Tracheal implants using tissue-engineering and three-dimensional (3D) printing are promising, but challenges remain, including graft composition, anastomosis methods, tracheal tissue regeneration. This study examines the effectiveness of various materials in a large animal model. Thirty-eight surgeries were performed on porcine model, involving 2 cm circumferential excision with end-to-end anastomosis. The grafts used included translucent plastic material crystal (n=16), silicone (n=8),...

The use of a nonthermal plasma (NTP) jet in the treatment living tissue has been subject considerable interest field medical technology, and potential to reduce recovery time open wounds. We aimed investigate wound-healing process by clinical observation, blood tests, expression cell adhesion markers reactive oxygen species NTP jet-treated rats.This study utilized Sprague-Dawley (SD) rats as experimental subjects, wounds measuring 2 cm × were produced on animals' backs. group was treated...

Tracheal cartilage has limited regenerative potential in adults, posing a significant challenge for tracheal repair. Here, we observed marked neocartilage growth porcine model after transplanting it with three-dimensional (3D)-printed silicone graft. Virtual grafts of 2 cm length were first generated using 3D printer, and simulated 3-month-old trachea based on data from physical stress tests. After segmental resection, graft underwent end-to-end anastomosis at both the proximal distal...

Background/Aim: Metalloproteinases (MMPs) are a family of multifunctional proteins reported to be overexpressed in several types cancers. However, the contribution MMP8 genotype oral cancer has not been elucidated. In this study, we focused on polymorphisms promoter region MMP-8 (C-799T) and two non-synonymous (Val436Ala Lys460Thr) Taiwanese cancer. Materials Methods: case-control genotype, was examined among 788 patients with 956 gender- age-matched healthy controls regarding its potential...

Diabetic retinopathy is characterized by vasopermeability, vascular leakage, inflammation, blood-retinal barrier breakdown, capillary degeneration, and neovascularization. However, the mechanisms underlying association between diabetes mellitus progression remain unclear.TPL2 (tumor locus 2), a serine-threonine protein kinase, exerts pathological effect on angiogenesis. This study investigated role of Nε-(carboxymethyl)lysine, major advanced glycation end products, involved TPL2-related...

Matrix metalloproteinases (MMPs) play important roles in inflammation and carcinogenesis, but the genotypic role of MMP-7 has never been investigated colorectal cancer (CRC) among Taiwanese. Therefore, this study we aimed to evaluate contribution genotypes risk CRC Taiwan.In case-control study, A-181G C-153T promoter were determined their association with 362 patients age- gender-matched healthy controls. In addition, interaction personal behaviors also examined.The percentages variant AG GG...

Polycaprolactone (PCL) implants in large animals show great promise for tracheal transplantation. However, the longest survival time achieved to date is only about three weeks. To meet clinical application standards, it essential extend and ensure complete integration functionality of implant. Our study investigates use three-dimensional (3D)-printed, biodegradable, PCL-based grafts large-scale porcine transplantation, assessing feasibility early structural integrity crucial long-term...

Matrix metalloproteinases (MMPs) play a critical role in inflammation and carcinogenesis, the expression of mRNA MMP7 oral squamous cell carcinoma tissues was higher than lichen planus or normal mucosa. However, genotypic has never been examined cancer. Therefore, current study we aimed to examine contribution variants promoter region (A-181G C-153T) cancer risk Taiwan.In this hospital-based case-control study, 788 patients with 956 gender-and age-matched healthy controls were genotyped for...

Bladder cancer is the sixth most common worldwide and its incidence particularly high in many developed regions including southwestern Taiwan. However, genetic contribution to etiology of bladder not well-understood. The aim this study was evaluate association enhancer zeste homolog 2 (EZH2) genotypes with Taiwan risk.Three polymorphic variants EZH2 were analyzed regarding their risk, three hundred seventy-five patients same number age- gender-matched healthy controls recruited genotyped by...

Background. Acute tubular necrosis (ATN) is characterized by an initiation phase, followed extension and a maintenance recovery the latter of which involves increased regeneration cells. Nephronectin (NPNT), ligand for α8β1 integrin, expressed in ureteric bud epithelium during kidney morphogenesis. However, little known about potential involvement NPNT phase ATN.

Background/Aim: Accumulated evidence has supported the notion that matrix metalloproteinase (MMP) genotypes are associated with susceptibility of many types cancers. However, few reports have studied contribution MMP to either diagnostic or prognostic potential in non-solid tumors such as leukemia. In this study, we firstly investigated a polymorphism promoter region MMP-8 (-799C/T) and two non-synonymous polymorphisms (Val436Ala Lys460Thr) childhood Patients Methods: 266 patients acute...

