Bacterial pneumonia is a common disease in dairy herds worldwide, which brings great economic losses to farmers. Sodium butyrate (SB), an inhibitor of histone deacetylase, plays important role limiting inflammation. The purpose this study was investigate the protective effects SB on lipopolysaccharide (LPS)-induced acute lung injury (ALI) mice and explore potential mechanism protection. A total 30 ICR were randomly divided into three groups (n = 10): phosphate-buffered saline (PBS)...

Although high-concentrate diet feeding can temporarily increase milk production, it cause a series of metabolic diseases, such as subacute ruminal acidosis (SARA) and fat depression. The main purpose this experiment was to study the effects on inflammatory response, oxidative stress, synthesis in mammary gland dairy cows. Twelve Holstein cows equipped with rumen fistulas were randomly divided into 2 groups, each 6 cows, fed low-concentrate (LC) (HC). On d 20 21 experiment, fluid collected...

Ruminal dysbiosis-induced liver injury is prevalent in dairy cows, yet its underlying mechanisms remain incompletely understood. Ferroptosis, a newly identified form of programmed cell death distinct from apoptosis and necrosis, has been implicated various diseases by emerging studies. In the present study, lipopolysaccharide (LPS) γ-D-glutamyl-meso-diaminopimelic acid (iE-DAP) were employed to establish vitro vivo models using bovine hepatocytes mice, respectively. It was observed that...

Two experiments were conducted to study the effect of supplemental 100 g/day live Bacillus cultures (2 × 1011 cell subtilis and licheniformis) on rumen fermentation as well milk yield composition in Chinese Holstein cows. In experiment 1, investigating 3 10 cows, protein increased by using B. licheniformis (p < 0.05) comparison with an unsupplemented group group. Body weight was not significantly affected culture supplementation > 0.05). Percentage fat lactose different between treatments...

Long-term feeding of a high-concentrate diet can induce subacute ruminal acidosis (SARA) in ruminants, which further leads to systemic inflammatory response. However, few studies have examined the effects on hindgut ruminants. The purpose this study was investigate composition gut microbiota colonic contents, response, and barrier damage colon tissue A total 12 healthy multiparous lactating Hu sheep were randomly allotted into following 2 groups: (HC) group (concentrate:forage = 7:3)...

The purpose of this experiment was to investigate high-concentrate feeding-induced changed status oxidative and autophagy in the livers dairy cows. Hepatocyte nuclear factor 3β (FOXA2) reported cases liver fibrosis, glucolipid metabolism, hepatocyte differentiation, but not damage cows fed a diet. Therefore, we also aimed explore potential role FOXA2 SARA-induced damage. We divided 12 mid-lactating Holstein into 2 groups them (HC group, forage:concentrate = 4:6) low-concentrate...

Asherman syndrome (intrauterine adhesion) is often associated with menstrual abnormalities, infertility and recurrent miscarriage in female. Currently the molecular mechanism regulating pathogenesis of not known. Here we revealed that inflammatory factor NF-κB expression significantly elevated endometrial samples patients. To further study mechanisms, established an rat model confirmed important role syndrome. In addition, our provided direct evidence intrauterine adhesion results impaired...

<h2>ABSTRACT</h2> Mitochondrial homeostasis is closely associated with cellular process, whereas mitochondrial dysfunction contributes to apoptosis and mitophagy. Hence, analyzing the mechanism of lipopolysaccharide (LPS)-caused damage necessary understand how maintained in bovine hepatocytes. Mitochondria-associated membranes (MAM), a connection between endoplasmic reticulum (ER) mitochondria, important control function. To investigate underlying mechanisms LPS-caused dysfunction,...

Oxidative stress is tightly associated with liver dysfunction and injury in dairy cows. Previous studies have shown that cis-9, trans-11 conjugated linoleic acid (CLA) possesses anti-inflammatory antioxidative abilities. In this study, the bovine hepatocytes were pretreated CLA for 6 h, followed by treatment hydrogen peroxide (H2O2) another h to investigate effect of uncover underlying mechanisms. The results demonstrated H2O2 elevated level mitophagy, promoted mitochondrial DNA (mtDNA)...

