A5061715662- Neonatal and fetal brain pathology
- Immune Cell Function and Interaction
- Neonatal Respiratory Health Research
- T-cell and B-cell Immunology
- Epilepsy research and treatment
- Mitochondrial Function and Pathology
- Neuroscience and Neuropharmacology Research
- RNA regulation and disease
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- EEG and Brain-Computer Interfaces
- Fetal and Pediatric Neurological Disorders
- Traumatic Brain Injury and Neurovascular Disturbances
- RNA and protein synthesis mechanisms
- Laser-Plasma Interactions and Diagnostics
- Intracerebral and Subarachnoid Hemorrhage Research
- Cholesterol and Lipid Metabolism
- Genetic Neurodegenerative Diseases
- MicroRNA in disease regulation
- Treatment of Major Depression
- Family Support in Illness
- Metabolism and Genetic Disorders
- Mental Health Research Topics
- Bacterial Infections and Vaccines
- Infectious Encephalopathies and Encephalitis
Takeda (Japan)
2013-2024
Nagoya City University
2004-2022
Jichi Medical University
2017
Kyoto University
2000-2012
National Autonomous University of Honduras
2008
VA Greater Los Angeles Healthcare System
2008
Kyoto University Institute for Chemical Research
2006-2008
University of California, San Francisco
2004-2007
Kyoto Pharmaceutical University
2006
Kurume University
2005
Based on the hypothesis that mitochondrial myopathy, encephalopathy, lactic acidosis, and strokelike episodes (MELAS) are caused by impaired vasodilation in an intracerebral artery, authors evaluated effects of administering l-arginine, a nitric oxide precursor. Patients were administered l-arginine intravenously at acute phase or orally interictal phase. l-Arginine infusions significantly improved all symptoms, suggesting oral administration within 30 minutes stroke decreased frequency...
The crp gene of E. coli, which codes for cAMP receptor protein (CRP), has been cloned in the plasmid pBR322 on basis a genetic complementation. One recombinant plasmids, pHA1, was shown to direct synthesis CRP cell-free system. location determined by constructing subclones carrying various portions pHA1. nucleotide sequence determined. coding region consists 627 base pairs (bp), specify 209 amino acids. predicted acid from DNA is consistent with partially known and composition CRP. After...
We have previously described how T and natural killer (NK) lineage commitment proceeds from common T/NK progenitors (p-T/NK) in the murine fetal thymus (FT), with use of a clonal assay system capable discriminating p-T/NK unipotent or NK lineage-committed (p-T p-NK, respectively). The molecular mechanisms controlling processes, however, are yet to be defined. In this study, we investigated progenitor activity FT cells Id2 −/− mice that exhibit defective cell development. FT, were greatly...
One hundred sixty seven survivors among very low birthweight infants with a gestational age of less than 35 weeks have been studied prospectively. The purpose this study was to clarify the relationship severe prenatal and perinatal complications hypocarbic alkalosis, defined as carbon dioxide tension (PaCO2) or equal 2.67 kPa pH 7.50 greater during first 24 hours life, cystic periventricular leukomalacia (PVL) depicted by serial cranial ultrasonographic examinations. Complications occurred...
We have established a new clonal assay system that can evenly support the development of T and natural killer (NK) cells. With this system, we show all cell progenitors in earliest CD44+CD25−FcγRII/III− fetal thymus (FT) population retain NK potential, lineage–committed (p-NK) also exist population. (p-T), which are unable to generate cells, first appear at CD44+CD25− FcγRII/III+ stage day 12 FT. The proportion p-T markedly increases during transition from CD44+CD25+ 14 On other hand, p-NK...
We report on GATA3 analysis and the phenotypic spectrum in nine Japanese families with HDR syndrome (hypoparathyroidism, sensorineural deafness, renal dysplasia) (MIM 146255). Fluorescence situ hybridisation microsatellite analyses showed heterozygous gross deletions including four families. Sequence novel mutations three families: a missense mutation within first zinc finger domain at exon 4 (T823A, W275R), an unusual (900insAA plus 901insCCT or C901AACCCT) resulting premature stop codon...
Flow cytometric and immunocytochemical analyses of murine fetal thymus (FT) cells with antibodies to various surface markers transcription factors reveal that the synthesis TCF-1 GATA-3 protein begins simultaneously in a fraction most immature population FT cells, which have phenotype CD4-CD8-CD44+CD25-. No TCF-1-producing is found liver (FL). In CD44+CD25- production immediately followed by intracellular expression CD3 epsilon. It also T cell development from FL, but not FT, progenitors...
