A5085426043- Cervical Cancer and HPV Research
- Genital Health and Disease
- Colorectal and Anal Carcinomas
- Molecular Biology Techniques and Applications
- Reproductive tract infections research
- Cancer and Skin Lesions
- Hepatitis B Virus Studies
- Tumors and Oncological Cases
- Urological Disorders and Treatments
- Genetic factors in colorectal cancer
- Global Public Health Policies and Epidemiology
- Urinary and Genital Oncology Studies
- HIV/AIDS oral health manifestations
- Healthcare Policy and Management
- Genetic and rare skin diseases.
- Endometrial and Cervical Cancer Treatments
- Viral-associated cancers and disorders
- Ovarian cancer diagnosis and treatment
- Cancer-related molecular mechanisms research
- Health disparities and outcomes
- Animal Virus Infections Studies
- Epigenetics and DNA Methylation
- Nonmelanoma Skin Cancer Studies
- Colorectal Cancer Screening and Detection
- Cancer-related gene regulation
Leiden University Medical Center
2021-2022
DDL Diagnostic Laboratory
2016-2020
Cervical cancer incidence and mortality rates in Sub-Saharan Africa (SSA) remain high due to several factors including low levels of uptake cervical screening. Self-collection cervicovaginal samples for HPV DNA testing may be an effective modality that can increase screening SSA hard reach populations developed countries. We investigated whether self-collection tests would associated with increased compared clinic based collection samples. Furthermore, we the quality collected by both...
To compare the sensitivity of high-risk human papillomavirus (hrHPV) and genotype detection in self-collected urine samples morning (U1), later on (U2), brush-based self-samples (SS), clinician-taken smears (CTS) for detecting cervical intraepithelial neoplasia grade 2+ (CIN2+) a colposcopic referral population.Cross-sectional single-centre study.A colposcopy clinic Spain.A cohort 113 women referred after an abnormal Pap smear.Women undergoing with biopsy were sent device (Colli-Pee™,...
In this study, we evaluate the expression of human papillomavirus E4 protein (marker for onset a productive infection) and hypermethylation host-cell CADM1, MAL, miR124-2 genes an advanced, transforming in cervical intraepithelial neoplasia (CIN) cancer. A total 115 lesions were categorized by 3 pathologists into no dysplasia, CIN1, CIN2, CIN3, or cancer classical histomorphological grading criteria, immunoscore (cumulative value: 0-6) system based on Ki-67 (score: 0-3) p16ink4a expression....
Aims To investigate the accuracy and reproducibility of a scoring system for cervical intraepithelial neoplasia (CIN1–3) based on immunohistochemical (IHC) biomarkers Ki-67 p16 ink4a . Methods 115 tissue specimens were reviewed by three expert gynaecopathologists graded according to strategies: (1) CIN grade H&E staining only; (2) immunoscore cumulative score only (0–6); (3) supported non-objectified IHC 2 weeks after 1 2. The majority consensus diagnosis was used as Reference Standard...
Cervical screening aims to identify women with high-grade squamous intraepithelial lesion/cervical neoplasia 2-3 (HSIL/CIN2-3) or invasive cervical cancer (ICC). Identification of severe premalignant lesions ICC (CIN3+) could ensure their rapid treatment and prevent overtreatment. We investigated high-risk human papillomavirus (hrHPV) detection genotyping methylation FAM19A4/miR124-2 for CIN3+ in 538 attending colposcopy abnormal cytology. All had an additional cytology hrHPV testing...
Abstract The decision to treat a cervical squamous intraepithelial lesion (SIL) by loop electrosurgical excision procedure (LEEP) relies heavily on colposcopy‐directed biopsy showing high‐grade (H)SIL. Diagnosis is often supported p16, an immunohistochemical (IHC) biomarker of high‐risk (hr)HPV E7 gene activity. Additional potential markers include methylation tumor suppressor genes FAM19A4/miR124‐2 in cytology for advanced transforming HSIL and the IHC marker HPV E4 productive, potentially...
