A5041873395- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Cancer, Lipids, and Metabolism
- Cancer Cells and Metastasis
- Phagocytosis and Immune Regulation
- Prostate Cancer Treatment and Research
- Wnt/β-catenin signaling in development and cancer
- Pancreatic and Hepatic Oncology Research
- Monoclonal and Polyclonal Antibodies Research
- Bladder and Urothelial Cancer Treatments
- Lung Cancer Treatments and Mutations
- Circular RNAs in diseases
- Galectins and Cancer Biology
- Pancreatic function and diabetes
- Inflammatory mediators and NSAID effects
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
Vanderbilt University
2018-2024
Vanderbilt University Medical Center
2019-2024
Florida State University
2015-2016
Abstract Small extracellular vesicles called exosomes affect multiple autocrine and paracrine cellular phenotypes. Understanding the function of requires a variety tools, including live imaging. Our previous live-cell reporter, pHluorin-CD63, allows dynamic subcellular monitoring exosome secretion in migrating spreading cells. However, dim fluorescence inability to make stably-expressing cell lines limit its use. We incorporated stabilizing mutation pHluorin moiety, M153R, which now exhibits...
Mammalian cells release a heterogeneous array of extracellular vesicles (EVs) that contribute to intercellular communication by means the cargo they carry. To resolve EV heterogeneity and determine if is partitioned into select populations, we developed method named "EV Fingerprinting" discerns distinct vesicle populations using dimensional reduction multiparametric data collected quantitative single-EV flow cytometry. were found be discernible combination membrane order size, both which...
Protein profiling studies of prostate cancer have been widely used to characterize molecular differences between diseased and non-diseased tissues. When combined with pathway analysis, approaches are able identify mechanisms cancer, group patients by subtype, predict prognosis. This strategy can also be implemented study in very specific populations, such as African Americans who higher rates incidence mortality than other racial groups the United States. In this study, age-, stage-, Gleason...
Genomic technologies including microarrays and next-generation sequencing have enabled the generation of molecular signatures prostate cancer. Lists differentially expressed genes between malignant non-malignant states are thought to be fertile sources putative cancer biomarkers. However such lists can highly variable for multiple reasons. As such, looking at differential expression in context gene sets pathways has been more robust. Using genome data from The Cancer Genome Atlas, age-...
Abstract Extracellular vesicles (EVs) are small, lipid‐bilayer‐bound particles released by cells that can contain important bioactive molecules, including lipids, RNAs, and proteins. Once in the extracellular environment, EVs act as messengers locally well to distant tissues coordinate tissue homeostasis systemic responses. There is a growing interest not only understanding physiology of signaling but also leveraging them minimally invasive diagnostic prognostic biomarkers (e.g., they be...
Esophageal adenocarcinoma (EAC) is a highly aggressive malignancy that characterized by resistance to chemotherapy and poor clinical outcome. The overexpression of the receptor tyrosine kinase AXL frequently associated with unfavorable prognosis in EAC. Although it well documented mediates cancer cell invasion as downstream effector epithelial-to-mesenchymal transition, precise molecular mechanism underlying this process not completely understood. Herein, we demonstrate for first time...
While many adhesion receptors are known to influence tumor progression, the mechanisms by which they dynamically regulate cell-cell remain elusive. We previously identified Activated Leukocyte Cell Adhesion Molecule (ALCAM) as a clinically relevant driver of metastasis and hypothesized that tunable mechanism ectodomain shedding regulates its contribution dissemination. To test this hypothesis, we examined an under-explored ALCAM splice variant (ALCAM-Iso2) demonstrated loss membrane-proximal...
Small extracellular vesicles called exosomes affect a variety of autocrine and paracrine cellular phenotypes, including migration, immune activation, neuronal function. Understanding the function requires tools, live cell imaging. We previously constructed live-cell reporter, pHluorin-CD63, that allows dynamic subcellular monitoring exosome secretion in migrating spreading cells. However, there were some caveats to its use, dim fluorescence inability make lines stably express protein. By...
Filopodia are dynamic adhesive cytoskeletal structures that critical for directional sensing, polarization, cell-cell adhesion, and migration of diverse cell types. also neuronal synapse formation. While rearrangement the actin cytoskeleton is known to be filopodia biogenesis, little about upstream extracellular signals. Here, we identify secreted exosomes as potent regulators Inhibition exosome secretion inhibited formation stabilization in both cancer cells neurons subsequent by neurons....
Filopodia are dynamic adhesive cytoskeletal structures that critical for directional sensing, polarization, cell-cell adhesion, and migration of diverse cell types. also neuronal synapse formation. While rearrangement the actin cytoskeleton is known to be filopodia biogenesis, little about upstream extracellular signals. Here, we identify secreted exosomes as potent regulators Inhibition exosome secretion inhibited formation stabilization in both cancer cells neurons subsequent by neurons....
Filopodia are dynamic adhesive cytoskeletal structures that critical for directional sensing, polarization, cell-cell adhesion, and migration of diverse cell types. also neuronal synapse formation. While rearrangement the actin cytoskeleton is known to be filopodia biogenesis, little about upstream extracellular signals. Here, we identify secreted exosomes as potent regulators Inhibition exosome secretion inhibited formation stabilization in both cancer cells neurons subsequent by neurons....
Abstract Mammalian cells release a heterogeneous array of extracellular vesicles (EVs) that impact human biology by contributing to intercellular communication. To resolve EV heterogeneity and define the populations associated with specific biological processes, we developed method named “EV Fingerprinting” discerns distinct vesicle using dimensional reduction multi-parametric data collected quantitative single-EV flow cytometry. After validating this against synthetic standards,...