A5027517225- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- Peptidase Inhibition and Analysis
- Chemical Synthesis and Analysis
- Biotin and Related Studies
- Advanced biosensing and bioanalysis techniques
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Acute Lymphoblastic Leukemia research
- Immunotherapy and Immune Responses
- Carbohydrate Chemistry and Synthesis
- Innovative Microfluidic and Catalytic Techniques Innovation
- Lymphoma Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- Biochemical and Structural Characterization
- Neuroendocrine Tumor Research Advances
- Radiopharmaceutical Chemistry and Applications
- Cell Adhesion Molecules Research
- Electrochemical sensors and biosensors
ETH Zurich
2016-2025
Philochem (Switzerland)
2021
Universidad de La Rioja
2019
Center for Systems Biology
2016
Significance Fibroblast activation protein (FAP) has recently emerged as a tumor-associated antigen with abundant and selective expression in the majority of human solid malignancies. To best our knowledge, OncoFAP is highest-affinity small organic FAP ligand reported to date, dissociation constant 680 pM, measured by fluorescence polarization. Upon intravenous administration, both fluorescent radiolabeled derivatives exhibited rapid accumulation FAP-positive tumors, sparing normal tissues....
The characterization of peptides bound to human leukocyte antigen (HLA) class I is fundamental importance for understanding CD8+ T cell-driven immunological processes and the development immunomodulatory therapeutic strategies. However, until now, mass spectrometric analysis HLA-bound has typically required billions cells, still resulting in relatively few high-confidence peptide identifications. Capitalizing on recent developments spectrometry bioinformatics, we have implemented a...
The first drugs discovered using DNA-encoded chemical library (DEL) screens have entered late-stage clinical development. However, DEL technology as a whole still suffers from poor purity resulting in suboptimal performance. In this work, we report technique to overcome issue through self-purifying release of the after magnetic bead-based synthesis. Both and last building blocks each assembled member were linked beads by tethers that could be cleaved mutually orthogonal chemistry. Sequential...
T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematological cancer for which treatment options often result in incomplete therapeutic efficacy and long-term side-effects. Interleukin 7 (IL-7) its receptor IL-7Rα promote T-ALL development mutational activation of associates with very high risk relapsed disease. Using combinatorial phage-display libraries antibody reformatting, we generated a fully human IgG1 monoclonal (named B12) against both wild-type mutant IL-7Rα, predicted...
Abstract Cyclic peptides constitute an important drug modality since they offer significant advantages over small molecules and macromolecules. However, access to diverse chemical sets of cyclic is difficult on a large library scale. DNA-encoded Chemical Libraries (DELs) provide suitable tool obtain diversity, but DELs made by standard DEL implementation cannot efficiently explore their conformational diversity. On the other hand, dual-display Encoded Self-Assembling (ESAC) can be used for...
Soluble human leukocyte antigen class I (sHLA)-peptide complexes have been suggested to play a role in the modulation of immune responses and evasion cancer cells. The set peptides eluted from sHLA molecules could serve as biomarker for monitoring patients with or other conditions. Here, we describe an improved peptidomics methodology resulting identification 1816 2761 unique peptide sequences triplicate analyses serum plasma taken three healthy donors. More than 90% identified were 8-11mers...
Salicylaldehyde (SA) derivatives are emerging as useful fragments to obtain reversible-covalent inhibitors interacting with the lysine residues of target protein. Here SA installation at C terminus an integrin-binding cyclopeptide, leading enhanced ligand affinity for receptor well stronger biological activity in cultured glioblastoma cells is reported.