A5061825317- Periodontal Regeneration and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Bone Tissue Engineering Materials
- Mesenchymal stem cell research
- Osteoarthritis Treatment and Mechanisms
- Hemostasis and retained surgical items
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Orthopaedic implants and arthroplasty
- Blood transfusion and management
- Tendon Structure and Treatment
- Nitric Oxide and Endothelin Effects
- Proteoglycans and glycosaminoglycans research
- Reconstructive Surgery and Microvascular Techniques
- Bone and Joint Diseases
- Long-Term Effects of COVID-19
- Orthopedic Infections and Treatments
- Nanomaterials and Printing Technologies
- Skin Protection and Aging
- Blood donation and transfusion practices
- Cardiovascular Effects of Exercise
- Congenital heart defects research
- SARS-CoV-2 and COVID-19 Research
- Protease and Inhibitor Mechanisms
- Sphingolipid Metabolism and Signaling
Semmelweis University
2015-2024
Universitätsklinikum Krems
2021
Thales (Portugal)
2008
Platelet-rich fibrin (PRF) membrane is a three-dimensional biodegradable biopolymer, which consists of platelet derived growth factors enhancing cell adhesion and proliferation. It widely used in soft hard tissue regeneration, however, there are unresolved problems with its clinical application. Its preparation needs open handling the membranes, it degrades easily, has low tensile strength does not hold suture blocking wider applications PRF. Our aim was to produce sterile, suturable,...
Abstract Blood serum fractions are hotly debated adjuvants in bone replacement therapies. In the present experiment, we coated demineralized matrices (DBM) with albumin and investigated stem cell attachment vitro formation a rat calvaria defect model. experiments, observed that significantly more cells adhere to DBMs at every time point. vivo uncoated DBM was monitored biweekly by computed tomography until 11 weeks postoperatively while empty defects served as controls. By seventh week,...
Autologous blood derived products, such as platelet-rich plasma (PRP) and fibrin (PRF) are widely applied in regenerative therapies, contrast to the drawbacks their application, mainly deriving from preparation methods used. Eliminating disadvantages of both PRP PRF, hyperacute serum (HAS) opens a new path autologous therapy showing similar or even improved potential at same time. Despite frequent experimental clinical use HAS, protein composition has not been examined thoroughly yet. Thus,...
Serum albumin-coated bone allografts (BoneAlbumin) have successfully supported regeneration in various experimental models by activating endogenous progenitors. However, the effect of tissue aging, linked to declining stem cell function, has yet be explicitly examined within context BoneAlbumin's regenerative capacity. Stem function was tested with an vitro attachment assay, which showed that albumin coating increases on demineralized surfaces aging population. Bone investigated vivo...
One option to fight joint degradation and inflammation in osteoarthritis is the injection of activated blood products into synovial space. It has been demonstrated that hyperacute serum most proliferative among plasma products, so we investigated how cytokine milieu osteoarthritic knee reacts treatment vitro. Cartilage, subchondral bone, membrane explanted from knees were stimulated by interleukin-1 beta (IL-1β) concentration 39 biomarkers was measured co-culture supernatant after treatment....
Fat tissue, due to its high concentration of stem cells, has a role in aesthetic medicine and reconstructive surgery. However, poor survival the transplanted cells still limits usefulness this material regenerative medicine. Several studies indicated that platelet-rich plasma (PRP) may improve adipose tissue viability growth factor content. This study aimed at investigating effects PRP hyperacute serum (HAS) on adipogenic lineage vitro. was prepared by using two centrifugation steps presence...
Mesenchymal stem cells (MSCs) are widely used in laboratory experiments as well human cell therapy. Their culture requires animal sera like fetal calf serum (FCS) essential supplementation; however, pose a risk for clinical applications. Human blood derivatives, example, platelet-rich plasma (PRP) releasates, potential replacements of FCS; it is unclear which variant has the best effect on given or tissue type. Additionally, derivatives commonly musculoskeletal diseases osteoarthritis (OA)...
