Aqueous solutions can be dispersed into picoliter droplets through a micro-channel array <italic>via</italic> centrifugation which produces monodispersed water-in-oil emulsion for digital PCR.

ABSTRACT Aggregation‐induced emission (AIE) and thermally activated delayed fluorescence (TADF) are two optoelectronic properties with great potential for applications. However, metal nanoclusters exhibiting both AIE TADF characteristics have not been extensively studied. This study investigates a binary cocrystal system based on silver nanoclusters—Ag 6 (Et 2 NCS ) ·[Ag 11 (AdmS) 3 ] ( 1‐Ag ·(Ag ), aiming to explore the synergistic effects between flexible‐alkyl dithiol rigid monothiol...

Comprehensive Summary Three three‐dimensional Hofmann‐type metal‐organic frameworks (MOFs) [Fe(bpn){Ag(CN) 2 } ]·Ph S ( 1·Ph , bpn = 1,4‐di(pyridin‐4‐yl)naphthalene, Ph diphenylsulfide), SO diphenylsulfoxide) and diphenylsulfone) were synthesized by employing sulfur‐containing aromatic guests varying in oxidation states. performed a complete four‐step spin crossover (SCO) behavior with the sequence of HS↔~LS 1/3 HS 2/3 ↔~LS 1/2 ↔ ~LS ↔LS, while an incomplete two‐step SCO profile faint at low...

Significance Digital PCR (dPCR) has long been recognized as a powerful tool in nucleic acid quantitative analysis, but there still remain problems that hinder its wide popularity. Sample loss is one major challenge compromises the certainty of dPCR quantification and handicaps ability to detect quantify samples low copy number. Another main difficulty prevent this single-copy-sensitivity experiment from possible contaminations product exposure. The CLEAR-dPCR introduced herein solves these...

we incorporated redox-active tetrathiafulvalene (TTF) and tetrachloroperylene dianhydride (TCPDA) into a cocrystal its effective NIR-II photothermal which results from the twisted conformation was characterized analysed.

Abstract Photogenerated radicals are an indispensable member of the state‐of‐the‐art photochromic material family, as they can effectively modulate photoluminescence and photothermal conversion performance radical‐induced complexes. Herein, two novel metal–organic frameworks (MOFs), [Ag(TEPE)](AC) ⋅ 7 / 4 H 2 O 5 EtOH ( 1 ) [Ag(TEPE)](NC) 3H ), reported. Distinctly different topological networks be obtained by judiciously introducing alternative π‐conjugated anionic guests, including a new...

This work describes a use of composite nanomaterial which consists multiwall carbon nanotubes covered by iron oxide nanoparticles as hybrid electrode in aqueous supercapacitor. The investigated was manufactured two-step simple chemical synthesis the first step functionalization whereas second one deposition oxide. According morphological and structural characterization, with diameters 10–40 nm were successfully randomly-dispersed magnetite average diameter 10 nm. Moreover, thermogravimetric...

Polyoxometalates (POMs) with various coordination fashions are versatile ligands for constructing single-ion magnets (SIMs), but enforcing POM-SIMs a specific geometry remains synthetic challenge. Herein, we synthesized POM-cocrystallized DyIII–SIM [Dy(OPPh3)4(H2O)3][PW12O40]·4EtOH (1Dy) and POM-ligated [{Dy(OPPh3)3(H2O)3}{PW12O40}]·Ph3PO·H2O (2Dy) pentagonal bipyramidal local geometry. Magnetic measurements indicate that 1Dy displays field-induced single-molecule magnet (SMM) behavior the...

Two magic number Au 13 nanoclusters were tailored using two N-containing multidentate phosphine ligands with a classical icosahedral core and an unprecedented “UFO”-shaped polyhedral core, respectively.

Organic photothermal conversion materials hold immense promise for various applications owing to their structural flexibility. Recent research has focused on enhancing near-infrared (NIR) absorption and mitigating radiative transition processes. In this study, we have developed a viable approach the design of through construction ternary organic cocrystals, by introducing third component as molecular blocker motion unit into binary donor-acceptor system. Superstructural photophysical...

Digital polymerase chain reaction (PCR) is becoming ever more recognized amid the overwhelming revolution in DNA quantification, genomics, genetics, and diagnostics led by technologies such as next generation sequencing studies at single-cell level. The demand to quantify amount of RNA has been driven molecular level digital PCR, with its unprecedented quantification capability, sure shine coming era. Two decades ago, it emerged a concept; yet one decade integration microfluidics invigorated...

A systematic survey of the oil and surfactant components stable monodisperse w/o droplets suitable for various methods.

Droplets generated by centrifugation can be packed as a thin layer flipping micro-centrifugal tube with plastic insert. RS-dLAMP applies line-scan to enable high-speed and contamination-free digital quantification of nucleic acids.

Sebocyte differentiation is a continuous process, but its potential molecular mechanism remains unclear. We aimed to establish novel sebocyte model using human primary sebocytes and identify the expression profiles of differentiation-associated proteins. Primary were cultured on Sebomed medium supplemented with 2% serum for 7 days. Flow cytometry showed that S phase cells decreased time-dependently, while G1 subG1 (apoptosis) increased under starvation. Transmission electron microscopy Oil...

Sperm-associated antigen 5 (SPAG5) has been identified as a driver in several type of cancers. In this study, we aimed to reveal the role SPAG5 melanoma and clarify whether FOXM1 (forkhead box protein M1) /ADAM17 (A disintegrin metalloproteinase 17) /NOTCH1 signaling was involved. The expression malignant (MM) tissues matched normal detected using qRT-PCR, immunohistochemistry Western blotting. Cell viability tested CCK-8 (Cell Count Kit-8), colony formation EdU staining. migration...

Abstract Photogenerated radicals are an indispensable member of the state‐of‐the‐art photochromic material family, as they can effectively modulate photoluminescence and photothermal conversion performance radical‐induced complexes. Herein, two novel metal–organic frameworks (MOFs), [Ag(TEPE)](AC) ⋅ 7 / 4 H 2 O 5 EtOH ( 1 ) [Ag(TEPE)](NC) 3H ), reported. Distinctly different topological networks be obtained by judiciously introducing alternative π‐conjugated anionic guests, including a new...

Abstract Organic photothermal conversion materials hold immense promise for various applications owing to their structural flexibility. Recent research has focused on enhancing near‐infrared (NIR) absorption and mitigating radiative transition processes. In this study, we have developed a viable approach the design of through construction ternary organic cocrystals, by introducing third component as molecular blocker motion unit into binary donor–acceptor system. Superstructural...

Abstract The realization of the vast potential digital PCR (dPCR), to provide extremely accurate and sensitive measurements in clinical setting, has thus far been hindered by challenges such as assay robustness high costs. Existing popular dPCR platforms that target clinic have not reached wide-spread adoption, due problems with sample loss risk contamination during preparation, compartmentalization, transfers; limitations dynamic range signal-to-noise result readout also restricts broad...

Background and Aims: We conducted a study on mice to investigate how osteopontin, mediator of cell-cell cell-extracellular matrix (ECM) communication invasion, influences embryonic development, metabolism, implantation potential, with confirmation by 3D in-vitro model. Method: Embryos were collected from female cultured under different conditions: HTF only, supplemented OPN at doses, OPN-supplemented anti-[Formula: see text]v[Formula: text]3 Ab or RGS2 inhibitors. Embryo morphology, rates...