A5049463180- Air Quality and Health Impacts
- Mitochondrial Function and Pathology
- Fungal and yeast genetics research
- Ubiquitin and proteasome pathways
- Indoor Air Quality and Microbial Exposure
- Smoking Behavior and Cessation
- ATP Synthase and ATPases Research
- Dental Anxiety and Anesthesia Techniques
- Fermentation and Sensory Analysis
- Laser Applications in Dentistry and Medicine
- Alzheimer's disease research and treatments
- Parkinson's Disease Mechanisms and Treatments
- Asthma and respiratory diseases
- Advanced Proteomics Techniques and Applications
- Carcinogens and Genotoxicity Assessment
- Dental Erosion and Treatment
- Microbial Fuel Cells and Bioremediation
- Metabolomics and Mass Spectrometry Studies
- Autophagy in Disease and Therapy
- Genomics, phytochemicals, and oxidative stress
- Photosynthetic Processes and Mechanisms
- Mass Spectrometry Techniques and Applications
- Advanced Glycation End Products research
- Advanced Chemical Sensor Technologies
- Pesticide and Herbicide Environmental Studies
University of Cologne
2017-2025
Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases
2017-2025
National Institute of Health Dr. Ricardo Jorge
2011-2021
Universidade Estadual da Paraíba
2020-2021
Centro de Genética Clínica
2013
University Medical Center Freiburg
2012
Instituto Superior Técnico
2000-2009
University of Lisbon
2009
Instituto de Biologia Experimental e Tecnológica
2003
α-Synuclein misfolding and aggregation is a hallmark in Parkinson's disease several other neurodegenerative diseases known as synucleinopathies. The toxic properties of α-synuclein are conserved from yeast to man, but the precise underpinnings cellular pathologies associated still elusive, complicating development effective therapeutic strategies. Combining molecular genetics with target-based approaches, we established that glycation, an unavoidable age-associated post-translational...
Abstract Toxicogenomics has the potential to elucidate gene–environment interactions identify genes that are affected by a particular chemical at early stages of toxicological response and establish parallelisms between different organisms. The fungicide mancozeb, widely used in agriculture, is an ethylene‐bis‐dithiocarbamate complex with manganese zinc. Exposure this pesticide been linked development idiopathic Parkinson's disease cancer. Given many signalling pathways their molecular...
Abstract Mitochondria are essential organelles and their functional state dictates cellular proteostasis. However, little is known about the molecular gatekeepers involved, especially in absence of external stress. Here we identify a role MFN2 quality control independent its function organellar shape remodeling. ablation alters proteome, marked for example by decreased levels import machinery accumulation kinase PINK1. Moreover, interacts with proteasome cytosolic chaperones, thereby...
The Saccharomyces cerevisiae SPI1 gene encodes a member of the glycosylphosphatidylinositol-anchored cell wall protein family. In this work we show results indicating that expression protects yeast from damage caused by weak acids used as food preservatives. This is documented less extended period adaptation to growth in their presence and inhibited specific rate for parental strain compared with mutant deleted. Maximal protection exerted Spi1p against equivalent concentrations various...
ABSTRACT The possible roles of 13 Msn2p- and Msn4p-regulated genes in the adaptation Saccharomyces cerevisiae to herbicide 2,4- d -dichlorophenoxyacetic acid (2,4-D) were examined. Single deletion involved defense against oxidizing agents ( CTT1 , GRX1 GRX2/TTR1 ) or encoding chaperones HSP70 family SSA1 SSA4 SSE2 showed a slight effect. A more significant role was observed for heat shock HSP78 HSP26 HSP104 HSP12 HSP42 most which encode molecular chaperones. However, SPI1 gene, member...
Cdc48/p97, a ubiquitin-selective chaperone, orchestrates the function of E3 ligases and deubiquitylases (DUBs). Here, we identify new Cdc48 in ubiquitin-dependent regulation mitochondrial dynamics. The DUBs Ubp12 Ubp2 exert opposing effects on fusion cleave different ubiquitin chains mitofusin Fzo1. We demonstrate that integrates activities these two DUBs, which are themselves ubiquitylated. First, promotes proteolysis Ubp12, stabilizing pro-fusion ubiquitylation Second, loss stabilizes...
Ubiquitin-dependent control of mitochondrial dynamics is important for protein quality and neuronal integrity. Mitofusins, fusion factors, can integrate cellular stress through their ubiquitylation, which carried out by multiple E3 enzymes in response to many different stimuli. However, the molecular mechanisms that enable coordinated responses are largely unknown. Here we show yeast Ufd2, a conserved ubiquitin chain-elongating E4 enzyme, required shape adjustments. Under various stresses,...