Plasma harvested from convalescent COVID-19 patients (CCP) has been applied as first-line therapy in the early phase of SARS-CoV2 pandemic through clinical studies using various protocols.We present data a cohort 267 hospitalized severe who received CCP. No transfusion-related complications were reported, indicating overall safety CCP therapy.Patients eventually died significantly later (3.95 versus 5.22 days after hospital admission) and had higher interleukin 6 (IL-6) levels (28.9 pg/ml...
S-nitrosothiol (RSNO) solutions represent a valuable source of nitric oxide and could be used as topical vasodilators, but their fast decomposition rate poses serious obstacle to potentially widespread therapeutic use. Our aim was characterize quantify the effect pH on formation in simple aqueous S-nitrosoglutathione (GSNO), S-nitroso-N-acetylcysteine (SNAC) S-nitroso-3-mercaptopropionic acid (SN3MPA). Furthermore, we investigated storage stability GSNO incorporated poly(ethylene glycol)/...
The key drawback of using demineralized bone matrix (DBM) is its low initial mechanical stability due to the severe depletion mineral content. In present study, we investigated long-term regeneration DBM in a critical size defect model and remineralization after 6 months. Bone defects were created cranium male Wistar rats which filled with or left empty as negative control. vivo formation was monitored computed tomography 11, 19, 26 weeks postoperatively. After months, parietal bones...
The use of bone allografts is contraindicated in septic revision surgery due to the high risk graft reinfection. Antibiotic release from may solve problem and these combinations can theoretically be used for prevention or even therapy infection. present study investigated whether amoxicillin, ciprofloxacin, vancomycin alone combination with chitosan alginate are suitable short-term long-term coating. Human were prepared femoral head lyophilized. coating was achieved by incubating grafts...
Hyaluronic acid (HA) is an ideal initial material for preparing hydrogels, which may be used as scaffolds in soft tissue engineering based on their advantageous physical and biological properties. In this study, two crosslinking agents, divinyl sulfone (DVS) butanediol diglycidyl ether, were to investigate effect the properties of HA hydrogels. As hydrogels alone do not promote cell adhesion scaffold, fibrin serum from platelet-rich (SPRF) combined with scaffold; aim was create a intended...
The present research aimed to characterize soft tissue implants that were prepared with the use of crosslinked hyaluronic acid (HA) using two different crosslinkers and multiple reagent concentrations, alone or in combination fibrin. effect was evaluated an vivo mouse model, after 4 weeks one group 12 other. explants compared analytical methods, evaluating microscopic images, a histology analysis. kinetics degradation remodeling found be greatly dependent on concentration type crosslinker;...
Platelet-rich plasma (PRP) and hyperacute serum (HAS) were compared in a novel human model of ex vivo bone damage induced by oxygen-glucose deprivation (OGD).Osteoarthritic subchondral pieces harvested from discarded femoral heads during hip replacement surgery subjected to transient OGD.Proteome profiling revealed that PRP is more angiopoietic, whereas HAS antiangiopoietic composition. However, treatment OGD-exposed with multiple preparations had no effect on cell counts, restored...
There are many beneficial medical device ideas based on clinical needs and laboratory research, but development is an expensive, time-consuming complex challenge. Research quality management, which both needed to develop a device, two distinct fields, initiated by researcher or clinician having concept for it often challenging find achieve the proper steps create licensed product. Thus, in this paper, we demonstrate required mindset main of procedure through existing example, Class IIa...
The occurrence of physical and psychological symptoms that negatively influence everyday activities, efficient job performance, professional patient treatment are common among paramedic workers. Aim: We uncovered the characteristic coping strategies applied by on-site paramedics searched for correlations between these demographic, socio-economic characteristics, as well factors related to work, workplace, health behaviours. Sample methodology: survey was conducted March 1, 2018, February 28,...
In this present study, the material science background of crosslinked gelatin (GEL) was investigated. The aim to assess optimal reaction parameters for production a water-insoluble matrix suitable heat sterilization. Matrices were subjected enzymatic degradation assessments, and their ability withstand sterilization evaluated. impact different crosslinkers on properties analyzed. It found that matrices with butanediol diglycidyl ether (BDDE) poly(ethylene glycol) (PEGDE) resistant...