Pseudoexfoliation (PEX) syndrome is a systemic disorder of the elastic fiber system that can lead to PEX glaucoma. Elastotic alterations in lamina cribrosa (LC) eyes suggested biomechanical implications predisposing pressure-induced optic nerve damage. In this pilot study, stiffness LC and peripapillary sclera (ppSC) with without were analyzed by atomic force microscopy (AFM) nanoindentation.Unfixed cryosections (5-μm thick) prepared from heads (ONH) three donor age-matched control eyes. AFM...

Riboflavin/UVA-induced corneal collagen cross-linking has become an effective clinical application to treat keratoconus and other ectatic disorders of the cornea. Its beneficial effects are attributed a marked stiffening unphysiologically weak stroma. Previous studies located effect predominantly within anterior In this study, we present atomic force microscopy-derived analysis depth-dependent distribution Young's modulus with depth resolution 5 µm in 8 cross-linked porcine corneas...

Abstract Der Eindruck von Leichtigkeit und Transparenz durch die Verwendung Glas in Gebäuden führt zu einem stetig wachsenden Einsatz der Fassade. Gleichzeitig steigen aber auch Anforderungen an den Wärmeschutz sowie das Bedürfnis nach Sicherheit Nutzer. Dem Stand Technik entspricht Dreischeiben‐Isoliergläsern, Verbindung mit zusätzlichen Schichten aktive passive Sicherheitsanforderungen erfüllen. Allerdings resultieren daraus ein deutlich höheres Eigengewicht größere Gesamtdicken...

Abstract This study evaluates the corneal applicability of a refractive ultraviolet femtosecond laser in postmortal human donor eyes and ex vivo porcine eyes. Refractive lenticule extraction flap creation were attempted 10 80 with without abrasion epithelium. The postmortem interval ranged from 6 to 35 h samples was set 4, 24, 48 for specimens. Nine 60 treated an laser. rest infrared Optical coherence tomography scanning electron microscopy used demonstrate success or failure procedures....

To examine the effect of a vortex beam on gas formation and interface quality after keratorefractive lenticule creation with 347 nm UV femtosecond laser. "masked for peer-review". Experimental study. Refractive corneal lenticules were created in 40 ex vivo porcine eyes In 20 eyes, laser was operated (Laguerre-Gaussian) (generated by spiral phase plate), remaining normal (Gaussian) beam. each group, 12 used histological quantification intraoperative formation. The 8 subjected to scanning...

The intention of this observational study is to show the significant impact comorbidities and smoking on outcome in aneurysmal subarachnoid hemorrhage (SAH). During 203 cases treatment ruptured intracranial aneurysms were analyzed. We examined classified prospectively 12 month according modified Rankin Scale (mRS) considering retrospectively a history investigated occurrence early delayed cerebral ischemia between 2012 2017. Using logistic regression methods, we revealed (odds ratio 0.21; p...

To analyze morphologic changes in the trabecular meshwork (TM) of bovine eyes treated with topical prednisolone and exhibiting elevated intraocular pressure for 4 weeks.The TM four adult Braford cow 0.5% eye drops three times daily 7 weeks their contralateral artificial tear preparation that two untreated cows young calves were analyzed light electron microscopy. Increased extracellular matrix (ECM) under outflow loops was evaluated quantitatively. Additionally, deparaffinized tissue labeled...

Burnout-Beschwerden sind ein bereits früh bei Medizinstudierenden auftretendes Problem. Medizinstudierende haben Berufsstart meist einen guten Gesundheitsstatus, der sich jedoch im Verlauf dramatisch verschlechtert. Im Rahmen unserer ESTRELLAS Studie untersuchten wir insgesamt 530 Studierende vorklinischen Studienabschnitt Humanmedizin bis zum 1. Staatsexamen unter Anwendung validierter psychologischer Fragebögen. des Studienabschnitts weisen stetig mehr Risikomuster Arbeits- und...

life (7.35 per 100,000 year)(4).Overall, males are affected approximately twice as often females (2,25).In the 8 th decade of life, however, a male:female ratio about 4:1 is observed.Pathologically, cSDH represents an accumulation blood between dura mater and arachnoid mater.It displays different conditions liquefaction usually surrounded by more or less pronounced fibrin capsule, which forms 2-3 weeks after primary intracranial injury associated with acute subdural hematoma...

