A5047122522- MicroRNA in disease regulation
- Circular RNAs in diseases
- Respiratory viral infections research
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Intracranial Aneurysms: Treatment and Complications
- Viral gastroenteritis research and epidemiology
- Cancer Research and Treatments
- Pneumonia and Respiratory Infections
- Biochemical effects in animals
- Neurological Disease Mechanisms and Treatments
- Sirtuins and Resveratrol in Medicine
- Bone Metabolism and Diseases
- Nutritional Studies and Diet
- Antioxidant Activity and Oxidative Stress
- Cerebrovascular and Carotid Artery Diseases
- Cardiovascular Health and Disease Prevention
- Barrier Structure and Function Studies
- Cardiovascular Health and Risk Factors
- Traditional Chinese Medicine Analysis
- Traumatic Brain Injury and Neurovascular Disturbances
- Intracerebral and Subarachnoid Hemorrhage Research
The First People's Hospital of Wenling
2013-2019
Wenzhou Medical University
2009-2018
Growing evidence shows that long noncoding RNAs (lncRNAs) play a crucial role in cancer progression. However, whether lncRNA CDKN2BAS is involved human hepatocellular carcinoma (HCC) metastasis remains unclear.Human microarray analysis was performed to detect differential expression levels of lncRNAs metastatic HCC tissues. Effects on cell proliferation, migration, and apoptosis were determined by MTT assay, colony formation migration scratch flow cytometry. The xenograft experiment used...
// Lingfang Teng 1 , Yu Chen Huihui Xijun He Junyou Wang Yujiang Peng Hongyu Duan Huiyong Li Da Lin and Bo Shao Department of Neurosurgery, The First People's Hospital Wenling, Wenling 317500, Zhejiang Province, China Correspondence to: Shao, email: boshaofire@163.com Keywords: circular RNAs (circRNAs); intracranial aneurysms (IA); disease-free survival (DFS); overall (OS); diagnosis Received: July 25, 2017 Accepted: August 28, Published: November 10, ABSTRACT Circular (circRNAs) in the...
Glioblastomas (GBMs) are a lethal class of brain cancer, with median survival <15 months in spite therapeutic advances. The poor prognosis GBM is largely attributed to acquired chemotherapy resistance, and new strategies urgently needed target resistant glioma cells. Here we report role for miR-299-5p GBM. level expression was detected specimens cell lines by qRT-PCR. Luciferase reporter assays Western blots were performed verify GOLPH3 as direct miR-299-5p. In vitro proliferation,...
Carnosic acid (CA) has been reported to exhibit a variety of bioactivities including antioxidation, neuroprotection, and anti-inflammation; however, the impact CA on subarachnoid hemorrhage (SAH) never elucidated. The current study was undertaken explore role in early brain injury (EBI) secondary SAH underlying mechanisms. Adult male Sprague-Dawley rats were perforated mimic clinical aneurysm with SAH. or vehicle administered intravenously immediately after occurred. Mortality, grade,...
Glioblastoma (GBM) is the most common and lethal of intracranial tumors, which characterized by extensive proliferation diffused invasion tumor cells. MicroRNA-193a-5p (miR-193a-5p) have been demonstrated previously as a functional suppressor in development progression various cancers. The current study aimed to investigate whether miR-193a-5p influences cell migration through mitogen-activated protein kinase/extracellular signal-regulated kinase signaling pathway targeting neuro-oncological...
Objective Few comprehensive studies have searched for viruses in infants and young children with community-acquired pneumonia (CAP) China. The aim of this study was to investigate the roles human herpes (HHVs) other respiratory CAP not caused by typical bacterial infection determine their prevalence clinical significance. Methods Induced sputum (IS) samples were collected from 354 hospitalised patients (infants, n = 205; children, 149) illness (CAP or non-CAP) admitted Wenling Hospital We...
Abstract The cardiovascular health (CVH) metrics are closely related to the risk of stroke. Extracranial carotid artery stenosis (ECAS) represents an important factor for ischemic present study aims explore longitudinal effect baseline CVH on development ECAS. Totally 5,440 participants were randomly enrolled in Asymptomatic Polyvascular Abnormalities Community from 2010 2011. Information regarding seven was collected at baseline. ECAS assessed by performing duplex sonography (2010–2011) and...
Objective To investigate pave a way for studying pathogenicty of HBoV.Methods Isolation and cell culture HBoV by human bronchial epithelial line.which was founded in our laboratory.The morphology the virus were primarily studied with transmission electron microscope.In addition,transcript mRNA detected cells,which passaged infected within HBoV,using reverse-transcription polymerase chain reaction(RT-PCR).The amplified products nucleotide sequence sequencing analysis.Results Cytopathic...
Objective To investigate the expression of glial fibrillary acidic protein (GFAP),Beclin-1 and microtubule-associated 2 (MAP2) after subarachnoid hemorrhage (SAH) in rats,and explore relationship between early brain injury autophagy process SAH.Methods A total 40 adult male SD rats were randomly divided into sham group SAH 6 h,24 h 3 day groups.Rats decapitated under anesthesia induction.The brains immediately removed separated hemispheres cerebellums.The water content each sample was...
Objective To clone and express VP, gene from HBoV, the expressed protein was as antigen in order to detect serum children Wenling area with lower respiratory tract infections. Methods The recombined genome of Baculovirus, which infected insect cell. fusion HA tag applied confirm specificity protein. Furthermore, recombinant observed using electron microscopy. 176 infections screened Western blot. Results VP2 more than 60% total proteins cell, MWt about 60 x 10(3). virus-like particle (VLP)...