A5014541123- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Helicobacter pylori-related gastroenterology studies
- Plant-Microbe Interactions and Immunity
- Traditional Chinese Medicine Studies
- Acupuncture Treatment Research Studies
- Advanced Glycation End Products research
- Plant Disease Resistance and Genetics
- Neurological Disease Mechanisms and Treatments
- Gut microbiota and health
- Advanced Computational Techniques and Applications
- Immune Response and Inflammation
- Pharmacological Effects of Natural Compounds
- Network Security and Intrusion Detection
- Traditional Chinese Medicine Analysis
- Bladder and Urothelial Cancer Treatments
- T-cell and Retrovirus Studies
- Plant Pathogens and Fungal Diseases
- Parasitic Infections and Diagnostics
- Seaweed-derived Bioactive Compounds
- Polysaccharides and Plant Cell Walls
- interferon and immune responses
- Hernia repair and management
- Pain Mechanisms and Treatments
- Marine Sponges and Natural Products
Shanghai Institute of Technology
2022-2025
Capital Medical University
2011-2024
Beijing Tian Tan Hospital
2024
Ocean University of China
2024
Sichuan University
2024
West China Hospital of Sichuan University
2024
Chengdu University
2024
Shandong University
2005-2023
Hunan University of Technology
2023
Shanxi University
2002-2023
Generation of functional spermatids from human spermatogonial stem cells (SSCs) in vitro is utmost importance for uncovering mechanisms underlying germ cell development and treating infertility. Here we report a three-dimensional-induced (3D-I) system by which SSCs were efficiently differentiated into haploid spermatids. Human isolated identified phenotypically. Meiotic chromatin spreads DNA content assays revealed that spermatocytes effectively generated 3D-I system. Haploid derived...
Effect of berberine on hyperglycaemia and gut microbiota composition in type 2 diabetic Goto-Kakizaki rats
Spermatogonial stem cells (SSCs) can differentiate into spermatids, reflecting that they could be used in reproductive medicine for treating male infertility. SSCs are able to become embryonic stem-like with the potentials of differentiating numerous cell types three germ layers and transdifferentiate mature functional other lineages, highlighting significant applications human diseases. However, very rare a long-term culture system has not yet established. This aim study was isolate,...
Spermatogonial stem cells (SSCs) have significant applications in both reproductive and regenerative medicine. However, primary human SSCs are very rare, a SSC line has not yet been available. In this study, we for the first time reported stable by stably expressing SV40 large T antigen. RT-PCR, immunocytochemistry, Western blots revealed that cell was positive number of spermatogonial hallmarks, including VASA, DAZL, MAGEA4, GFRA1, RET, UCHL1, GPR125, PLZF THY1, suggesting these...
<i>Coprinus comatus</i> polysaccharide (CCP) has significant hepatoprotective effect. To explore mechanism of CCP, the study analyzed preventive effect CCP on acute alcoholic liver injury in mice by histopathological examination and biochemical analysis. Simultaneously, was also conjunction with metabolomics proliferation gut microbiota. The results showed that significantly decreased alanine aminotransferase (ALT), aspartate (AST) triglyceride (TG) levels serum disease (ALD) mice....
ABSTRACT High concentrations of pigment powders present significant challenges for dispersion stability in cosmetic formulations, the iron oxide yellow paste may lead to flocculation, reducing its stability. This study employed octenyl succinate starch (OSA starch) and Arabic gum (AG) as composite wall materials encapsulate optimize system water‐soluble powders. Transmission electron microscopy (TEM) scanning (SEM) confirmed formation a well‐structured microcapsule, significantly smaller...
Sarcodon aspratus is a popular edible fungus for its tasty flavour and can be used as dietary supplement functional substances. This study was conducted to evaluate the potential health benefits of polysaccharides (SAFP) on water immersion restraint stress (WIRS)-induced gastric ulcer in rats. The results indicated that SAFP could decrease myeloperoxidase (MPO) activity plasma corticosterone levels, well enhance Prostaglandin E2 (PGE2) Nitrate/nitrite (NOx) concentration Furthermore,...
Abstract Sertoli cells are essential for regulating normal spermatogenesis. However, the mechanisms underlying human cell development remain largely elusive. Here we examined function and signaling pathways of BMP6 in cells. RT-PCR, immunocytochemistry Western blots revealed that its multiple receptors were expressed CCK-8 EDU assays showed promoted proliferation Conversely, siRNAs inhibited division these Annexin V/PI assay indicated reduced apoptosis cells, whereas knockdown assumed...
// Zheng Chen 1 , Min Sun Qingqing Yuan Minghui Niu Chencheng Yao Jingmei Hou Hong Wang Liping Wen Yun Liu Li 2 Zuping He 1, 2, 3, 4 State Key Laboratory of Oncogenes and Related Genes, Renji- Med X Clinical Stem Cell Research Center, Ren Ji Hospital, School Medicine, Shanghai Jiao Tong University, 200127, China Department Urology, Institute Andrology, 200001, 3 Assisted Reproduction Reproductive Genetics, 200025, Correspondence to: He, e-mail: zupinghe@sjtu.edu.cn Keywords: human...
