A5088724322- Cardiac Ischemia and Reperfusion
- Cardiac electrophysiology and arrhythmias
- Cardiac Fibrosis and Remodeling
- Ion channel regulation and function
- Nitric Oxide and Endothelin Effects
- Receptor Mechanisms and Signaling
- Bone health and treatments
- Adipose Tissue and Metabolism
- Protein Kinase Regulation and GTPase Signaling
- Cardiovascular Function and Risk Factors
- Cardiomyopathy and Myosin Studies
- Mitochondrial Function and Pathology
- Hormonal Regulation and Hypertension
- Signaling Pathways in Disease
- Pulmonary Hypertension Research and Treatments
- Cardiac Arrest and Resuscitation
- Renin-Angiotensin System Studies
- Cardiovascular Effects of Exercise
- Anesthesia and Neurotoxicity Research
- Lipoproteins and Cardiovascular Health
- Cardiovascular and exercise physiology
- Tissue Engineering and Regenerative Medicine
- Congenital heart defects research
- Fibroblast Growth Factor Research
- Metabolism, Diabetes, and Cancer
Justus-Liebig-Universität Gießen
2015-2024
Institut für Klinische Forschung und Entwicklung
2021
Virtuelle Fabrik (Switzerland)
2021
Siemens (Germany)
2021
Software (Spain)
2021
Behörde für Gesundheit und Verbraucherschutz
2021
Endress Hauser Messtechnik (Germany)
2021
Praxis
2021
University of Oxford
2020
Immanuel Krankenhaus
2020
Transforming growth factor-β 1 (TGF-β ) promotes or inhibits cell proliferation and induces fibrotic processes extracellular matrix production in numerous types. Several cardiac diseases are associated with an increased expression of TGF-β mRNA, particularly during the transition from stable hypertrophy to heart failure. In vitro studies suggest a link between signaling β-adrenergic system. However, vivo effects this factor on myocardial tissue have been poorly identified. transgenic mice...
Alterations of the nitric oxide receptor, soluble guanylate cyclase (sGC) may contribute to pathophysiology pulmonary arterial hypertension (PAH). In present study, expression sGC in explanted lung tissue PAH patients was studied and effects stimulator BAY 63-2521 on enzyme activity, haemodynamics vascular remodelling were investigated two independent animal models PAH. Strong upregulation vessels idiopathic lungs compared with healthy donor demonstrated by immunohistochemistry. Upregulation...
Trophic effects of isoproterenol (Iso), norepinephrine (NE), phenylephrine (PE), and biologically active fragments parathyroid hormone (PTH), PTH-(1–34) PTH-(28–48), were investigated in mechanically quiescent, isolated ventricular cardiomyocytes from adult rat. In 24-h incubations modified serum-free medium 199 incorporation [14C]phenylalanine, changes total protein specific activities cytosolic enzymes, creatine kinase (CK) lactate dehydrogenase (LDH) monitored. NE PE (10 microM), but not...
Although incidence and prevalence of prediabetes are increasing, little is known about its cardiac effects. Therefore, our aim was to investigate the effect on function characterize parameters pathways associated with deteriorated performance. Long-Evans rats were fed either control or high-fat chow for 21 wk treated a single low dose (20 mg/kg) streptozotocin at week 4 High-fat treatment induced as characterized by slightly elevated fasting blood glucose, impaired glucose insulin tolerance,...
Abstract The stimulation of DNA synthesis in primary cell cultures chicken chondrocytes by parathyroid hormone was studied assaying [3H]thymidine incorporation into DNA. Optimal assay conditions were determined varying age, plating density, and incubation time. Under these significantly stimulated (PTH) some its fragments: cells treated with human (h)PTH(1-84), bovine (b)PTH(1-34) [Nle8,18,Tyr34]bPTH(3-34)amide hPTH(13-34) displayed 2.6-fold enhanced a dose-dependent manner. fragment...
