A5101662691- Animal Genetics and Reproduction
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Reproductive Biology and Fertility
- Chromosomal and Genetic Variations
- Molecular Biology Techniques and Applications
- Genomics and Chromatin Dynamics
- MicroRNA in disease regulation
- Animal Nutrition and Physiology
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sperm and Testicular Function
- Pharmacological Effects of Natural Compounds
- Xenotransplantation and immune response
- Epigenetics and DNA Methylation
- China's Socioeconomic Reforms and Governance
- Reproductive System and Pregnancy
- Aquaculture Nutrition and Growth
- Wnt/β-catenin signaling in development and cancer
- Reproductive biology and impacts on aquatic species
- Circular RNAs in diseases
- Educational Technology and Assessment
- RNA Research and Splicing
Yangzhou University
2013-2025
Jiangsu University of Science and Technology
2022-2025
Ministry of Agriculture and Rural Affairs
2023
Huzhou Academy of Agricultural Sciences
2007
Insulin is an important component of stem cell cultures; however, its role in the proliferation avian primordial germ cells (PGCs) unknown. The PGCs cultures varies and growth factors signaling pathways necessary to induce chickens are Therefore, we conducted present study investigate effect insulin on survival PGCs. In this study, observed that under culture system, proliferate presence insulin, but do not absence insulin. Furthermore, insulin-lacking media, expression pluripotency genes,...
To study self-renewal, genetic modification, and differentiation of avian spermatogonial stem cells (SSCs), we isolated chicken SSCs from fetal testes on the 16th hatching day via enzyme digestion, then cultured over 2 months after purification in vitro. were identified by alkaline phosphatase staining SSEA-1 fluorescence. The EGFP gene was transfected into three different methods: electroporation, liposome transfer calcium acid phosphate precipitation. transfection rate cell survival using...
Abstract In this study, we investigated the mechanism of signaling pathway‐mediated differentiation embryonic stem cells (ESCs) into spermatogonial (SSCs) in chicken. The Wnt pathway was identified based on previous RNA Sequencing results and proven a crucial that participates ESCs SSCs. retinoic acid (RA) induction experiments vitro, found expression inhibited by Wnt5a‐shRNA, resulting decreased corresponding marker genes SSCs, C‐kit, Cvh, integrin α6 β1, but it significantly promoted RA...
The objectives of the present study were to screen for key gene and signaling pathways involved in production male germ cells poultry investigate effects transforming growth factor beta (TGF-β) pathway on differentiation chicken embryonic stem (ESCs) into cells. ESCs, primordial cells, spermatogonial (SSCs) sorted using flow cytometry RNA sequencing (RNA-seq) technology. Male ESCs induced 40 ng/mL bone morphogenetic protein 4 (BMP4). TGF-β SSCs confirmed by morphology, quantitative real-time...
ABSTRACT Stra8 (stimulated by retinoic acid gene 8) is a specific that expressed in mammalian germ cells during transition from mitosis to meiosis and plays key role the initiation of mammals birds. So, evaluation pathway cSSCs may provide deeper insight into spermatogenesis. miRNA was also an important regulating factor for SSCs. However, there currently no data indicating regulate SSCs via Stra8. Here, we predicted prospective targeting using online Bioinformatics database‐Targetscan,...
Spermatogonial stem cells (SSCs) form the foundation for spermatogenesis and sustain male fertility. To explore regulatory mechanisms of chicken SSCs generation, we obtained highly purified embryonic (ESCs), primordial germ (PGCs) by fluorescence-activated cell sorting (FACS). High-throughput analysis methods (RNA-Seq) were used to sequence transcriptome level these cells. Gene ontology Kyoto encyclopedia genes genomes (KEGG) pathway enrichment analyze RNA-Seq results. BMP4 was induce ESCs...