Purpose: To analyze morphological changes in trabeculectomy specimens of patients suffering from uveitic secondary open angle glaucoma (UG).Material and Methods: Trabeculectomy six uveitis for 2 to 19 years having been treated with steroids 5 86 months were obtained during antiglaucomatous surgery after failed drug treatment. Six specimens, four Fuchs' heterochromic cyclitis (FHC), one Herpes simplex-associated (HS), juvenile idiopathic arthritis-associated (JIA) analyzed by light electron...

The aim of this study was to assess the biomechanical efficacy transepithelial collagen crosslinking using femtosecond laser pocket technique compared with that standard (CXL) technique.Forty ex vivo porcine eyes were divided into 4 groups 10 samples each. Group 1 comprised untreated controls. 2 CXL group debridement, instillation 0.1% riboflavin-dextran solution for 15 minutes before and every 5 during 30 irradiation ultraviolet A (UVA) light 370 nm an irradiance 3 mW/cm². pertained...

The study aimed to characterize the expression and function of SFTA3 at ocular surface in tears. Ocular tissues, conjunctival (HCjE) human corneal (HCE) epithelial cell lines as well tearfilm patients suffering from different forms dry eye disease (DED) were analyzed by means RT-PCR, western blot, immunohistochemistry, ELISA. A possible role recombinant wound healing was investigated performing vitro scratch assays. Tear film regulatory properties with spinning drop method regulation...

To demonstrate the capacity of interleukin (IL)-1 to simultaneously lower intraocular pressure (IOP) and induce trabecular ELAM-1 expression in one experiment test for IL-6 accordingly evaluate role transforming growth factor (TGF)-β2 as an IL-1 antagonist.Forty-two porcine eyes were subjected meshwork (TM) perfusion with IL-1α, -1β, or -6 48 hours. Twelve IL-1α-treated also received TGF-β2 final 24-hour period. Polymerase chain reaction Western blot analyses then performed on harvested TM...

This prospective study is designed to detect changes in the treatment of ruptured intracranial aneurysms over a period 17 years.We compared 361 treated cases aneurysm occlusion after subarachnoid hemorrhage from 1997 2003 with 281 2006 2014. Specialists neuroradiology and vascular neurosurgery decided modality assignment. We established data acquisition both groups significant differences within follow-up time one year. With this setting we evaluated methods endovascular microsurgical...

The aqueous humor (AH) component transforming growth factor (TGF)-β2 is strongly correlated to primary open-angle glaucoma (POAG), and was shown up-regulate glaucoma-associated extracellular matrix (ECM) components, members of the ECM degradation system heat shock proteins (HSP) in ocular cells. Here we present osteopontin (OPN) as a new TGF-β2 responsive cultured human optic nerve head (ONH) astrocytes. Activation initially demonstrated by Oligo GEArray microarray confirmed semiquantitative...

Purpose: To establish a simplified three-dimensional (3D) meibomian gland culture model using epithelial cell (HMGEC) line that might be useful tool to gain deeper insights into dysfunction. For this purpose, 3D differentiation patterns and growth characteristics of HMGECs were studied on various membranes/scaffolds as well in hanging drops. Methods: Several types inserts consisting different materials (Millicell-HA, Millicell-PCF, ThinCert, Alvetex) drop analyzed. Culture conditions...

Purpose To assess the suitability of a new 345 nm ultraviolet (UV) femtosecond laser for refractive surgery. Setting Department Ophthalmology, University Erlangen-Nürnberg, Erlangen, Germany. Design Experimental study. Methods Twenty-five porcine corneas were used stromal flap or lamellar bed creation (stromal depth, 150 μm) and 15 rabbit near endothelium. Ultraviolet cutting-line morphology, gas formation, keratocyte death rate evaluated using light electron microscopy compared with...