A5033153121- Hepatitis B Virus Studies
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- interferon and immune responses
- Pancreatic and Hepatic Oncology Research
- HIV/AIDS drug development and treatment
- Hepatitis Viruses Studies and Epidemiology
- Immune Response and Inflammation
- Viral gastroenteritis research and epidemiology
- Immunotherapy and Immune Responses
- Influenza Virus Research Studies
- Endoplasmic Reticulum Stress and Disease
- Drug-Induced Hepatotoxicity and Protection
- Ginger and Zingiberaceae research
- Liver physiology and pathology
- Plant biochemistry and biosynthesis
- Biochemical and biochemical processes
Sungkyunkwan University
2021-2024
Konkuk University
2017-2024
Korea Research Institute of Bioscience and Biotechnology
2021
Chungbuk National University
2021
Objective Interferons (IFNs) mediate direct antiviral activity. They play a crucial role in the early host immune response against viral infections. However, IFN therapy for HBV infection is less effective than other Design We explored cellular targets of to IFNs using proteome-wide screening. Results Using LC-MS/MS, we identified proteins downregulated and upregulated by treatment X protein (HBx)-stable control cells. found several IFN-stimulated genes HBx, including TRIM22 , which known as...
Background/Aims: The major histocompatibility class II (MHC II) transactivator, known as CIITA, is induced by Interferon gamma (IFN-γ) and plays a well-established role in regulating the expression of MHC molecules antigen-presenting cells.Methods: Primary human hepatocytes (PHH) were isolated via therapeutic hepatectomy from two donors. hepatocellular carcinoma (HCC) cell lines HepG2 Huh7 used for mechanistic study, HBV infection was performed HepG2-NTCP cells. DNA replication intermediates...
The antiviral role of the tripartite motif-containing (TRIM) protein family , a member E3-ubiquitin ligase family, has recently been actively studied. Hepatitis B virus (HBV) infection is major contributor to liver diseases; however, host factors regulated by cytokine-inducible TRIM21 suppress HBV remain unclear. In this study, we showed efficacy against in hepatoma cell lines, primary human hepatocytes isolated from patient tissues, and mouse model. Using knock-out cells, confirmed that...
Hepatitis B virus (HBV) infection is a leading cause of liver diseases; however, the host factors which facilitate replication and persistence HBV are largely unidentified. Cellular FLICE inhibitory protein (c-FLIP) typical antiapoptotic protein. In many cases diseases, expression level c-FLIP altered, affects fate hepatocytes. We previously found that its cleaved form interact with X (HBx), essential for replication, regulate diverse cellular signals. this study, we investigated role...
Hepatitis B virus (HBV) infection is a major factor in the development of various liver diseases such as hepatocellular carcinoma (HCC). Among HBV encoded proteins, X protein (HBx) known to play key role HCC. Hepatocyte nuclear 4α (HNF4α) transcription which critical for hepatocyte differentiation. However, expression level well its regulatory mechanism have yet be clarified. Here, we observed suppression HNF4α cells stably express whole genome or HBx alone, while transient transfection...
Tenofovir disoproxil fumarate (TDF) has been regarded as the most potent drug for treating patients with chronic hepatitis B (CHB). However recently, viral mutations associated tenofovir have reported. Here, we found a CHB patient suboptimal response after more than 4 years of TDF treatment. Clonal analysis virus (HBV) isolated from sequential sera this identified seven previously reported TDF-resistant (CYELMVI). Interestingly, threonine to alanine mutation at 301 amino acid position...
Currently, interferon alpha and nucleos(t)ide analogues (NAs) are clinically available to treat hepatitis B virus (HBV) infection. Several NAs, including lamivudine (LMV), adefovir (ADV), entecavir (ETV) tenofovir (TDF or TAF) have been approved administered chronic (CHB) patients. NAs inhibit HBV DNA synthesis by targeting the reverse transcriptase (RT) domain of polymerase. mutations in RT which lead drug resistance against reported, even for TDF TAF highly potent with very low rate....
Hepatitis B virus (HBV) is a sex-specific pathogen that more severe in males than females. Sex disparities HBV infection have been attributed to hormonal differences between and However, whether affects the metabolic signatures of steroid hormones how these influences viral replication remains unclear. In this study, we investigated alters metabolism its effects on replication. Serum samples from male female mice obtained after hydrodynamic injection replication-competent plasmids were...
Hepatitis B virus (HBV) can cause chronic infections, significantly increasing the risk of death from cirrhosis and hepatocellular carcinoma (HCC). A key player in HBV infection is covalently closed circular DNA (cccDNA), a stable episomal form viral that acts as persistent reservoir infected hepatocytes drives continuous replication. Despite development several animal models, few adequately replicate cccDNA formation maintenance, limiting our understanding its dynamics evaluation potential...
Zingerone (vanillylacetone; 4-hydroxy-3-methoxyphenylethyl methyl ketone) is a key component responsible for the pungency of ginger (Zingiber officinale). In this study, it was confirmed that type III polyketide synthase (PKS) gene (pmpks) from Piper methysticum exhibits feruloyl-CoA-preferred benzalacetone (BAS) activity. Based on these results, we constructed an artificial biosynthetic pathway zingerone production supplemented ferulic acid with 4-coumarate CoA ligase (4CL), PmPKS, and...
Hepatitis B virus (HBV) is known to cause severe liver diseases such as acute or chronic hepatitis, cirrhosis and hepatocellular carcinoma. Chronic hepatitis (CHB) infection a major health problem with nearly 300 million individuals infected worldwide. Currently, nucleos(t)ide analogs (NAs) interferon alpha are clinically approved treatments for HBV infection. NAs potent antiviral agents that bind polymerase block viral reverse transcription replication. Besifovir dipivoxil maleate (BSV)...
Hepatitis B virus (HBV) infection is a major factor in development of various liver diseases such as hepatocellular carcinoma (HCC). Among HBV encoded proteins, X protein (HBx) known to play key role HCC. Hepatocyte nuclear 4α (HNF4α) transcription which critical for hepatocyte differentiation. However, the expression level well its regulatory mechanism have yet be clarified. Here, we observed suppression HNF4α cells stably express whole genome or HBx alone,...
Hepatitis B virus (HBV) is known to cause severe liver diseases such as acute or chronic hepatitis, cirrhosis and hepatocellular carcinoma. Chronic hepatitis (CHB) infection a major health problem with nearly 300 million individuals infected worldwide. Currently, nucleos(t)ide analogs (NAs) interferon alpha are clinically approved treatments for HBV infection. NAs potent antiviral agents that bind polymerase block viral reverse transcription replication. Besifovir dipivoxil maleate (BSV)...