Abstract The study aims to analyze the key signaling pathways in regulating process of embryonic stem cells (ESCs) differentiation into spermatogonial (SSCs). We explored specific mechanisms C‐Jun amino‐terminal kinase (JNK) this process. Interference/overexpression MAPK8 allows JNK pathway be blocked/activated. In Retinoic acid (RA) induced vitro assays, expression germ cell marker genes, cvh, c‐kit, integrin α6 and β1 , was observed upregulate while activating significantly. Fluorescence...
Several inducers have been used to differentiate embryonic stem cells (ESCs) into male germ but the induction process has inefficient. To solve problem of low efficiency inducer for ESCs differentiation cells, all-trans retinoic acid (ATRA), Am80(the receptor agonist), and estradiol (E2) was induce in vitro. were cultured media containing ATRA, Am80, or E2 respectively which can a cell lineage. In ATRA Am80 Group, cell-like be observed 10 days; no Group. The marker genes cell: Dazl, Stra8,...
The experiment was conducted to study the development of ovary in quails’ embryos which were incubated for 4 17 days and out 1 day. or gonads cut HE staining carried out. results showed that when embryo hatched days, lots primordial germ cells (PGCs) clustered region where gonad would be formed. On 5th day hatching, began formed exhibited feature testis. 7th hatching day, right degenerate, just a few PGCs differentiate into oogonia. 10th there many oogonia ovary, some surrounded by other...
Cherry Valley ducks (CVDs) and White Kaiya (WKDs) are judged to be fast- slow-growing lines, respectively. To investigate the carcass traits nutritional profile at their marketable ages, 12 birds (38 days for CVDs, n = 6; 56 WKDs, 6) were randomly selected slaughtered. Indicators such as breast muscle weight, shear force, proximate composition comprehensively detected. Although weight in WKDs significantly lower, remarkably higher intramuscular fat tenderness less moisture content observed...
The production of germ cells, especially primordial cells (PGCs), is important for avian stem and reproduction biology. However, key factors involved in the regulation PGCs remain unknown. Here, we report a PGC-related marker gene: C1EIP (Chromosome 1 Expression PGCs), whose activation expression are regulated by transcription factor STAT3 (signal transducer activator 3), histone acetylation, promoter methylation. regulates formation mediating PGC-associated genes, such as CVH (Chicken Vasa...
In biological research, chicken embryos are a classic experimental model for the exploration of embryonic development and cell differentiation. Transferring exogenous substances into producing medical antibodies has been widely used in production practice. However, there few studies about effect different injection site dosage on embryos. The aim this study was to explore effects sites dosages embryo hatching rate development, so as provide basis further using model. Freshly laid eggs (Rugao...
Lin28A and Lin28B are homologous RNA-binding proteins that participate in the development of primordial germ cells. The mechanisms underlying expression regulation have been well documented, but such information for is limited. In this study, a fragment promoter was cloned, pEGFP-pLin28B vector constructed. DF-1 chicken fibroblasts were transfected green fluorescent protein (GFP) measured. Furtherly, different lengths fragments cloned using chromosome-walking method ligated into PGL3-Basic...
Interleukin-10 (IL-10) has been recently identified as a multifunctional cytokine, because of its close link with immunoregulation and anti-inflammatory responses. This study investigated the association IL-10 genetic polymorphisms immune traits New Zealand white rabbits (N-W), Fujian yellow (F-Y) their reciprocal crosses (N-Y Y-N, respectively). SNPs on five exons gene were genotyped in 204 healthy via PCR-SSCP DNA sequencing. Two (A1435G G1519A, both synonymous mutations) six genotypes...
As a classical model system of embryo biology, the chicken has been used to investigate embryonic development and differentiation. Delivering exogenous materials into embryos great advantage for studying gene function, transgenic breeding, chimera preparation during development. Here we show method in ovo intravascular injection whereby such as plasmid vectors or modified primordial germ cells (PGCs) can be transferred donor at early developmental stages. The results that through dorsal...