Abstract Cholesterol 24-hydroxylase (CH24H) is a brain-specific enzyme that converts cholesterol into 24 S -hydroxycholesterol, the primary mechanism of catabolism in brain. The therapeutic potential CH24H activation has been extensively investigated, whereas effects inhibition remain poorly characterized. In this study, was investigated using newly identified small molecule, soticlestat (TAK-935/OV935). biodistribution and target engagement assessed mice. CH24H-knockout mice showed...
<b>Background: </b> Juvenile myoclonic epilepsy (JME) accounts for 3 to 12% of all epilepsies. In 2004, the GENESS Consortium demonstrated four missense mutations in <i>Myoclonin1/EFHC1</i> chromosome 6p12.1 segregating 20% Hispanic families with JME. <b>Objective: To examine what percentage consecutive JME clinic cases have <i>Myoclonin1/EFHC1</i>. <b>Methods: We screened 44 patients from Mexico and Honduras 67 Japan using heteroduplex analysis direct sequencing. <b>Results: found five...
The formation of 24S-hydroxycholesterol is a brain-specific mechanism cholesterol catabolism catalyzed by 24-hydroxylase (CYP46A1, also known as CH24H). CH24H has been implicated in various biological mechanisms, whereas pharmacological lowering not fully studied. Soticlestat novel small-molecule inhibitor CH24H. Its therapeutic potential was previously identified mouse model with an epileptic phenotype. In the present study, anticonvulsive property soticlestat characterized rodent models...
In juvenile myoclonic epilepsy, data are limited on the genetic basis of networks promoting convulsions with diffuse polyspikes electroencephalography (EEG) and subtle microscopic brain dysplasia called microdysgenesis.Using Sanger sequencing, we sequenced exomes six members a large family affected epilepsy confirmed cosegregation in all 37 members. We screened an additional 310 patients this disorder for variants DNA melting-curve analysis targeted real-time sequencing gene encoding...
The magnetic resonance findings in 22 children with athetotic cerebral palsy were studied. Sixteen had perinatal asphyxia, two neonatal jaundice, and four no association predisposing conditions. In six of the children, symmetrical high intensity areas found both thalamus putamen T2-weighted images. five they seen only thalamus, another one putamen. periventricular seen. seven subjects, abnormal Magnetic lesions, possibly caused by basal ganglia, and/or white matter 14 16 children. Three...
We investigated the developmental potential of hemopoietic progenitors in aorta-gonad-mesonephros (AGM) region, where definitive type have been shown to emerge before fetal liver develops. By using an assay system that is able determine individual toward T, B, and myeloid lineages, we show not only multipotent but also committed or lineage already exist this region day 10 fetuses. Bipotent generating T cells those B were detected, suggesting commitment cell lineages progress AGM region. The...
It has been unclear whether the progenitors colonizing thymus are multipotent or T cell lineage restricted. We investigated developmental potential of hematopoietic in various populations liver and blood cells from day 12 fetuses using recently established vitro experimental system effective determining capability individual to generate T, B, myeloid cells. Multipotent (p-Multi) were exclusively found Sca-1 high-positive (Sca-1high) subpopulation marker (Lin)-c-kit+CD45+ fetal Restriction...
In order to investigate the relationship between changes in glycosylation and placental materno-fetal transport of Immunoglobulin G (IgG), profiles IgG from healthy pregnant women their umbilical cords at delivery were compared by use a recently established high-performance liquid chromatography (HPLC) method. From results neutral oligosaccharide analysis, digalactosyl glycoform (G2) was markedly increased as with that age-matched controls. Furthermore, quantity cord found be higher than...
To estimate the risk of major depressive disorder (MDD) in adolescent and young adult (AYA) patients with cancer Japan identify factors for MDD among these patients.This was a matched cohort study using large claims database Japan. Included were aged 15-39 years, newly diagnosed during 2012-2017 assessable follow-up period 12 months. Kaplan-Meier estimates Cox proportional hazards models used to calculate hazard ratios (HR) 95% confidence intervals (CI) AYA versus age-, sex- working...
Several studies have noted modest interrater reliability of clinical electroencephalogram (EEG) interpretations. Moreover, no study to date has investigated a means improve the observed agreement. The purpose present was examine (i). EEG interpretations among three raters (two psychiatrists and one pediatrician); (ii). how by establishing consensus guideline for interpretation. Three raters, two pediatrician, interpreted 100 consecutive recorded at Tajimi General Hospital. After discussing...