Both hyperandrogenism (HA) and vitamin D deficiency (VDD) can separately lead to impaired vascular reactivity ovulatory dysfunction in fertile females. The aim was examine the early interactions of these states a rat model PCOS. Four-week-old adolescent female rats were divided into four groups: (VD)-supplemented (n = 12); VD-supplemented testosterone-treated VDD- 11) VDD-and-testosterone-treated 11). Animals underwent transdermal testosterone treatment for 8 weeks. Target VD levels achieved...

Carotid artery stenosis (CAS) affects approximately 5–7.5% of older adults and is recognized as a significant risk factor for vascular cognitive impairment (VCI). The impact CAS on cerebral blood flow (CBF) within the ipsilateral hemisphere relies adaptive capabilities microcirculation. In this study, we aimed to test hypothesis that impaired availability nitric oxide (NO) compromises CBF homeostasis after unilateral carotid occlusion (CAO). To investigate this, three mouse models exhibiting...

Hyperandrogenic state in females is accompanied with metabolic syndrome, insulin resistance and vascular pathologies. A total of 67%-85% hyperandrogenic women suffer also from vitamin D deficiency. We aimed to check a potential interplay between hyperandrogenism deficiency producing effects on coronary arteries. Adolescent female rats were divided into four groups, 11-12 animals each. Transdermal testosterone-treated vehicle-treated kept either D-deficient or D-supplemented diet for 8 weeks....

Vitamin D deficiency (VDD) is a global health problem, which can lead to several pathophysiological consequences including cardiovascular diseases. Its impact on the cerebrovascular system not well understood. The goal of present work was examine effects VDD morphological, biomechanical and functional properties cerebral arterioles.Four-week-old male Wistar rats (n = 11 per group) were either fed with vitamin deficient diet or received conventional rat chow os supplementation. Cardiovascular...

Hyperandrogenism is a risk factor of cerebrovascular diseases as androgens can alter markedly the regulation tone. We examined combined impact androgen excess and vitamin D deficiency (VDD), common co-morbidity in hyperandrogenic disorders, on remodeling testosterone-induced vascular responses anterior cerebral arteries (ACA) order to evaluate interplay between VDD vasculature. Male female Wistar rats were either fed with deficient or supplemented diet. Half animals from both groups received...

Hyaluronic acid (HA) is an ideal initial material for preparing hydrogels, which may be used as scaffolds in soft tissue engineering based on their advantageous physical and biological properties. In this study, two crosslinking agents, divinyl sulfone (DVS) butanediol diglycidyl ether, were to investigate effect the properties of HA hydrogels. As hydrogels alone do not promote cell adhesion scaffold, fibrin serum from platelet-rich (SPRF) combined with scaffold; aim was create a intended...

Vitamin D insufficiency has been associated with increased incidence and severity of cerebrovascular disorders. We analyzed the impact impaired vitamin signaling on anatomical functional aspects adaptation to unilateral carotid artery occlusion (CAO), a common consequence atherosclerosis cause ischemic stroke. Cerebrocortical blood flow (CoBF) showed significantly drop delayed recovery after CAO in mice carrying functionally inactive receptor (VDR) most sustained perfusion deficit temporal...

Several reports prove interconnection between vitamin D (VD) deficiency and increased cardiovascular risk. Our aim was to investigate the effects of VD status on biomechanical oxidative-nitrative (O-N) stress parameters coronary arterioles in rats.4-week-old male Wistar rats were divided into a control group (11 animals) with optimal supply (300 IU/kgbw/day) VD-deficient animals, <5 IU/kg/day). After 8 weeks, arteriole segments prepared. Geometrical, elastic, characteristics measured by...

Abstract Background Biomechanical remodeling of coronary resistance arteries in physiological left ventricular hypertrophy has not yet been analyzed, and the possible sex differences are unknown. Methods Wistar rats were divided into four groups: male female sedentary controls (MSe FSe) animals undergoing a 12-week intensive swim training program (MEx FEx). On last day, vitro contractility, endothelium-dependent dilatation, biomechanical properties intramural investigated by pressure...

The present research aimed to characterize soft tissue implants that were prepared with the use of crosslinked hyaluronic acid (HA) using two different crosslinkers and multiple reagent concentrations, alone or in combination fibrin. effect was evaluated an vivo mouse model, after 4 weeks one group 12 other. explants compared analytical methods, evaluating microscopic images, a histology analysis. kinetics degradation remodeling found be greatly dependent on concentration type crosslinker;...

