A5038709593- Protein Structure and Dynamics
- Genetic Neurodegenerative Diseases
- Mitochondrial Function and Pathology
- Enzyme Structure and Function
- Proteins in Food Systems
- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Mass Spectrometry Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Protein Interaction Studies and Fluorescence Analysis
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- Electrochemical sensors and biosensors
- Photosynthetic Processes and Mechanisms
- RNA Research and Splicing
- Blood groups and transfusion
- Analytical Chemistry and Chromatography
- Cancer Genomics and Diagnostics
- Alzheimer's disease research and treatments
- Ubiquitin and proteasome pathways
- Neurobiology and Insect Physiology Research
- Fungal and yeast genetics research
- Animal health and immunology
Novo Nordisk (Denmark)
2022-2024
Novo Nordisk (Switzerland)
2023
Novo Nordisk (United Kingdom)
2023
University of Copenhagen
2014-2021
University of Milano-Bicocca
2004-2015
University of Milan
2008-2009
In the last years, a growing interest has been gathering around ability of Molecular Dynamics (MD) to provide insight into paths long-range structural communication in biomolecules. The knowledge mechanisms related helps rationalization atomistic details effects induced by mutations, ligand binding, and intrinsic dynamics proteins. We here present PyInteraph, tool for analysis ensembles inspired graph theory. PyInteraph is software suite designed analyze MD with attention binary interactions...
The risk of inducing hypoglycaemia (low blood glucose) constitutes the main challenge associated with insulin therapy for diabetes
Abstract Electrochemical gradients are essential to the functioning of cells and typically formed across membranes using active transporters require energy input maintain them. Here, we show by contrast that biomolecular condensates able sustain significant pH without any external input. We explore thermodynamic driving forces establish this gradient a microfluidics-based droplet platform allows us sample in continuous manner both stability composition wide range. These results reveal...
The protein ataxin-3 consists of an N-terminal globular Josephin domain (JD) and unstructured C-terminal region containing a stretch consecutive glutamines that triggers the neurodegenerative disorder spinocerebellar ataxia type 3, when it is expanded beyond critical threshold. disease results from misfolding aggregation, although pathway structure aggregation intermediates are not fully understood. In order to provide insight into mechanism process, we monitored normal (AT3Q24) ataxin-3,...
Nonspecific interactions are a key challenge in the successful development of therapeutic antibodies. The tendency for nonspecific binding antibodies is often difficult to reduce by rational design, and instead, it necessary rely on comprehensive screening campaigns. To address this issue, we performed systematic analysis impact surface patch properties antibody nonspecificity using designer library as model system single-stranded DNA ligand. Using an in-solution microfluidic approach, find...
ARID is a DNA-binding domain involved in several transcriptional regulatory processes, including cell-cycle regulation and embryonic development. domains are also targets of the Human Cancer Protein Interaction Network. Little known about molecular mechanisms related to conformational changes family domains. Thus, we have examined their structural dynamics enrich knowledge on this important proteins. In particular, used an approach that integrates atomistic simulations methods inspired by...
Molecular dynamics (MD) simulations are widely used to complement or guide experimental studies in the characterization of protein dynamics, thanks improvements force-field accuracy, along with software and hardware sample conformational landscape proteins. Among different applications MD simulations, study correlated motions is largely employed for purposes. Several metrics have been developed describe ensemble, such as methods based on Pearson Correlation Mutual Information....
Abstract Nano‐electrospray‐ionization mass spectrometry (nano‐ESI‐MS) is applied to comparison of bovine and porcine β‐lactoglobulin (BLG PLG). The conformational oligomeric properties the two proteins under different solvent experimental conditions are analyzed. pH‐dependence dimerization described for pH range 2–11. results indicate maximal dimer accumulation at 6 BLG 4 PLG, as well a lower stability PLG compared 6. Conformational appears be higher acidic pH, but basic pH. low revealed by...
Several neurodegenerative diseases are triggered by proteins containing a polyglutamine (polyQ) stretch expanded beyond critical threshold. Among these, ataxin-3 (AT3) is the causative agent of spinocerebellar ataxia type-3. We expressed three authentic AT3 variants in Escherichia coli: one normal (AT3-Q24), (AT3-Q55) and truncated immediately upstream polyQ (AT3-291Δ). Then, based on growth rate reduction, we quantified protein toxicity. show that AT3-Q55 -291Δ strongly reduced early stages...
Aggregation of human ataxin-3 (AT3) into amyloid fibrils is responsible for spinocerebellar ataxia type 3. This protein consists a folded N-terminal domain (Josephin domain, residues 1-182), central flexible region (residues 183-291), poly-glutamine sequence variable length and short C-terminal region. Very little known about the influence on conformational aggregation properties this protein. The present study aimed to investigate specific role portion 183-291). Accordingly, fragments 1-182...
Ubiquitin is a small protein at the heart of many cellular processes, and several different domains are known to recognize bind ubiquitin. A common motif for interaction with ubiquitin Interacting Motif (UIM), characterized by conserved sequence signature often found in multi-domain proteins. Multi-domain proteins intrinsically disordered regions mediate interactions multiple partners, orchestrating diverse pathways. Short linear motifs binding embedded these play crucial roles modulating...