A5102737000- Anesthesia and Pain Management
- Developmental Biology and Gene Regulation
- Cardiac, Anesthesia and Surgical Outcomes
- Anesthesia and Sedative Agents
- Pain Mechanisms and Treatments
- Gastrointestinal Tumor Research and Treatment
- Wnt/β-catenin signaling in development and cancer
- Spine and Intervertebral Disc Pathology
- Congenital heart defects research
- Airway Management and Intubation Techniques
- RNA Research and Splicing
- Gastric Cancer Management and Outcomes
- Dental Anxiety and Anesthesia Techniques
- Muscle metabolism and nutrition
- Renal and related cancers
- Pluripotent Stem Cells Research
- Cardiovascular Issues in Pregnancy
- Nausea and vomiting management
- Zebrafish Biomedical Research Applications
- Cellular Mechanics and Interactions
- Pain Management and Treatment
- Kruppel-like factors research
- Hippo pathway signaling and YAP/TAZ
- Uterine Myomas and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
Sapporo Medical University
2016-2025
Shanghai Institute for Science of Science
2025
Institute of Science Tokyo
2025
Yokohama City University
2022-2024
Yokohama City University Medical Center
2022-2024
Yokosuka Kyosai Hospital
2023
VA San Diego Healthcare System
2017-2022
University of California, San Diego
2016-2022
Yokohama City University Hospital
2022
Washington University in St. Louis
2013-2018
The temporal and spatial regulation of somitogenesis requires a molecular oscillator, the segmentation clock. Through Notch signalling, oscillation in cells is coordinated translated into cyclic wave expression hairy-related other genes. sweeps caudorostrally through presomitic mesoderm (PSM) finally arrests at future point anterior PSM. By experimental manipulation analyses zebrafish mutants, we have found novel component involved this process. We report that level Fgf/MAPK activation...
Four members of the TEAD/TEF family transcription factors are expressed widely in mouse embryos and adult tissues. Although vitro studies have suggested various roles for TEAD proteins, their vivo functions remain poorly understood. Here we examined role Tead genes by generating mutants Tead1 Tead2. Tead2(-/-) mice appeared normal, but Tead1(-/-); died at embryonic day 9.5 (E9.5) with severe growth defects morphological abnormalities. At E8.5, were already small lacked characteristic...
The telencephalon is formed in the most anterior part of central nervous system (CNS) and organised into ventral subpallial dorsal pallial domains. In mice, it has been demonstrated that Fgf signalling an important role induction patterning telencephalon. However, precise still unclear, owing to overlapping functions family genes. To address this, we have examined, zebrafish embryos, activation Ras/mitogen-activated protein kinase (MAPK), one major downstream targets signalling....
BackgroundMicroglia of the central nervous system act as sentinels and rapidly react to infection or inflammation. The pathophysiological role bone marrow-derived microglia is particular interest because they affect neurodegenerative disorders neuropathic pain. hypothesis current study that chronic psychological stress (chronic PS) induces infiltration into hypothalamus by means chemokine axes in brain marrow. Methods FindingsHere we show specifically infiltrate paraventricular nucleus (PVN)...
Summary Bone marrow–derived microglia aggregated in the central nucleus of amygdala are involved chronic neuropathic pain–induced anxiety. Chronic pain causes abnormal sensitivities such as hyperalgesia and allodynia, emotional abnormalities anxiety depression. Although spinal cord sensitivity to pain, no previous studies have examined mechanism Here, we involvement bone marrow (BM)–derived amygdalae mice with development anxiety-like behavior. We prepared partial sciatic nerve ligations...
There is still no consensus on the analgesic range and mechanisms of action modified thoracoabdominal nerve block through perichondrial approach (M-TAPA). This cadaveric study aimed to determine spread an injectate following simulated M-TAPA.Simulated M-TAPA injections (n=8) were administered both sides soft embalmed Thiel cadavers with 25 mL a saline-soluble dye. Anatomic dissection was performed document staining (deeply, faintly, or not stained) anterior cutaneous branches nerves extent...
Dachsous (Dchs), an atypical cadherin, is evolutionarily conserved regulator of planar cell polarity, tissue size, and adhesion. In humans, DCHS1 mutations cause pleiotropic Van Maldergem syndrome. Here, we report that in zebrafish dchs1b dchs2 disrupt several aspects embryogenesis, including gastrulation. Unexpectedly, maternal zygotic (MZ) mutants show defects the earliest developmental stage, egg activation, abnormal cortical granule exocytosis (CGE), cytoplasmic segregation, cleavages,...