In a previous study, evidence was provided that indoor secondhand tobacco smoke (SHS) air pollution remains high in Lisbon restaurants where smoking is allowed, regardless of the protective measures used. The aim this study to determine these locations levels polycyclic aromatic hydrocarbons (PAH) associated with particulate phase SHS (PPAH), fraction contains recognized carginogens, such as benzo[a]pyrene (BaP). Data showed restaurant areas might contain PPAH 110 ng/m3, value significantly...
Abstract α‐synuclein (α‐syn) is the major component of Lewy bodies, a pathological hallmark Parkinson's disease and other synucleinopathies. The characterization α‐syn post‐translational modifications (PTMs), thought to interfere with its aggregation propensity cellular signaling, has been limited by availability extraction methods endogenous protein from cells tissues, antibodies toward PTMs. Here, taking advantage thermostability, we applied method achieve high enrichment soluble both...
Woody plants are particularly difficult to investigate due high phenolic, resin, and tannin contents laborious sample preparation. In particular, protein isolation from woody for two-dimensional gel electrophoresis (2-DE) is challenging as secondary metabolites negatively interfere with extraction separation. this study, three protocols, using TCA, phenol ethanol precipitation or agents, were tested in order select the more efficient recalcitrant plant gel-based proteomics. Grapevine leaves,...
Environmental tobacco smoke (ETS), also referred to as secondhand (SHS), is a major threat public health and increasingly recognized an occupational hazard workers in the hospitality industry. Therefore, several countries have implemented smoke-free regulations at industry sites. In Portugal, since 2008, legislation partially banned smoking restaurants bars but until now no data been made available on levels of indoor ETS pollution/exposure these locations. The aim this study was examine...
Mitochondria are essential organelles whose function is upheld by their dynamic nature. This plasticity mediated large dynamin-related GTPases, called mitofusins in the case of fusion between two mitochondrial outer membranes. Fusion requires ubiquitylation, attached to K398 yeast mitofusin Fzo1, occurring atypical and conserved forms. Here, modelling located ubiquitylation α4 GTPase domain, a critical helix Ras-mediated events. Structure-driven analysis revealed dual role K398. First, it...
Oral paresthesia is a localized condition of sensory abnormality that occurs in the presence injury one nerves region after certain dental procedures.The aim this study was to present case report patient who received low-level laser therapy as treatment for inferior alveolar nerve due mandibular third molar extraction surgery.A 25-year-old female reported lack sensitivity 6 months various regions bucomaxillofacial complex surgery.Laser (808 ± 10nm, 100 mW, 3J per point and 30 seconds point)...
Mitofusins are dynamin-related GTPases that drive mitochondrial fusion by sequential events of oligomerization and GTP hydrolysis, followed their ubiquitylation. Here, we show requires a trilateral salt bridge at hinge point the yeast mitofusin Fzo1, alternatingly forming before after hydrolysis. Mutations causative Charcot-Marie-Tooth disease massively map to this site, underlining relevance bridge. A triple charge swap rescues activity emphasizing close coordination residues with...
To evaluate the tooth enamel surface morphology after action of 35% hydrogen peroxide with and without LED activation.70 bovine incisors an 4x4x3 mm were used, prepared for reading superficial microhardness roughness. Specimens randomly distributed divided into 7 experimental groups (n = 10); G1 artificial saliva; G2 HP - 2 sessions (3x15´); G3 Phosphoric Acid + 3 G4 (3x15´) blue LED; G5 green G6 (3x20´) violet G7 Violet (3x20´). The results analyzed by Anova, Wilcoxon, Dunnett Tukey tests...
In this protocol we describe the separation and visualization of ubiquitylated forms yeast mitofusin Fzo1 by Western blot. To aim, express HA-tagged in Saccharomyces cerevisiae, break cells to extract a membrane-enriched fraction, solubilize membranes using detergent then specifically immunoprecipitate tagged protein anti-HA affinity beads. Subsequently, separate higher molecular weight (ubiquitylated) via SDS-PAGE. Finally, immunoblotting immunodecoration are used detect its an HA-specific...
Environmental tobacco smoke (ETS) has been recognized as a major health hazard by environmental and public authorities worldwide. In Portugal, smoke-free laws are in force for some years, banning smoking most indoor spaces. However, hospitality venues such restaurants bars, owners can still choose between total policy or partial restriction with designated areas, if adequate reinforced ventilation systems implemented. Despite that, previous study showed that workers remained continuously...