A5101921839- Circular RNAs in diseases
- Gene expression and cancer classification
- Cell Image Analysis Techniques
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Image Processing Techniques and Applications
- Genomics and Chromatin Dynamics
- MicroRNA in disease regulation
- Esophageal Cancer Research and Treatment
- Medical Imaging Techniques and Applications
- Single-cell and spatial transcriptomics
- Advanced Fluorescence Microscopy Techniques
- RNA Research and Splicing
- Tuberculosis Research and Epidemiology
- Cancer-related gene regulation
- Antibiotic Resistance in Bacteria
- Wnt/β-catenin signaling in development and cancer
- Viral-associated cancers and disorders
- Phytochemical compounds biological activities
- Genetics and Neurodevelopmental Disorders
- Molecular Biology Techniques and Applications
- RNA and protein synthesis mechanisms
- Renal and Vascular Pathologies
- Advanced Proteomics Techniques and Applications
- Toxin Mechanisms and Immunotoxins
University of California, Davis
2022-2024
Beijing Normal University
2017-2023
Jilin University
2021-2022
Shanghai Medical College of Fudan University
2020
Shandong First Medical University
2008-2009
Shandong Tumor Hospital
2008-2009
Abstract The field of single-cell transcriptomics has been producing extensive datasets, advancing our understanding cellular functions in various tissues, and empowering diagnosis, prognosis, drug development. However, parsing through this data a monumental task, often stretching weeks to months. This bottleneck arises due the sheer volume generated—ranging from hundreds gigabytes tens terabytes—that demands time for analysis. Moreover, analysis involves an intricate series steps utilizing...
As a measure of cytotoxic potency, half-maximal inhibitory concentration (IC50) is the at which drug exerts half its maximal effect against target cells. It can be determined by various methods that require applying additional reagents or lysing Here, we describe label-free Sobel-edge-based method, name SIC50, for evaluation IC50. SIC50 classifies preprocessed phase-contrast images with state-of-the-art vision transformer and allows continuous assessment IC50 in faster more cost-efficient...
Abstract Dysregulation of wingless‐type (Wnt) signaling is implicated in hepatocellular carcinoma (HCC). Wnt family member 8B (Wnt8B), one the canonical ligands, oncogenesis. However, role Wnt8B human HCCs and its transcriptional regulation mechanism are presently unknown . Here, we report that expression was frequently increased significantly associated with poorer patient prognosis. knockdown suppresses HCC cell growth both vitro vivo via inhibiting signaling. Zinc finger transcription...
The fatty acid synthase type II (FAS-II) multienzyme system is the main target of drugs to inhibit mycolic synthesis in mycobacterium. Meromycolate extension acyl carrier protein (AcpM) serves as chain shuttling among individual FAS-II components during progression elongation. In this paper, MSMEG_5634 Mycobacterium smegmatis was determined be a helix-grip structure with deep hydrophobic pocket, preferring form complex acyl-AcpM containing at C36-52 length, which medium product FAS-II....
Abstract Noncoding RNAs (ncRNAs), including microRNAs (miRNAs) and circular (circRNAs), which are expressed with a daily rhythm in the rat pineal gland, associated regulation of melatonin secretion other biological functions. However, mechanisms these molecules gland not yet fully understood. In this study, we found that circR-WNK2 was highly at night, may be involved through competitive endogenous RNA (ceRNA) mechanism. By dual luciferase reporter, pull-down, fluorescence situ hybridization...
Abstract Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is a powerful analytical tool that enables the visualization and comparison of relative abundances metabolites across samples, shedding light on biological processes disease mechanisms. Techniques such as scSpatMet enable determination cell boundaries types through staining with 35 marker antibodies. Yet, distinguishing subpopulations cells, astrocytes, oligodendrocytes, neuronal clusters in brain,...
Large language models, when integrated into bioinformatics tools, offer valuable support for biologists in the analysis of single-cell transcriptomic data. Here, we introduce Bioinformatics Copilot 2.0, an advanced tool that builds upon its predecessor with four significant enhancements: 1) Internal server processing: users can now process large datasets on internal server, thereby ensuring data privacy. 2) User-controlled analysis: take control their tasks exceed copilot's capabilities. 3)...
CircRNAs have been identified to be expressed differently and stably in numerous species tissues, but their functions growth hormone (GH) secretion are still largely unknown. In summary, we revealed a circRNA-miRNA-mRNA network that may play biological role the rat pituitary gland. First, verified chromosome location information of circAgtpbp1 according sequencing analysis. The characteristics were authenticated through PCR, qRT–PCR, treating with RNase fluorescent situ hybridization (FISH)....
e15665 Background: To determine the optimal method of using 3-deoxy-3- 18 F-fluorothymidine (FLT) positron emission tomography (PET) to estimate gross tumor length in esophageal carcinoma, and compared with that F- fluorodeoxyglucose(FDG) PET. Methods: Twenty patients squamous cell carcinoma treated radical surgery were enrolled detected by FLT PET, eighteen them underwent FDG PET scan. Gross volumes (GTVs) delineated seven different methods PET: visual interpretation, standardized uptake...
Abstract As a measure of cytotoxic potency, half-maximal inhibitory concentration (IC50) is the at which drug exerts half its maximal effect against target cells. It can be determined by various methods that require applying additional reagents or lysing Here, we describe label-free Sobel-edge-based method, name SIC50, for evaluation IC50. SIC50 classifies pre-processed phase-contrast images with state-of-art vision transformer and allows continuous assessment IC50 in faster more...