A5078862494- Optical Coherence Tomography Applications
- Advanced Fluorescence Microscopy Techniques
- Digital Holography and Microscopy
- Cellular Mechanics and Interactions
- Optical measurement and interference techniques
- Cell Image Analysis Techniques
- 3D Printing in Biomedical Research
- Photoacoustic and Ultrasonic Imaging
- Angiogenesis and VEGF in Cancer
- Peptidase Inhibition and Analysis
- Neural and Behavioral Psychology Studies
- Ocular and Laser Science Research
- Signaling Pathways in Disease
- Transcranial Magnetic Stimulation Studies
- Advanced X-ray Imaging Techniques
- Congenital heart defects research
- Cardiac Arrhythmias and Treatments
- Cardiac Fibrosis and Remodeling
- Tissue Engineering and Regenerative Medicine
- Extracellular vesicles in disease
- Cancer Cells and Metastasis
- Muscle activation and electromyography studies
Korea University
2014-2023
The primed microenvironment of future metastatic sites, called the pre-metastatic niche, is a prerequisite for overt metastasis. However, mechanistic understanding contributions recruited cells to niche hindered by complex in vivo systems. Herein, microfluidic platform that incorporates endothelial and extracellular matrix (ECM) scaffolds developed, distinct role monocytes macrophages establishing niches delineated. It observed monocyte-derived metalloproteinase 9 facilitates cancer cell...
Field-based polarization measurements are essential for the completeness of information when exploiting complex nature optical responses target objects. Here, we demonstrate digital holographic microscopy quantifying a polarization-sensitive map an object with single-shot measurement. Using image-splitting device generating four different copies image and separate reference beam off-axis configuration enables multi-imaging capability. With use two filters, field images containing object's...
In the present study, we monitored hemodynamic responses in rat brains during transcranial direct current stimulation (tDCS) using functional near-infrared spectroscopy (fNIRS). Seven rats received anodal with 200 μA (DC) on their right barrel cortex for 10 min. The concentration changes of oxygenated hemoglobin (oxy-Hb) were continuously (10 min) and after (20 min). trend response was modeled linear regression, relationship between incremental decremental rates oxy-Hb investigated by...
Abstract This study aimed to develop a simulation model that accounts for skin-specific properties in order predict photothermal damage during skin laser treatment. To construct computational model, surface geometry information was obtained from an optical coherence tomography image, and the absorption coefficient of determined through spectrophotometry. The distribution internal light dose inside medium calculated using propagation based on Monte Carlo method. response due modeled by finite...
Mechanical interactions of living cells with the surrounding environment via focal adhesion (FA) in three dimensions (3-D) play a key role dynamic biological events, such as tissue regeneration, wound healing, and cancer invasion. Recently, several methods for observing 3-D cell–extracellular matrix (ECM) have been reported, lacking solid quantitative analysis on dynamics physical interaction between cell ECM. We measured submicron displacements ECM deformation due to protrusion-retraction...
Optical clearing has emerged as a powerful tool for volume imaging. Although imaging with immunostaining have been successful in many protocols, yet obtaining homogeneously stained thick samples remains challenging. Here, we propose method label-free of brain slices by enhancing the regional heterogeneity optical properties using tissue principle. We used FxClear, delipidation tissue, to retain larger proportion lipids at white matter (WM). Furthermore, embedding media affected contrasts...
Imaging three-dimensional (3-D) structures of biological specimens without exogenous contrast agents is desired in and medical science order not to disturb the physiological status living samples. Reflection phase microscopy based on interferometric detection has been useful for label-free observation such However, achievement optical sectioning mainly time gating set by broad spectra light sources. Here we propose wide-field reflection using a source narrow bandwidth, which yet capable...
Stromal cell-derived factor-1 alpha (SDF-1α, CXCL12) mediates the migration of circulating cells to desired sites for tissue development, homeostasis, and regeneration can be used promote cardiac by recruiting stem cells. However, use SDF-1α in injured heart necessitates not only higher binding affinity its receptor, CXCR4+, but also better robustness against enzymatic degradation than other SDF-1 isoforms. Here, we conduct a screening analog peptides that were designed structure-based drug...
The extracellular matrix (ECM) plays a key role during cell migration, proliferation, and differentiation by providing adhesion sites serving as physical scaffold. Elucidating the interaction between ECM can reveal underlying mechanisms of cellular behavior that are currently unclear. Analysis deformation due to cell-matrix interactions requires microscopic, three-dimensional (3-D) imaging methods, such confocal microscopy second-harmonic generation microscopy, which limited phototoxicity...
In article number 1900195, Seok Chung, Seung Hyeok Seok, and co-workers take the first step in providing direct evidence of sequential establishment pre-metastatic niche triggered by monocytes macrophages. The monocytes/macrophages-mediated remodeling endothelium-interstitial matrix leads down a slippery slope for circulating cancer cells to invade toward metastasis.
Biomedical researchers use optical coherence microscopy (OCM) for its high resolution in real-time label-free tomographic imaging. However, OCM lacks bioactivity-related functional contrast. We developed an system that can measure changes intracellular motility (indicating cellular process states) via pixel-wise calculations of intensity fluctuations from metabolic activity components. To reduce image noise, the source spectrum is split into five using Gaussian windows with 50% full...
Reflection phase microscopy is a valuable tool for acquiring three-dimensional (3D) images of objects due to its capability optical sectioning. The conventional method constructing 3D map capturing 2D at each depth with mechanical scanning finer than the This not only compromises sample stability but also slows down acquisition process, imposing limitations on practical applications. In this study, we utilized reflection microscope acquire locations significantly spaced apart, far beyond...