A5101611752- MicroRNA in disease regulation
- Adipose Tissue and Metabolism
- Circular RNAs in diseases
- Kruppel-like factors research
- Meat and Animal Product Quality
- Effects of Environmental Stressors on Livestock
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer-related gene regulation
- Peroxisome Proliferator-Activated Receptors
- Genetic Syndromes and Imprinting
- Lipid metabolism and biosynthesis
- Molecular Biology Techniques and Applications
- Fibroblast Growth Factor Research
- Cancer-related molecular mechanisms research
- Muscle Physiology and Disorders
- Research studies in Vietnam
- Migration, Aging, and Tourism Studies
- Immune Response and Inflammation
- Chemokine receptors and signaling
- Remote Sensing and Land Use
- Evaluation Methods in Various Fields
- Cholesterol and Lipid Metabolism
- RNA Research and Splicing
- RNA modifications and cancer
- Epigenetics and DNA Methylation
Southwest Minzu University
2018-2023
Simcere Pharmaceutical (China)
2023
Qinghai Tibetan Hospital
2020-2021
China Agricultural University
2021
State Ethnic Affairs Commission
2020
Guilin University of Technology
2018
Jiangxi Agricultural University
2012
Nantong Science and Technology Bureau
2011
Fibroblast growth factor 21 (FGF21) plays a critical role in the regulation of lipid metabolism; however, its function goat intramuscular fat (IMF) deposition remains unknown. To explore FGF21 for IMF deposition, we performed gain and loss adipocyte. Our results showed that overexpression mediated by adenovirus inhibits accumulation adipocyte, accompanied down-regulating mRNA levels peroxisome proliferator activated receptor γ (PPARγ), adipocyte fatty acid-binding protein 2 (aP2) sterol...
Abstract Background Intramuscular adipocytes differentiation is a complex process, which regulated by various transcription factor, protein factor regulators and signal transduction pathways. However, the proteins pathways that regulates goat intramuscular remains unclear. Result In this study, based on nanoscale liquid chromatography mass spectrometry analysis (LC-MS/MS), tandem tag (TMT) labeling was used to investigate differentially abundant (DAPs) related with process of adipocytes....
Intramuscular fat (IMF) deposition is one of the most important factors to affect meat quality in livestock and induce insulin resistance adverse metabolic phenotypes for humans. However, key regulators involved this process remain largely unknown. Although liver kinase B1 (LKB1) was reported participate development skeletal muscles classical adipose tissues. Due specific autonomic location intramuscular adipocytes, deposited between or within muscle bundles, exact roles LKB1 IMF need...
Goat intramuscular fat (IMF) content is mainly determined by the processes of preadipocytes adipogenic differentiation and mature adipocyte lipid accumulation. However, underlying regulators these biological remain largely unknown. Here, we report that expression Liver X receptor alpha (LXRα) reaches a peak at early stage then gradually decreases during goat adipogenesis. Knockdown LXRα mediated two independent siRNAs significantly inhibits adipocytes accumulation upregulates marker-...
Abstract KLF9 is reported to promote adipocyte differentiation in 3T3-L1 cells and pigs. However, the roles of adipocytes goat remain unknown. In this study, expression profiles were different between subcutaneous intramuscular preadipocytes during process. After silencing gene, lipid droplets increased both two types adipocytes. preadipocyte with KLF9, expressions C/EBPβ, PPARγ, LPL, KLF1-2, KLF5, KLF17 genes up-regulated, while KLF12, KLF4, KLF13 down-regulated level. preadipocyte, aP2,...
GTP binding protein overexpressed in skeletal muscle (GEM) is an important gene with many functions, such as regulating the rearrangement of cytoskeleton and activity voltage-dependent calcium channel, GEM was regarded a candidate for obesity. However, little investigation has been carried out to explore whether affected intramuscular fat (IMF) deposition goat. To role goat, this cloned its tissue temporal expression profile were detected. Effect on adipogenesis examined by losing function...
Abstract Previous research reported that KLF 3 plays different roles in the regulation of adipose deposition across species. However, exact function goat subcutaneous adipocyte remains unknown. Here, KLF3 gene was firstly cloned and showed mRNA sequence 1,264 bp (GenBank accession number: KU041753.1) its coding 1,037 bp, encoding 345 amino acids with three classic zinc finger domains KLFs family at C‐terminus. The alignment acid among various species demonstrated had highest homology to...