Because current guidelines recognise high-grade anal squamous intraepithelial lesions (HSILs) and low-grade SILs (LSILs), recommend treatment of all HSILs although not progress to cancer, this study aims distinguish transforming productive by grading immunohistochemical (IHC) biomarkers p16
Our objective was to identify virological and serological predictors of anal high-grade squamous intraepithelial lesions (HSIL) in human immunodeficiency virus (HIV)-positive men who have sex with (MSM).HIV-positive MSM were recruited from a longitudinal study during which self-swabs serum collected at up 5 bi-annual visits. Swabs papillomavirus (HPV) genotyped, the type-specific HPV viral load swabs determined. Serum antibodies E6, E7, E1, E2, L1 proteins 7 high-risk (hrHPV) types HPV6 11...
Progression of anal intraepithelial neoplasia (AIN) involves transition from productive to transforming human papillomavirus (HPV) infection. Grading aims distinguish low-grade AIN high-grade (HGAIN) with risk cancer. We describe immunohistochemical patterns in adding a novel marker for initiation the phase HPV life cycle (panHPVE4) those cell activity (Ki-67) and HPVE7 gene (p16). studied 67 biopsies suspected (17 normal, 15 AIN1, 20 AIN2, AIN3) 54 men who have sex at New York Presbyterian...
The aim of this study is to determine whether immunohistochemistry for Stathmin-1 enhances diagnostic accuracy anal dysplasia. included 40 biopsies with diagnosis benign transitional zone (n=10), low-grade intraepithelial neoplasia (AIN) (AIN1, n=10), and high-grade AIN (AIN2, n=10, AIN3, n=10). cases were selected represent classic features. Immunohistochemistry Stathmin-1, p16, Ki-67 was performed assessed distribution within epithelial thickness. expressed only in the basal layer...
Our objective was to identify virological and serological predictors of anal high-grade squamous intraepithelial lesions (HSIL) in HIV-positive men-who-have-sex-with-men (MSM). MSM were recruited from a longitudinal study (2010–2013), during which self-swabs serum collected at up five bi-annual visits. Swabs HPV genotyped, type-specific viral load swabs determined. Serum antibodies E6, E7, E1, E2 L1 proteins 7 hrHPV-types HPV6 11 analyzed. 193 participants had high-resolution anoscopy (HRA)...
Trichoblastomas are rare dermal neoplasms usually found on the scalp and face. Histology shows a proliferation of small basaloid cells arranged in cords or fields surrounded by cellular stroma. not aggressive, but trichoblastic carcinomas arising from preexisting trichoblastomas have been described linked to basal cell carcinoma. We tumor with features trichoblastoma presence Merkel cells, deeply infiltrative growth pattern into fat muscle tissue, without significant architectural atypia....
Objectives: To compare the SPF10 system to GP5+/6+ for high risk HPV (hrHPV) screening on cervicovaginal samples in Nigeria. Methods: In Karu, a suburban district of Abuja, Nigeria, 400 women were randomized self- or hospital-collection after flyer recruitment. A volume 250 μL was used DNA isolation with MagNA Pure and 750 nucliSENS easyMAG isolation. isolates tested SPF10-PCR-DEIA-LiPA25, version 1 system, test often epidemiological studies. GP5+/6+-PCR-EIA, clinically validated cervical...
Objectives: To assess the feasibility of Precursor-M methylation test as a triage marker on self-collected and hospital-collected samples amongst hrHPV+ population in resource limited setting. Methods: A total 400 women were invited to participate through flyer recruitment Abuja, Nigeria. After randomization, 200 asked self-collect deposit cervical at designated collection points hospital for sample (HCS). dry flocked swab was used both groups. DNA isolated with nucliSENS easyMAG, tested...
Immunohistochemical (IHC) biomarkers enable standardised, reproducible grading of anal intraepithelial neoplasia (AIN) based on progression HPV infection from transient productive to complete neoplastic transformation. We investigated the expression IHC biomarker E4, a marker infection, in different grades AIN caused by low-risk (lr) and high-risk (hr) infection. A reference was established using expert, consensus, subjective HE diagnoses supported p16 for 183 biopsies: normal squamous...