Clinical practice gives medical students opportunities to develop clinical skills and gain insight into their future profession as a physician. Students in the programme at Karolinska Institutet Sweden had primary health care nine of 11 semesters. The aim this study was explore students' perceptions learning from patient encounters context.The qualitative inductive interview study. 21 participating were 3rd, 4th 5th (final year) year programme. A semi-structured guide used. data analysis...

Vitamin D (vitD) insufficiency affects 1 billion people worldwide. Androgen excess (AE) occurs in 8% of fertile females. There are few data about the combined effect vitD deficiency and AE on early biomechanical changes cerebral arterioles fertile-aged female. Forty-six adolescent female Wistar rats (21-28 day-old, weighing 90-110 g) were grouped randomly four groups: supplemented groups with without transdermal testosterone (T) treatment, as well deficient also T (n = 11 or 12, all cases)....

Vitamin D deficiency contributes to the pathogenesis of age-related cerebrovascular diseases, including ischemic stroke. Sex hormonal status may also influence prevalence these disorders, indicated by a heightened vulnerability among postmenopausal and hyperandrogenic women. To investigate potential interaction between sex steroids disrupted vitamin signaling in cerebral microcirculation, we examined adaptation unilateral carotid artery occlusion (CAO) intact, ovariectomized, female mice...

BACKGROUND: Exercise training is associated with hypertrophy of left ventricle (LV). The aim the present study to evaluate sex differences in adaptation coronary contractile function physiological ventricular induced by long-term swim training.METHODS: Thirty-two Wistar rats were randomly divided into 4 groups: exercised male (ExM), female (ExF), untrained control (CoM), and (CoF). trained animals underwent a 12-week-long program. After finishing program, LV morphology checked...

The understanding of cerebral autoregulation has become notable importance due to the increased incidence carotid artery stenosis in elderly population which entails an risk stroke, 2nd most common cause mortality worldwide. Many debates, regarding its exact mechanisms, exist scientific literature including role endothelial and neuronal nitric oxide (NO) synthases (eNOS nNOS) prostanoid mediators (PMs). According our previous observations, eNOS, surprisingly, does not seem play important...

Objective: Polycystic ovarian syndrome (PCOS) one of the most frequent gynecological illnesses fluencing about 8% fertile women worldwide. Beyond hyperandrogenic state, vitamin D hypovitaminosis occurs in 70–80% suffering from PCOS. Our aim was to investigate combined effect these hormonal alterations, considering insulin and testosterone induced relaxation coronary arterioles. Design method: 46 adolescent (21–28 day-old), 90–110 gram-weighed female Wistar rats were grouped randomly 4...

Objective: Vitamin D deficiency (VDD) is a global health problem, which can lead to several pathophysiological consequences including cardiovascular diseases. Its impact on the cerebrovascular system not well understood. The goal of present work was examine effects VDD morphological, biomechanical and functional properties cerebral arterioles. Design method: Four-week-old male Wistar rats (n = 11 per group) were either fed with vitamin deficient diet or received conventional rat chow os...

Objective: ‘Athlets heart’, which means hypertrophy and remodeling of left ventricular tissue induced by heavy exercises, is known searched for a long time. The increased muscle mass would need an perfusion require the coronary circulation. But arterioles adaptation in this process not well influence gender on at all, so far. We aimed to study differences sport-adaptation coronaries. Design method: Young adult Wistar rats were distributed into four groups, Male Female sedentary animals...

Objective: PCOS and vitamin D deficiency are both frequent multifactorial endocrine disorders, lead to metabolic early cardiovascular complications. According actual data, there an estimated 10.5 million fertile women suffering from only in the US. With this study we would like describe biomechanical changes of small coronary arterioles leading a higher risk cardial cerebral insult. Design method: 46 adolescent (21–28 day-old), 90–110 gramm-weighed female Wistar rats were grouped randomly 4...

Abstract Objectives The cardiovascular/CV risk is higher in males. To know better these increased risks, our aim was to investigate the effects of different vitamin D/VD status on mechanical, pharmacological and histological characteristics coronary arterioles male rat model. Methods In Wistar-rats we induced VD status: “Group C” got 1000 IU containing chow VD-supplementation, D-” less, than 5 model deficient/VDD status. At end experiment prepared intramural, small arteriole segments from...