Intramuscular fat (IMF) deposition is a complicated process, and most of the underlying regulators this biological process are unknown. Here, we cloned intact CDS KLF4 gene, investigated role by gaining or losing function in vitro further explored pathways regulating differentiation intramuscular preadipocytes goat. Our results show that goat gene consists 1,536 bp encoding protein 486 amino acids. The expression higher lung while lower heart muscle Knockdown mediated siRNA technique...
Intramuscular fat (IMF) content determined by the intramuscular preadipocytes differentiation has a huge influence on sensory quality traits of meats. It was reported that adiponectin (ADIPOQ) gene could promote adipocytes differentiation, but underlying molecular and functional characterization ADIPOQ for regulating goat IMF deposition remained unknown. Herein, knockdown mediated siRNAs during differentiation. Also, qRT-PCR technique performed to detect mRNA levels target genes in multiply...
Intramuscular fat contributes to the improvement of meat quality goats. MicroRNAs (miRNAs) have been reported regulate adipocyte differentiation and maturation. The aim our study was clarify whether miR-10a-5p regulates goat intramuscular preadipocyte (GIPC) its direct downstream signaling pathway. GIPCs were isolated from longissimus dorsi, whose level measured at different time point induction. Adipogenic evaluated by Oil Red O BODIPY staining, expression changes adipogenic genes like ACC,...
Resistin (RETN) is a hormone secreted by adipocytes, which plays an important role in glucose and lipid metabolism. The aim of this study to clone obtain the full length open reading frame (ORF) goat RETN gene sequence, reveal its molecular expression characteristics. Simultaneously, we explore effect on differentiation intramuscular preadipocytes goat. ORF sequence was cloned RT-PCR technique, bioinformatics analysis performed though relevant biological softwares. In study, mRNA tissues...
Abstract Background The procession of preadipocytes differentiation into mature adipocytes involves multiple cellular and signal transduction pathways. Recently. a seirces noncoding RNAs (ncRNAs), including circular (circRNAs) were proved to play important roles in regulating adipocytes. Result In this study, we aimed identificate the potential circRNAs early late stages goat intramuscular differentiation. Using bioinformatics methods predict their biological functions map circRNA-miRNA...
Retinoid X receptor α (RXRα) is thought to be a key regulator in lipid metabolism, glucose adipogenic differentiation, gene expression, and inflammatory response. However, it not clear whether RXRα has any role intramuscular preadipocyte of goat. In the current investigation, we report that adenovirus overexpression promotes accumulation adipocyte up-regulates expression positive indicator genes (PPARγ GLUT4). Next, knockdown does affect adipogenesis adipocyte. This phenomenon may caused by...
Reverse transcription quantitative real-time PCR (RT-qPCR) is a common and high-efficiency technique for detecting the mRNA levels of genes where suitable reference were introduced to normalize data RT-qPCR. However, little known about in adipose tissue Jianzhou da'er goat. Here, six housekeeping genes, including ACTB, ALAS1, EIF3K, HSP90, RPLP0, UXT, selected as candidate while FASN, HSL, LPL, PID1 used target validation suitability identified genes. After expression geNorm, NormFinder,...
KLF7 belongs to the Krüppel-like factors (KLFs) family, which function as transcriptional regulators controlling a number of basic cellular processes, involving proliferation, differentiation, and migration. Here, we reveal insights into differentiated expression in different goat tissues stages growth, inhibition role knockdown differentiation by using intramuscular subcutaneous preadipocytes. We demonstrate that is obviously changed during preadipocytes mature adipocytes. Knockdown...
Abstract Identifying fusion genes in solid tumors is crucial for precision diagnosis and treatment of cancer patients. However, poor RNA quality may pose a major challenge to the reliability detection. In this study, an optimized detection method using targeted next-generation sequencing was developed validated detect gene fusions formalin-fixed, paraffin-embedded (FFPE) samples, where standard DV 200 as low 20%. Uniquely designed probes that target junction sequences enhances realism...