ABSTRACT Background Immunocompromised individuals are infected with persistent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and often refractory to treatment. Case Summary A 56-year-old woman undergoing chemotherapy for follicular lymphoma experienced relapsing pulmonary infiltrates a consistently low cycle threshold value of nasal swab SARS-CoV-2 PCR over 5-month period. Despite repeated remdesivir glucocorticoid therapy, her symptoms worsened progressed failure. Viral...
Interventions that preserve motor neurons or restore functional neuroplasticity may extend longevity in amyotrophic lateral sclerosis (ALS). Delivery of neurotrophins potentially revive degenerating neurons, yet this approach is dependent on the proper subcellular localization neurotrophin receptor (NTR) to plasmalemmal signaling microdomains, termed membrane/lipid rafts (MLRs). We previously showed over-expression synapsin-driven caveolin-1 (Cav-1) (SynCav1) increases MLR NTR [e.g.,...
The cell population and the activity of organizer change during course development. We addressed mechanism mouse node development via an analysis node/notochord enhancer (NE) Foxa2. first identified core element (CE) enhancer, which in multimeric form drives gene expression node. CE was activated Wnt/β-catenin-treated P19 cells with a time lag, this activation dependent on two separate sequence motifs within CE. These same were also required for transgenic embryos. Tead family transcription...
Studies in vitro and vivo demonstrate that membrane/lipid rafts caveolin (Cav) organize progrowth receptors, and, when overexpressed specifically neurons, Cav-1 augments neuronal signaling growth improves cognitive function adult aged mice; however, whether overexpression can preserve motor the brain trauma setting is unknown. Here, we generated a neuron-targeted Cav-1–overexpressing transgenic (Tg) mouse [synapsin-driven (SynCav1 Tg)] subjected it to controlled cortical impact model of...
Abstract Anteroposterior (AP) axis extension during gastrulation requires embryonic patterning and morphogenesis to be spatiotemporally coordinated, but the underlying genetic mechanisms remain poorly understood. Here we define a role for conserved chromatin factor Gon4l, encoded by ugly duckling ( udu ), in coordinating tissue zebrafish through direct positive negative regulation of gene expression. Although identified as recessive enhancer impaired planar cell polarity (PCP) mutants,...
The fetal liver kinase 1 (FLK-1)(+) hemangioblast can generate hematopoietic, endothelial, and smooth muscle cells (SMCs). ER71/ETV2, GATA2, SCL form a core transcriptional network in development. Transient coexpression of these three factors during mesoderm formation stage mouse embryonic stem (ESCs) robustly enhanced generation by activating bone morphogenetic protein (BMP) FLK-1 signaling while inhibiting phosphatidylinositol 3-kinase, WNT signaling, cardiac output. Moreover, etsrp,...
Elevating neuroprotective proteins using adeno-associated virus (AAV)-mediated gene delivery shows great promise in combating devastating neurodegenerative diseases. Amyotrophic lateral sclerosis (ALS) is one such disease resulting from loss of upper and lower motor neurons (MNs) with 90-95% cases sporadic (SALS) nature. Due to the unknown etiology SALS, interventions that afford neuronal protection preservation are urgently needed. Caveolin-1 (Cav-1), a membrane/lipid rafts (MLRs)...
We applied mobile computing to community support and explored with a large number of terminals. This article reports on the Second International Conference Multiagent Systems (ICMAS'96) Mobile Assistant Project that was conducted at an actual international conference for multiagent systems using 100 personal digital assistants (PDAs) cellular telephones. supported three types service: (1) communication services such as e-mail net news; (2) information conference, personal, tourist...
We have previously reported on the possibility that light resistance exercise performed with a high plasma amino acid concentration resulting from ingestion of high-protein snack (HPS; 15 g protein, 18 sugar) 3 h after basal meal promotes utilization acids in peripheral tissues such as muscle both rats and humans. In present study, we further examined effectiveness daily routine involving HPS subsequent (dumbbell exercise) increasing mass strength human muscle. Ten young adult males were...
A harmful effect of stress hormone secretion during surgery is lipolysis and proteolysis to maintain normal blood glucose levels. well-titrated general anesthetic improves control by suppressing these hormones. The aim this study was explore the intraoperative infusion on in patients undergoing a consisting sevoflurane remifentanil long (>6 hours) major surgery.In prospective, single-blinded, randomized, multicenter trial, 80 with an expected duration anesthesia >6 hours were allocated...