A5040021775- Viral-associated cancers and disorders
- Cytomegalovirus and herpesvirus research
- Parvovirus B19 Infection Studies
- Plant Micronutrient Interactions and Effects
- Plant Stress Responses and Tolerance
- Lymphoma Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Photosynthetic Processes and Mechanisms
- Histiocytic Disorders and Treatments
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- Virus-based gene therapy research
- Nuclear Structure and Function
- Circular RNAs in diseases
- RNA modifications and cancer
- Identification and Quantification in Food
- Herpesvirus Infections and Treatments
- Epigenetics and DNA Methylation
- Chronic Lymphocytic Leukemia Research
- Cardiac Fibrosis and Remodeling
- Salmonella and Campylobacter epidemiology
- Polyomavirus and related diseases
- T-cell and Retrovirus Studies
- Milk Quality and Mastitis in Dairy Cows
- Genetics and Neurodevelopmental Disorders
Structural Analytics (United States)
2020-2022
Montavid Thermodynamic Research Group
2013-2021
National Center for Epidemiology
2007-2013
Autologous blood derived products, such as platelet-rich plasma (PRP) and fibrin (PRF) are widely applied in regenerative therapies, contrast to the drawbacks their application, mainly deriving from preparation methods used. Eliminating disadvantages of both PRP PRF, hyperacute serum (HAS) opens a new path autologous therapy showing similar or even improved potential at same time. Despite frequent experimental clinical use HAS, protein composition has not been examined thoroughly yet. Thus,...
Epstein-Barr virus (EBV) is an important human pathogenic gammaherpesvirus with carcinogenic potential. The EBV transcriptome has previously been analyzed using both Illumina-based short read-sequencing and Pacific Biosciences RS II-based long-read sequencing technologies. Since the various methods have distinct strengths limitations, use of multiplatform approaches proven to be valuable. aim this study provide a more complete picture on transcriptomic architecture EBV.In work, we apply...
Here, we wish to highlight the genetic exchange and epigenetic interactions between Epstein-Barr virus (EBV) its host. EBV is associated with diverse lymphoid epithelial malignancies. Their molecular pathogenesis accompanied by alterations which are distinct for each of them. While lymphoblastoid cell lines derived from B cells transformed in vitro characterized a massive demethylation euchromatinization viral cellular genomes, primarily malignant tumor Burkitt's lymphoma tumors...
One option to fight joint degradation and inflammation in osteoarthritis is the injection of activated blood products into synovial space. It has been demonstrated that hyperacute serum most proliferative among plasma products, so we investigated how cytokine milieu osteoarthritic knee reacts treatment vitro. Cartilage, subchondral bone, membrane explanted from knees were stimulated by interleukin-1 beta (IL-1β) concentration 39 biomarkers was measured co-culture supernatant after treatment....
A large percentage of primary sensory neurons in the trigeminal ganglia (TG) contain neuropeptides such as tachykinins or calcitonin gene-related peptide. Neuropeptides released from central terminals afferents sensitize secondary nociceptive nucleus caudalis (TNC), but also activate glial cells contributing to neuroinflammation and consequent sensitization chronic orofacial pain migraine. In present study, we investigated newest member tachykinin family, hemokinin-1 (HK-1) encoded by Tac4...
ABSTRACT We analyzed the levels of acetylated histones and histone H3 dimethylated on lysine 4 (H3K4me2) at LMP2A promoter (LMP2Ap) Epstein-Barr virus in well-characterized type I III lymphoid cell line pairs additionally nasopharyngeal carcinoma C666-1 by using chromatin immunoprecipitation. found that enhanced marked upregulated LMP2Ap cells. In contrast, cells, highly DNA-methylated, inactive was also enriched H3K4me2. Our results suggest combinatorial effects DNA methylation,...
In this study, the binding of insulator protein CCCTC-binding factor (CTCF) to region located between Rep* and C promoter (Cp) Epstein–Barr virus (EBV) was analysed using chromatin immunoprecipitation in vivo footprinting. CTCF found be independent Cp usage cell lines corresponding major EBV latency types. Bisulfite sequencing an electrophoretic mobility-shift assay (using methylated unmethylated probes) revealed that insufficient induce local CpG demethylation certain unaffected by...
