A5074896508- Periodontal Regeneration and Treatments
- Osteoarthritis Treatment and Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Hemostasis and retained surgical items
- Proteoglycans and glycosaminoglycans research
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Inflammatory mediators and NSAID effects
- Oral and gingival health research
- Bone and Joint Diseases
- Spectroscopy Techniques in Biomedical and Chemical Research
- Sphingolipid Metabolism and Signaling
- Erythrocyte Function and Pathophysiology
- Genital Health and Disease
- Blood transfusion and management
- Protease and Inhibitor Mechanisms
- Tendon Structure and Treatment
- Knee injuries and reconstruction techniques
Semmelweis University
2018-2022
Universitätsklinikum Krems
2021
Platelet-rich fibrin (PRF) membrane is a three-dimensional biodegradable biopolymer, which consists of platelet derived growth factors enhancing cell adhesion and proliferation. It widely used in soft hard tissue regeneration, however, there are unresolved problems with its clinical application. Its preparation needs open handling the membranes, it degrades easily, has low tensile strength does not hold suture blocking wider applications PRF. Our aim was to produce sterile, suturable,...
Autologous blood derived products, such as platelet-rich plasma (PRP) and fibrin (PRF) are widely applied in regenerative therapies, contrast to the drawbacks their application, mainly deriving from preparation methods used. Eliminating disadvantages of both PRP PRF, hyperacute serum (HAS) opens a new path autologous therapy showing similar or even improved potential at same time. Despite frequent experimental clinical use HAS, protein composition has not been examined thoroughly yet. Thus,...
One option to fight joint degradation and inflammation in osteoarthritis is the injection of activated blood products into synovial space. It has been demonstrated that hyperacute serum most proliferative among plasma products, so we investigated how cytokine milieu osteoarthritic knee reacts treatment vitro. Cartilage, subchondral bone, membrane explanted from knees were stimulated by interleukin-1 beta (IL-1β) concentration 39 biomarkers was measured co-culture supernatant after treatment....
Mesenchymal stem cells (MSCs) are widely used in laboratory experiments as well human cell therapy. Their culture requires animal sera like fetal calf serum (FCS) essential supplementation; however, pose a risk for clinical applications. Human blood derivatives, example, platelet-rich plasma (PRP) releasates, potential replacements of FCS; it is unclear which variant has the best effect on given or tissue type. Additionally, derivatives commonly musculoskeletal diseases osteoarthritis (OA)...
Hyaluronic acid (HA) is an ideal initial material for preparing hydrogels, which may be used as scaffolds in soft tissue engineering based on their advantageous physical and biological properties. In this study, two crosslinking agents, divinyl sulfone (DVS) butanediol diglycidyl ether, were to investigate effect the properties of HA hydrogels. As hydrogels alone do not promote cell adhesion scaffold, fibrin serum from platelet-rich (SPRF) combined with scaffold; aim was create a intended...
The present research aimed to characterize soft tissue implants that were prepared with the use of crosslinked hyaluronic acid (HA) using two different crosslinkers and multiple reagent concentrations, alone or in combination fibrin. effect was evaluated an vivo mouse model, after 4 weeks one group 12 other. explants compared analytical methods, evaluating microscopic images, a histology analysis. kinetics degradation remodeling found be greatly dependent on concentration type crosslinker;...
There are many beneficial medical device ideas based on clinical needs and laboratory research, but development is an expensive, time-consuming complex challenge. Research quality management, which both needed to develop a device, two distinct fields, initiated by researcher or clinician having concept for it often challenging find achieve the proper steps create licensed product. Thus, in this paper, we demonstrate required mindset main of procedure through existing example, Class IIa...
Fibrin membranes are widely used in regenerative medicine because they biocompatible, biodegradable, contain growth factors, and support cell attachment. Most commonly produced from serum, but can also be isolated activated plasma. To increase the fibrinogen concentration of plasma, cryoprecipitate isolation is a possible solution. In this work, was prepared fresh frozen by plasmapheresis. The cellular elements, fibrinogen, total protein, immunoglobulins among others measured different...
Scaffolds can provide a healthy environment for cell attachment, differentiation, proliferation, and migration in vitro vivo. Lysophosphatidic acid (LPA) is naturally occurring bioactive phospholipid that present the serum mainly bound to albumin. The study aims investigate biocompatibility of LPA. It also determine effect different LPA species on proliferation human bone marrow-derived mesenchymal stem cells (hBM-dMSCs) albumin (HSA) containing scaffold development. HSA-LPA complex...