The aim of this study is to investigate the implication Chinese herbal formula (CHF) Shenzhu tiaopi Granule (STG) in type 2 diabetes mellitus (T2DM) and discuss mechanisms by which STG regulates gut microbiota.Goto-Kakizaki (GK) rats age-matched Wistar (W) were acclimatized for 1 week. GK randomly divided into 3 groups orally gavaged with saline (model group, M), acarbose (acarbose A), (granule CHF G; component includes Codonopsis pilosula, Rhizoma Atractylodis, Pinellia, Poria cocos,...
Pentagalloylglucose (PGG), a gallotannin polyphenolic compound, has been found to possess host of beneficial pharmacologic activities, such as anti-inflammatory and antioxidative activities. We previously demonstrated that PGG is capable binding the cell membrane renal mesangial cells, but pharmacological effect on diabetic injury underlying mechanisms are still not yet clear. In this study, effects Nrf2/HO-1 JAK2/STAT3 signaling were explored in AGE-stimulated cells. Furthermore, Nrf2...
<b><i>Introduction:</i></b> Membrane-associated guanylate kinase with an inverted domain structure-1 (MAGI1) is dysregulated in diabetes; however, its role diabetic nephropathy (DN) remains unclear. In this study, we determined the function and associated mechanisms of MAGI1 DN. <b><i>Methods:</i></b> Serum samples from 28 patients DN normal volunteers were collected. High-glucose (HG)-treated human renal mesangial cells (HRMCs)...
Previous studies have identified the beneficial effects of electroacupuncture (EA) on motor behaviors in Parkinson's disease (PD). However, role and potential mechanisms EA PD-associated depression remain unclear. In present study, a rat model PD with unilateral 6-hydroxydopamine (6-OHDA) lesions medial forebrain bundle was treated using for 4 weeks. We found that 100 Hz improved several phenotypes. addition, tyrosine hydroxylase (TH) immunohistochemical analysis showed had minimal impact...
Danzhi Jiangtang Capsule (DJC), a traditional Chinese medicinal formula, has been used clinically in treating diabetes and diabetic nephropathy (DN). We previously demonstrated that DJC is capable of improving renal function patients rats with DN, but the mechanisms underlying these therapeutic benefits are not quite clear yet. In this study, STZ-induced were orally administered for 8 weeks. Fasting blood glucose, indicators serum, index, expression proteins related to JAK-STAT signaling...
Clinical and research evidence has shown that electroacupuncture (EA) promotes recovery of motor function in patients with Parkinson's disease (PD). However, the "efficacy span" EA treatment, especially long-term effect is thought to last after cessation not been investigated. The present study thus investigated compared during chronic application on activity dopamine lesions a 6-hydroxydopamine (6-OHDA)-lesioned mouse model PD. Chronic treatment (30 min day, 6 days week for 2 or 4 weeks)...
// Zheng Chen 1, 6, * , Minghui Niu Min Sun Qingqing Yuan Chencheng Yao 1 Jingmei Hou Hong Wang Liping Wen Hongyong Fu Fan Zhou Li 2 Zuping He 3, 4, 5 State Key Laboratory of Oncogenes and Related Genes, Renji- Med X Clinical Stem Cell Research Center, Ren Ji Hospital, School Medicine, Shanghai Jiao Tong University, 200127, China Department Andrology, Urologic Medical General 200080, 3 Institute 200001, 4 Assisted Reproduction Reproductive Genetics, 200025, 6 Surgery, Suqian people's The...
The antidiabetic properties and anti‐inflammatory effects of Danzhi Jiangtang Capsules (DJC) have been demonstrated in clinical laboratory experiments. In this study, we explored whether DJC can ameliorate advanced glycation end products‐ (AGEs‐) mediated cell injury the precise mechanisms treating diabetic nephropathy (DN). Western blot analysis was employed to assess expressions iNOS, COX2, SOCS phosphorylation JAK2, STAT1, STAT3 glomerular mesangial cells (GMCs) after treatment with DJC....
Banxia Xiexin Decoction (BXXD) reportedly regulates glycolipid metabolism and inhibits pancreatic β-cell apoptosis. This study is aimed at investigating the protective effect of BXXD on tert-butyl hydroperoxide- (t-BHP-) induced apoptosis in MIN6 cells underlying mechanisms. were preincubated with or liraglutide (Li) without PI3K inhibitor LY294002 (LY) for 12 h, following which t-BHP was added to induce cell The effects evaluated by detecting viability proliferation glucose-stimulated...
Objective . To analyse the clinical features of diabetic peripheral neuropathy (DPN) and employ data mining technology to explore rules Chinese herbal medicine (CHM) therapy. Methods The 216 patients with DPN qi‐yin deficiency syndrome were obtained, assessed by cluster analysis. Relevant information was entered into diagnosis treatment collection system, techniques used drug frequency, core CHM, CHM pair, so on. Results In this study, glycated haemoglobin (HbA1c) homocysteine (HCY) closely...
In this study, lycopene was successfully encapsulated in polyelectrolyte complex nanoparticles (PEC NPs) fabricated with a negatively charged polysaccharide, TLH-3, and positively sodium caseinate (SC) via electrostatic interactions. Results showed that the lycopene-loaded PEC NPs were spherical shape, have particle size of 241 nm, zeta potential −23.6 mV, encapsulation efficiency 93.6%. Thus, could serve as effective carriers which affected physicochemical characteristics improved its water...