtDNA mutation at nucleotide position of 3,243, most common genetic defect in MELAS. The frequent symptom was episodic sudden headache with vomiting convulsions, which commonly affected aged 5 15 years (80%). Biochemical defects were variable; 13 had complex I, seven IV, four complexes I + IV deficiencies. In biopsies without ragged-red fibers or any enzyme...
Abstract Objective Duchenne muscular dystrophy (DMD) is caused by the inability to produce dystrophin protein at myofiber membrane. A method rescue production antisense oligonucleotides, termed exon‐skipping, has been reported for mdx mouse and in four DMD patients local intramuscular injection. We sought test efficacy toxicity of intravenous oligonucleotide (morpholino)‐induced exon skipping dog model. Methods tested a series drugs singly as cocktails, both primary cell culture, two vivo...
S/GSK1349572 is a next-generation HIV integrase (IN) inhibitor designed to deliver potent antiviral activity with low-milligram once-daily dose requiring no pharmacokinetic (PK) booster. In addition, demonstrates against clinically relevant IN mutant viruses and has potential for high genetic barrier resistance. two-metal-binding strand transfer whose mechanism of action was established through in vitro enzyme assays, resistance passage experiments, viral strains resistant other classes...
Cap-dependent endonuclease (CEN) resides in the PA subunit of influenza virus and mediates critical "cap-snatching" step viral RNA transcription, which is considered to be a promising anti-influenza target. Here, we describe vitro characterization novel CEN inhibitor, baloxavir acid (BXA), active form marboxil (BXM). BXA inhibits transcription via selective inhibition activity enzymatic assays, replication infected cells without cytotoxicity cytopathic effect assays. The antiviral also...
The immunomodulatory effects of levamisole were investigated using rainbow trout, Oncorhynchus mykiss. Fish sampled 5 days after intraperitoneal injection showed increased protection against Vibrio anguillarum when a single dose (0.5 mg/kg) was used. Phagocytic activity, chemiluminescence responses and natural killer cell activity in fish treated with also increased, compared those the saline fish. Furthermore, activated alternative complement pathway although serum bactericidal activities...
Due to its excellent biocompatibility and mechanical properties, various different applications of polyvinyl alcohol-hydrogels (PVA-H) has been attempted in many fields. In the field orthopedic surgery, we have engaged for long time research on clinical PVA-H as a artificial cartilage, performed basic experiments synthesis PVA-H, developed implants using PVA-H. From these studies, articular intervertbral disc meniscus etc. developed. This review will present overview recent advances...
ABSTRACT GSK1265744 is a new HIV integrase strand transfer inhibitor (INSTI) engineered to deliver efficient antiviral activity with once-daily, low-milligram dose that does not require pharmacokinetic booster. The in vitro profile and mechanism of action were established through enzyme assays, resistance passage experiments, cellular assays site-directed molecular (SDM) clones resistant other classes anti-HIV-1 agents earlier INSTIs. inhibited replication low or subnanomolar efficacy...
This work is a continuation of our initial discovery potent monocyclic carbamoyl pyridone human immunodeficiency virus type-1 (HIV-1) integrase inhibitor that displayed favorable antiviral and pharmacokinetic properties. We report herein series bicyclic analogues to address conformational issues from SAR studies. modification the core unit succeeded deliver low nanomolar potency in standard assays. An additional hydroxyl substituent on scaffold provides remarkable improvement efficacies...
Abstract Thirteen of 15 patients with complex I deficiency had the multisystemic form, strokelike episodes and other symptoms that fulfilled diagnostic requirements for MELAS (mitochondrial myopathy, encephalopathy, lactic acidosis, episodes), 2 only muscle fatigability weakness, having purely myopathic form. In 12 ragged‐red fibers. All histochemical abnormalities consisted mild to moderate variation in fiber size (13), disorganized intermyofibrillar networks (12), type atrophy (10), an...
Abstract Bovine lactoferrin was evaluated for its ability to enhance resistance of rainbow trout, Oncorhynchus mykiss (Walbaum), infection with Vibrio anguillarum. Oral administration (100 mg kg −1 ) fish daily 3 days prior an intraperitoneal challenge V. anguillarum resulted in increased survival rates, and enhanced against Streptococcus sp., although a lesser extent. In lactoferrin‐treated fish, increase phagocytic chemiluminescent (CL) activities pronephros cells observed. The CL sp. were...
Abstract Enzymic activities of the respiratory chain and content immunochemically detectable subunits in NADH‐ubiquinone oxidoreductase (Complex I) were measured mitochondria from skeletal muscles 4 patients with mitochondrial myopathy, encephalopathy, lactic acidosis, strokelike episodes (MELAS). The rotenone‐sensitive NADH‐cytochrome c reductase activity was extremely decreased, ranging 0% to 27% control value. In all patients, Complex I also reduced parallel activity. It is suggested that...
Duchenne muscular dystrophy (DMD) is a severe and the most prevalent form of dystrophy, characterized by rapid progression muscle degeneration. Antisense-mediated exon skipping currently one promising therapeutic options for DMD. However, unmodified antisense oligos such as morpholinos require frequent (weekly or bi-weekly) injections. Recently, new generation vivo-morpholinos are reported to lead extensive prolonged dystrophin expression in dystrophic mdx mouse, an animal model The...
Drug-induced liver injury is a major problem in drug development and clinical therapy. In most cases the mechanisms are still unknown, thus, it difficult to predict or prevent these reactions. It has been known that halothane, an inhaled anesthetic, induces injury. To investigate of halothane-induced injury, we used recently established mouse model The expression transcription factors cytokines specific for Th1 Th2 (helper T cells), respectively, were compared between BALB/c C57BL/6 mice....
Abstract Cyclodextrins are commonly used as a safe excipient to enhance the solubility and bioavailability of hydrophobic pharmaceutical agents. Their efficacies mechanisms drug-delivery systems have been investigated for decades, but their immunological properties not examined. In this study, we reprofiled hydroxypropyl-β-cyclodextrin (HP-β-CD) vaccine adjuvant found that it acts potent unique adjuvant. HP-β-CD triggered innate immune response at injection site, was trapped by MARCO+...
Background/Aim: Wilms9 tumor 1 (WT1) is a tumor-associated antigen highly expressed in cancer. We examined the safety of WT1-peptide pulsed dendritic cell (WT1-DC) vaccine combination with chemotherapy patients surgically resected pancreatic Patients and Methods: Eight resectable cancer undergoing surgery either combined S-1 or plus gemcitabine therapy were enrolled. Immunohistochemical analysis WT1 was performed 34 cases Results: No serious side-effects observed, except grade I fever five...
Objective Smoking is associated with gastroesophageal reflux disease (GERD). Varenicline, a nicotinic receptor partial agonist, used to aid smoking cessation. The purpose of this study was prospectively examine the long-term benefits cessation on GERD and health-related quality life (HR-QOL). Methods Patients treated varenicline were asked fill out self-report questionnaire about their habits, gastrointestinal symptoms, HR-QOL before 1 year after prevalence GERD, frequency scores compared....
The naphthyridinone GSK364735 potently inhibited recombinant human immunodeficiency virus type 1 (HIV-1) integrase in a strand transfer assay (mean 50% inhibitory concentration +/- standard deviation, 8 2 nM). As expected based on the structure of drug, it bound competitively with another two-metal binding inhibitor (Kd [binding constant], 6 4 In number different cellular assays, HIV replication potency at nanomolar concentrations (e.g., peripheral blood mononuclear cells and MT-4 cells,...