The genomic role of human mouse double minute 2 (MDM2) in colorectal cancer (CRC) is unclear, therefore, our study aimed to evaluate the contribution MDM2 genotype risk CRC Taiwan.In this case-control study, SNP309 T G (rs2279744) genotypes were determined and their association with investigated among 362 patients age- gender-matched healthy controls central Taiwan. In addition, interaction personal behaviors clinicopathological features also examined.The percentage variant GG for was 30.9%...

Metalloproteinase 2 (MMP2) is a multi-functional protein which has been shown to be up-regulated in patients with oral cancer, especially those lymph node metastasis. However, the association of MMP2 genotype cancer risk or metastatic behavior unknown. This study aimed evaluate role promoter 1306 and -735 genotypes metastasis.In this case-control study, (rs243865) (rs2285053) their interaction consumption areca, cigarettes, alcohol determining were investigated among 788 956 gender-matched...

Trypanosoma (subgenus Megatrypanum) theileri was first identified over one hundred years ago, and is a widespread parasite in cattle. Its life cycle within the mammalian host has rarely been reported. Whether there an intracellular stage tissues unknown such not demonstrated experimentally. Intriguingly, using Giemsa staining with light microscopy transmission electron examination, we found that able only to attach cells but also invade several phagocytic non-phagocytic cells. Based on these...

Background: This study aimed to investigate the relationship between oxidized regenerated cellulose (ORC) and mucosa healing in an experimental animal model. Methods: Fifteen adult Sprague-Dawley rats were randomly divided into three groups that underwent different wound treatments. In Group 1, no pharyngeal was created. 2, sutured with Prolene only. 3, Prolene, covered one layer of ORC before closure skin wound. The animals euthanized either 5 or 10 days after operation, conditions...

Background/Aim: Pterygium is composed of proliferating fibrovascular tissue, and its formation progression are closely related to the homeostasis extracellular microenvironment. However, few studies have examined contribution matrix metalloproteinases (MMP) either diagnostic or prognostic potential in pterygium. In this study, we investigated a polymorphism promoter region MMP-8 (-799C/T) two non-synonymous polymorphisms (Val436Ala Lys460Thr) Materials Methods: 134 patients with pterygium...

Aim: Arsenic trioxide (As₂O₃), known as pi-shuang and the most toxic compound in traditional Chinese medicine, has been used an antitumor agent for thousands of years. Resveratrol (3,5,4'-trihydroxy-trans-stilbene) is a natural phenol that significant anti-bacterial, anti-fungaI antiaging activities. Our study aimed to examine combined anticancer effects As₂O₃ resveratrol against human neuroblastoma SK-N-SH cells, elucidate underlying intracellular...

The change of optic disc can be used to diagnose many eye diseases, such as glaucoma, diabetic retinopathy and macular degeneration. Moreover, retinal blood vessel pattern is unique for human beings even identical twins. It a highly stable in biometric identification. Since the beginning nerve main vessels retina, it reference point Therefore, segmentation an important technique developing identity recognition system disease diagnostic system. This article hence presents method extract from...

Background/Aim: The aim of this study was to examine the role caspase-8 rs3834129 polymorphism on colorectal cancer (CRC) risk in Taiwanese CRC patients and healthy controls. Materials Methods: (-652 6N insertion/deletion) polymorphic genotypes were analyzed 362 with same number age- gender-matched subjects. interaction personal behaviors clinicopathological features also examined. Results: percentage variants ID DD for genotype 37.6 5.8% group 39.0 6.6% control group, respectively (p...

In this study, we investigated the effects of treating dental implants made from titanium with argon based non-thermal plasma, immediately before insertion on implant stability and bone formation. Biodegradable sandblasting acid etching had been previously used to modify surface implants.To obtain data for 4-time points in triplicate references, a total 36 were divided into 2 groups; 18 served as experimental group received spray containing while other controls. Two treated two untreated...

(1) Background: This study investigates the effects of Ursodeoxycholic acid (UDCA) on NF-κB signaling, farnesoid X receptor (FXR) singling, and microRNA-21 in HepG2 cells. (2) Methods: cells were treated with lipopolysaccharide (LPS) to simulate hepatic inflammation. The investigation focused expression activation, which was analyzed using Western blot, confocal microscopy, Electrophoretic Mobility-shift Assays (EMSA). Additionally, singling expressions micro-RNA-21, COX-2, TNF-α, IL-6,...

Robust neotissue growth including cartilage, muscle, adipose and glands was observed on the external surface of 3D-printed tracheal grafts transplanted into a 3-month-old large-scale porcine, noticeable after 7 days. This study is first to categorize regeneration stages in detail, aiming for clearer understanding cell development process, as previously studies have not fully elucidated chondrogenesis glandogenesis mechanisms. We’ve identified 4 based chondrocyte numbers, protein...