A5037288975- PARP inhibition in cancer therapy
- Cell death mechanisms and regulation
- DNA Repair Mechanisms
- Cancer-related molecular mechanisms research
- Sirtuins and Resveratrol in Medicine
- Circular RNAs in diseases
- Corneal Surgery and Treatments
- Carcinogens and Genotoxicity Assessment
- Mycotoxins in Agriculture and Food
- MicroRNA in disease regulation
- ATP Synthase and ATPases Research
- Cancer, Hypoxia, and Metabolism
- Synthesis and biological activity
- RNA modifications and cancer
- Genomics, phytochemicals, and oxidative stress
- Protein Tyrosine Phosphatases
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced Biosensing Techniques and Applications
- RNA and protein synthesis mechanisms
- Autophagy in Disease and Therapy
- Bacillus and Francisella bacterial research
- Analytical Chemistry and Chromatography
- CAR-T cell therapy research
- Anodic Oxide Films and Nanostructures
- Signaling Pathways in Disease
Guangzhou Blood Center
2024-2025
Guangzhou Medical University
2025
Guangdong Medical College
2012-2023
Lanzhou University of Technology
2021-2022
Dongguan University of Technology
2017
University of Benin
2016
Inserm
2005-2007
Centre de Recherche des Cordeliers
2005-2007
Université Paris Cité
2007
Sun Yat-sen University
2005
ABSTRACT The proliferation of CAR‐T cells was hindered and cannot play its killing function well in solid tumors. And yet the regulatory mechanism cell is not fully understood. Here, we showed that recombinant expression CD19CAR T significantly increased basal activation level LCK activation. Both SMAD4 were essential for since over‐express or promotes proliferation, while knock‐down inhibited seriously. More go into apoptosis when expression, cycle arrested G2/M S phase, respectively....
Cervical cancer is a common gynecological malignancy with high incidence and mortality. Drugs commonly used in chemotherapy are often accompanied by strong side-effects. To find an anti-cervical drug effects low toxicity, luteoloside was to treat the cervical cell line Hela investigate its on morphology, proliferation, apoptosis, related proteins. The study demonstrated that could inhibit proliferation remarkably; promote apoptosis cytochrome C release; decrease mitochondrial membrane...
DPPH• scavenging peptides (<3kDa) from underutilized Dunaliella salina protein were obtained by the following successive treatment, i.e., ultrasound extraction, simulated in vitro gastrointestinal digestion hydrolyzation, and membrane ultrafiltration classification. The optimal condition for ultrasound-assisted extraction was an wave with 800 W of power treating a mixture 60 mL 1.0 mol L-1 NaOH 2 g algae powder 15 min. A high correlation (r=0.8146) between activity yield intact showed their...
The gene V protein of bacteriophage f1 is a single-stranded DNA and RNA-binding composed two identical subunits. We have constructed single-chain variants the using short peptide linkers five or six amino acids to connect carboxyl terminus one monomer second monomer. resulting subunit-fusion proteins were found bind nearly as tightly wild-type protein. Denaturation measurements show that are 5 kcal/mol (1 kcal = 4.18 kJ) more stable than at concentration 10 microM. rate unfolding essentially...
The ets family of eukaryotic transcription factors is characterized by a conserved DNA-binding domain approximately 85 amino acids for which the three-dimensional structure not known. By using multidimensional NMR spectroscopy, we have determined secondary one member this gene family, human Fli-1, both in free form and complex with 16-bp cognate DNA site. Fli-1 consists three alpha-helices short four-stranded antiparallel beta-sheet. This arrangement resembles that catabolite activator...
B cell leukemia/lymphoma‐2 (Bcl‐2) suppresses apoptosis by binding the BH3 domain of proapoptotic factors and thereby regulating mitochondrial membrane potential (MMP). This study aimed to investigate role Bcl‐2 in controlling pathway during hydroquinone (HQ)‐induced TK6 cytotoxicity. In this study, HQ, one metabolite benzene, decreased MMP a concentration‐dependent manner induced generation reactive oxygen species (ROS), activation DNA damage marker γ‐H2AX, production damage‐responsive...
Reprogramming of cellular metabolism is a vital event during tumorigenesis. The role glycolysis in malignant progression promoted by hydroquinone (HQ), one the metabolic products benzene, remains to be understood. Recently, we reported overexpression sirtuin 1 (SIRT1) HQ-enhanced TK6 cells and hypothesized that SIRT1 might contribute favor Our data showed acute exposure HQ for 48 h inhibited glycolysis, as indicated reduction glucose consumption, lactate production, hexokinase activity,...