Subacute ruminal acidosis can cause liver injury in ruminants. Ferroptosis, an iron-dependent cell death, is involved many diseases. This study aimed to investigate ferroptosis SARA-induced and explore the changes hepatic iron metabolism. Twelve ruminally cannulated, lactating Hu sheep (parities: 2 or 3; BW: 50.6 ± 4.0 kg; 18.8 3.6 d milk; MY: 0.52 0.08 kg/d; mean SD) were divided into groups (n = 6/group) fed a low-grain diet (LG group, grain: forage 3: 7, 24.89% starch 40.66% NDF)...

Currently, little is known about the effect of sodium butyrate (NaB) on oxidative stress following grain-induced sub-acute ruminal acidosis in dairy goats. In present study, 18 lactating goats implanted with a cannula and permanent indwelling catheters portal hepatic veins were randomly allocated into 3 treatment groups over 20 weeks: low grain (LG, 40% grain; n = 6), high (HG, 60% 6) (HG + NaB, NaB; 6). When added to HG diet, NaB increased mean pH reduced levels ruminal, LPS; Additionally,...

The aim of the present study was to evaluate how long-term high-concentrate diet feeding affected rumen epithelium (RE) dairy cows. So, 12 mid-lactating multiparous cows were divided into two groups randomly fed either with (HC, concentrate: forage = 6: 4) or low-concentrate (LC, 4:6) for 20 weeks. Remarkable upregulation lipopolysaccharide (LPS) level and depress pH in fluid induced by HC compared LC group. mRNA abundance interleukin-6 (IL-6), interleukin-8 (IL-8), C-C motif chemokine...

Lipopolysaccharide (LPS) is the dominating endotoxin of Gram-negative bacteria, which can cause mastitis. Bovine mammary epithelial cells (BMECs), as major components gland, usually suffer LPS challenge. Cis-9, trans-11 conjugated linoleic acid (CLA) has been reported to have anti-inflammatory characteristics, while its anti-oxidative ability maintain cellular homeostasis in BMECs under challenge limited. Therefore, we studied whether cis-9, CLA restore disturbance indicated by redox status...

High-concentrate feeding can induce subacute ruminal acidosis, which leads to mammary tissue injury in dairy cows. Therefore, the purpose of this research was evaluate effect high-concentrate on STIM1 (stromal interaction molecule 1)/ORAI1 (Orai calcium release-activated modulator 1)-mediated inflammation, endoplasmic reticulum stress (ERS), and apoptosis A total 12 healthy mid-lactating Holstein cows similar weight were randomly allotted into following 2 groups: a (HC) group...

Ketosis is a metabolic disease that often occurs in dairy cows postpartum and results from disordered lipid metabolism. Acetyl-coenzyme A (CoA) acetyltransferase 2 (ACAT2) important for balancing cholesterol triglyceride (TG) metabolism; however, its role subclinical ketotic unclear. This study aimed to explore the potential correlation between ACAT2 metabolism disorders through vitro vivo experiments. In experiment, liver tissue blood samples were collected healthy (CON, n = 6,...

High-grain (HG) feeding can trigger subacute ruminal acidosis (SARA) and subsequent liver tissue injury. This study investigated pyroptosis NLRP3 inflammasome activation in SARA-induced injury, the role of mitophagy during this process. Twelve mid-lactating Holstein cows equipped with rumen fistulas were randomly divided into 2 groups: a low-grain (LG) diet group (grain:forage = 4:6) HG 6:4). Each had 6 cows. The experiment lasted for 3 wk. fluid was collected through fistula on experimental...

This study aimed to evaluate whether sodium butyrate (SB) attenuates the hepatic response LPS-induced inflammation in bovine hepatocytes. Hepatocytes isolated from cows at ∼160 days milk (DIM) were exposed 0.5 mmol/L SB for 18 h as pretreatment. Cells pretreated with used group, and those subjected 4 μg/mL lipopolysaccharide (LPS) challenge 6 (LSB) group. The LPS-challenged hepatocytes showed increases TNF-α IL-6 production culture medium (37 ± 11, P < 0.05); these attenuated by pretreatment...

The present study aims to reveal the mechanisms of hepatocyte apoptosis induced by dietary feeding. Eighteen midlactating goats were randomly divided into three groups: low concentrate group (LC), high (HC), and sodium butyrate (SB)-supplemented (SHC). After 10 weeks, HC diet successfully subacute ruminal acidosis (SARA), which increased lipopolysaccharide (LPS) cytokine concentrations expression genes proteins related inflammation apoptosis. addition SB notably decreased levels those...