Uncontrolled release of Ca2+ from the sarcoplasmic reticulum (SR) contributes to reperfusion-induced cardiomyocyte injury, e.g. hypercontracture and necrosis. To find out underlying cellular mechanisms this phenomenon, we investigated whether opening mitochondrial permeability transition pores (MPTP), resulting in ATP depletion reactive oxygen species (ROS) formation, may be involved. For purpose, isolated cardiac myocytes adult rats were subjected simulated ischemia reperfusion. MPTP was...
Background— Fibroblast growth factor 9 (FGF9) is secreted from bone marrow cells, which have been shown to improve systolic function after myocardial infarction (MI) in a clinical trial. FGF9 promotes cardiac vascularization during embryonic development but only weakly expressed the adult heart. Methods and Results— We used tetracycline-responsive binary transgene system based on α-myosin heavy chain promoter test whether conditional expression of myocardium supports adaptation MI. In...
Recent studies have documented that oxidized low-density lipoprotein cholesterol (oxLDL) levels directly impact myocardial structure and function. However, the molecular mechanisms by which oxLDL affects cardiac myocytes are not well established. We addressed question whether modifies load-free cell shortening, a standardized readout of cellular function, investigated proprotein convertase subtilisin/kexin-9 (PCSK9) is involved on oxLDL-dependent processes. Adult rat ventricular...
Arterial hypertension is the most important risk factor for development of cardiovascular disease. Recently, aircraft noise has been shown to be associated with elevated blood pressure, endothelial dysfunction, and oxidative stress. Here, we investigated potential exacerbated effects in combination experimental arterial hypertension. C57BL/6J mice were infused 0.5 mg/kg/d angiotensin II 7 days, exposed days at a maximum sound pressure level 85 dB(A) mean 72 dB(A), or subjected both...
Myocardial connexin 43 (Cx43) forms gap junctions and hemichannels, is also present within subsarcolemmal mitochondria. The protein phosphorylated by several kinases including mitogen-activated kinase (MAPK), C (PKC), casein 1 (CK1). A reduction in Cx43 content abrogates myocardial infarct size ischemic preconditioning (IPC). study characterizes the contribution of phosphorylation towards mitochondrial function, hemichannel activity, cardioprotection IPC wild-type (WT) mice which...
Adult ventricular cardiomyocytes have been identified as target cells for parathyroid hormone (PTH) but little is known about its signal transduction in these cells. In the present study influence of PTH on cyclic AMP accumulation and activity protein kinase C (PKC) was evaluated. A mid-regional synthetic fragment PTH, PTH-(28-48), which exerts a hypertrophic effect cardiomyocytes, increased membrane-associated PKC dose-dependent manner (1-100 nM). Activated membranous dependent Ca2+...
Hypoxia-reoxygenation induces loss of endothelial barrier function and oedema formation, which presents a major impediment for recovery the organ. The integrity is highly dependent on its contractile machinery actin dynamics, are precisely regulated by Rho GTPases. Perturbed activities these Rho-GTPases under hypoxia-reoxygenation lead to derangement cytoskeleton therefore may affect barrier. aim present study was analyse role GTPases in regulating during cultured porcine aortic cells...
It was investigated whether neuropeptide Y (NPY) could exert a trophic effect on ventricular myocytes isolated from the adult rat heart. Two different culture models were used: day 1 and 7 cultures of cardiomyocytes. In cultures, NPY caused an increase in cellular protein mass. (10 nM) increased protein-to-DNA ratio within 24 h by 10.1 +/- 2.8% (P < 0.01), but did not stimulate incorporation [14C]phenylalanine into cell proteins. The degradation proteins retarded presence NPY, revealed...
Reperfusion injury of the myocardium is characterised by development cardiomyocyte hypercontracture. Previous studies have shown that cGMP-mediated stimuli protect against reperfusion injury, but cellular mechanism still unknown.To simulate ischemia/reperfusion, adult rat cardiomyocytes were incubated anoxically (pH(o) 6.4) and then reoxygenated 7.4). Cytosolic calcium [Ca(2+)](i) (fura-2 ratio), pH(i) (BCECF cell length, phospholamban phosphorylation analysed. Under simulated ischemia...