A5000150825- Reproductive System and Pregnancy
- Congenital Diaphragmatic Hernia Studies
- Neonatal Respiratory Health Research
- Endometriosis Research and Treatment
- Congenital Anomalies and Fetal Surgery
- Pregnancy and preeclampsia studies
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Cancer-related molecular mechanisms research
- Endometrial and Cervical Cancer Treatments
- Ovarian cancer diagnosis and treatment
- RNA Research and Splicing
- Immune cells in cancer
- Reproductive Physiology in Livestock
- Circular RNAs in diseases
- Eosinophilic Esophagitis
- RNA modifications and cancer
- Long-Term Effects of COVID-19
- Reproductive Biology and Fertility
- Immune Cell Function and Interaction
- Cancer-related gene regulation
- T-cell and Retrovirus Studies
- Gynecological conditions and treatments
- Animal Nutrition and Physiology
- Molecular Biology Techniques and Applications
Hospital for Sick Children
2020-2025
Biologie du Développement et Cellules Souches
2022-2025
SickKids Foundation
2024
Queen's University
2014-2022
Division of Undergraduate Education
2022
Lunenfeld-Tanenbaum Research Institute
2019
Marine Biological Laboratory
2017
University of Chicago
2017
Fundação Oswaldo Cruz
2016
University of Guelph
2013-2015
Abstract Exosomes and microvesicles are extracellular vesicles released from cells can contain lipids, miRNAs proteins that affect at distant sites. Recently, containing miRNA have been implicated in uterine microenvironment of pigs, a species with unique epitheliochorial (non-invasive) placentation. Here we report novel role conceptus-derived exosomes/microvesicles (hereafter referred to as vesicles; EVs) embryo-endometrial cross-talk. We also demonstrate the stimulatory effects EVs...
High-grade serous carcinoma (HGSC) of the ovary is predominantly diagnosed at late stages and primarily treated with debulking surgery followed by platinum/taxane-based chemotherapy. Although certain patients benefit significantly from currently used chemotherapy, there are who either do not respond or have an inadequate duration response. We previously showed that tumours chemoresistant immunosuppressed pre-existing tumour immune microenvironment decreased expression Type I Interferon...
With multifactorial etiologies, combined with disease heterogeneity and a lack of suitable diagnostic markers therapy, endometriosis remains major reproductive health challenge. Extracellular vesicles (EVs) have emerged as contributors progression in several conditions, including variety cancers; however, their role pathophysiology has remained elusive. Using next-generation sequencing EVs obtained from patient tissues plasma samples compared controls, we documented that carry unique...
Abstract Endometriosis is a debilitating condition that categorized by the abnormal growth of endometrial tissue outside uterus. Although pathogenesis this disease remains unknown, it well established endometriosis patients exhibit immune dysfunction. Interleukin (IL)-33 danger signal critical regulator chronic inflammation. plasma and peritoneal fluid levels IL-33 have been associated with deep infiltrating endometriosis, its contribution to pathophysiology unknown. We investigated role in...
Endometriosis is an estrogen dependent, chronic inflammatory disease characterized by the growth of endometrial lining outside uterus. Mast cells have emerged as key players in regulating not only allergic responses but also other mechanisms such angiogenesis, fibrosis, and pain. The influence on mast cell function has been recognized a potential factor driving pathophysiology number conditions. However, precise information lacking cross talk between endocrine immune factors within...
Placental growth factor (PGF) is expressed in the developing mouse brain and contributes to vascularization vessel patterning.PGF dynamically fetal brain, particularly forebrain, essential for normal cerebrovascular development.PGF rises maternal plasma over human pregnancy but low many women with acute onset hypertensive syndrome, pre-eclampsia (PE). Little known about expression of PGF fetus during PE. Pgf (-/-) mice appear recently cerebral vascular defects were documented adult...
Antenatal administration of extracellular vesicles from amniotic fluid stem cells (AFSC-EVs) reverses features pulmonary hypoplasia in models congenital diaphragmatic hernia (CDH). However, it remains unknown which lung cellular compartments and biological pathways are affected by AFSC-EV therapy. Herein, we conducted single-nucleus RNA sequencing (snRNA-seq) on rat fetal CDH lungs treated with vehicle or AFSC-EVs. We identified that intra-amniotically injected AFSC-EVs reach the rats CDH,...
MicroRNAs (miRNAs) post-transcriptionally regulate a vast network of genes by inhibiting mRNA translation. Aberrant miRNA expression profiles have been implicated in pathologies and physiological processes including pregnancy angiogenesis. Using our established model implantation failure spontaneous fetal loss pigs (Sus scrofa), 236 miRNAs were profiled compared between 1) non-pregnant pregnant endometrium, 2) maternal tissues, 3) viable growth-arrested conceptus attachment sites microarray...
Rationale: Pulmonary hypoplasia secondary to congenital diaphragmatic hernia is characterized by reduced branching morphogenesis, which responsible for poor clinical outcomes. Administration of amniotic fluid stem cell extracellular vesicles (AFSC-EVs) rescues morphogenesis in rodent fetal models pulmonary hypoplasia. Herein, we hypothesized that AFSC-EVs exert their regenerative potential affecting autophagy, a process required normal lung development. Objectives: To evaluate autophagy...
Pulmonary hypoplasia secondary to congenital diaphragmatic hernia (CDH) is characterized by impaired branching morphogenesis and differentiation. We have previously demonstrated that administration of extracellular vesicles derived from rat amniotic fluid stem cells (AFSC-EVs) rescues development hypoplastic lungs at the pseudoglandular alveolar stages in rodent models CDH. Herein, we tested whether AFSC-EVs exert their regenerative effects canalicular saccular stages, as these are...