The EBV carrying lines MEC1 and MEC2 were established earlier from explants of blood derived cells a chronic lymphocytic leukemia (CLL) patient at different stages progression to prolymphocytoid transformation (PLL). This pair is unique in several respects. Their common clonal origin was proven by the rearrangement immunoglobulin genes. driven proliferation vitro same indigenous strain. They are phenotypically represent subsequent subclones emerging CLL population. Furthermore they reflect...
Chronic lymphocytic leukemia (CLL) is characterized by a neoplastic B-cell population coexpressing CD5 and CD23; however, the expression of CD23 variable. In human, two isotypes have been identified related to different functions. The aim our study was investigate relative in CLL find possible correlation with other prognostic factors. analyzed 54 cases polymerase chain reaction (PCR) quantitative real-time PCR. immunophenotype cells flow cytometry. We demonstrated higher CD23a than CD23b...
The accumulation of NiCo following the termination iron in chloroplast suggests that is not solely involved uptake processes chloroplasts. Chloroplast (Fe) thought to be operated by a complex containing permease 1 (PIC1) and nickel-cobalt transporter (NiCo) proteins, whereas role other Fe homeostasis-related transporters such as multiple antibiotic resistance protein (MAR1) less characterized. Although pieces information exist on regulation uptake, including effect plant homeostasis, whole...
Objective: We report the infection of New Zealand white rabbits with Epstein-Barr virus (EBV). Methods: EBV prepared in B95-8 (producer) cells was inoculated to by combined intranasal and oral routes. Blood blood cell (WBC) samples were taken before infection, then on days 8, 28 98 post-infection (p.i.). Results: Administration either 3 × 108 (group A, 11 rabbits) or 1 109 B, 10 DNA copies per animal induced subacute and/or persistent infection. The IgG antibodies plasma detected ELISA as...
The objective of this study was to find an efficient, rapid, simple, and cost-effective method pretreating raw milk samples produce PCR-ready DNA for subsequent microbial detection using the strains eight bacterial species. A total 17 in-house protocols three commercial kits were evaluated in steps from scientific, practical, economic perspectives. results showed that procedure involving Triton X-100-based pretreatment inhibitor removal resin superior all other methods tested terms yield,...
Iron (Fe) is an essential micronutrient for plants. Due to the requirement Fe of photosynthetic apparatus, majority shoot content localised in chloroplasts mesophyll cells. The reduction-based mechanism has prime importance uptake operated by Ferric Reductase Oxidase 7 (FRO7) inner chloroplast envelope membrane. Orthologue Arabidopsis thaliana FRO7 was identified Brassica napus genome. GFP-tagged construct BnFRO7 showed integration chloroplast. time-scale expression pattern studied under...
Microorganisms, regardless of whether pathogenic or not, may cause enormous economic losses due to adverse effects on human and animal health, by damaging the quality agricultural food products. Based these effects, development prompt molecular methods their involvement in practical pathogen diagnostic diagnostics is more than actual. This paper focused evaluation easy-to-perform highly budget-friendly, PCR-related DNA purification protocols for purposes especially water similar simple...
MicroRNAs (miRNAs) are posttranscriptional regulators fine-tuning the level of most messenger RNAs (mRNAs) and proteins in mammalian cells. Their expression is dysregulated neoplastic cells upregulated or downregulated miRNAs play an important role tumorigenesis. Changes miRNA transcriptome appear to be suitable markers for differential diagnosis various B-cell lymphoma types there therapeutic attempts normalize key cellular involved lymphomagenesis. In this review we wish outline recent...
Lamin A, B and C, the nuclear intermediate-filament proteins, play a role in epigenetic regulation.Lamin could be detected all nucleated cells studied, whereas lamin A C isoforms (lamin A/C) encoded by LMNA gene are co-expressed most somatic cell types except mature lymphocytes.Since Epstein-Barr virus (EBV), human gammaherpesvirus, is associated with tumorigenic processes known to alter epigenotype of its host cells, we studied expression marks EBV-carrying lymphoid lines.We observed high...
Astaxanthin is a lipid-soluble carotenoid influencing lipid metabolism, body weight, and insulin sensitivity. We provide systematic analysis of acute chronic effects astaxanthin on different organs. Changes by feeding were analyzed general expression regulatory proteins in the skeletal muscle, as well changes excitation synaptic activity hypothalamic arcuate nucleus mice. Acute responses also tested canine cardiac muscle neuronal populations Dietary significantly increased food intake. It...