Hydroquinone (HQ), one of the most important metabolites derived from benzene, induces cell cycle arrest and apoptosis. Poly(ADP-ribose) polymerase-1 (PARP-1) participates in various biological processes, including DNA repair regulation. To explore whether PARP-1 regulatory pathway mediated HQ-induced apoptosis, we assessed effect suppression on induction apoptosis analyzed by FACSCalibur flow cytometer deficientTK6 cells (TK6-shPARP-1). We observed an increase fraction G1 phase 7.6%...
Dichloroacetonitrile (DCAN), one of the disinfection byproducts water chlorination, induces cell proliferation and apoptosis; however, detailed mechanism remains unclear. Oxidative stress participates in various biological processes, including DNA damage cytotoxicity. To explore whether oxidative mediated DCAN-induced apoptosis, we assessed effect redox imbalance apoptosis LO2 cells. We observed increase reactive oxygen species malondialdehyde increased by 13.6% 500 μM DCAN compared with...
Hydroquinone (HQ), a major reactive metabolite of benzene, contributes to benzene‑induced leukemia. The molecular mechanisms that underlie this activity remain be elucidated. Poly ADP‑ribosylation (PARylation) is type reversible posttranslational modification performed by enzymes in the PAR polymerase (PARP) family and mediates different biological processes, including apoptosis. Zona occludens 2 (ZO‑2) tight junction scaffold protein, which involved cell proliferation present study...
Long non-coding RNAs (lncRNAs) have been shown to play a critical role in the damage caused body by environmental exogenous chemicals; however, few studies explored their effects during exposure benzene and its metabolite, hydroquinone (HQ). An emerging lncRNA, LINC01480, was found be associated with immune microenvironment of some cancers, but specific function remains unknown. Therefore, this study aimed investigate LINC01480 HQ-induced apoptosis. The biological investigated through...
// Shaoyun Chen 1, * , Hairong Liang Gonghua Hu 2, Hui Yang 1 Kairu Zhou Longmei Xu Jiaxian Liu Bei Lai Li Song Hao Luo Jianming Peng 3 Zhidong Yongmei Xiao 4 Wen and Huanwen Tang Department of Environmental Occupational Health, Dongguan Key Laboratory Medicine, School Public Guangdong Medical University, Dongguan, 523808, China 2 Preventive Gannan Ganzhou, 341000, Huizhou Prevention Treatment Centre for Disease, Huizhou, 516000, Guangzhou Pollution Health Risk Assessment, Toxicology, Sun...
Hydroquinone (HQ), a key metabolite of benzene, affects cell cycle and apoptosis. Poly (ADP-ribose) polymerase-1 (PARP-1) plays an important role in DNA damage repair. To explore whether PARP-1 is involved HQ-induced apoptosis, we assessed the effect suppression overexpression on induction apoptosis analyzed by flow cytometry analysis. We observed that HQ induced aberrant progression further confirmed accelerated inhibited via inhibiting p16/pRb signal pathway after acute exposure, while...
The p16INK4A is a multifunction gene that includes regulation of the cell cycle, apoptosis, senescence and tumor development. However, effects p16 in hydroquinone-induced malignant transformation TK6 cells remain unclear. present study aimed to explore whether loss facilitate cells. results demonstrated p16/Rb signal pathway was suppressed We further confirmed stimulated proliferation, accelerated cycle progression vitro vivo. immunoblotting analysis indicated regulated via Rb p53....
4110 Background: In inoperable pancreatic cancer, many randomized controlled trials documented that gemcitabine-based chemotherapy had higher objective remission rate, but no more survival than gemcitabine alone. Methods: MEDLINE and EMBASE searches were supplemented by information from trial registers. Randomized for combination alone in cancer(from 1996 to 2004). A quantitative meta-analysis using updated basing on inclusion criterion all available was carried out two reviewers. The...
Purpose: To investigate the effects of luteoloside on proliferation human chronic myeloid leukemia K562 cells and whether induces cell cycle arrest apoptosis in cells.Methods: Luteoloside's cytotoxicity was assessed using a counting kit. Cell distribution analysed by flow cytometry after propidium iodide (PI) staining. assayed with detection kit Hoechst staining followed observation under fluorescence microscope. The expression cycle- apoptosis-related proteins examined Western blot...