A5100786466- Hearing, Cochlea, Tinnitus, Genetics
- RNA regulation and disease
- Cellular transport and secretion
- Vestibular and auditory disorders
- RNA and protein synthesis mechanisms
- Pluripotent Stem Cells Research
- Chromatography in Natural Products
- Environmental Toxicology and Ecotoxicology
- RNA Research and Splicing
- Lipid metabolism and biosynthesis
- Parasite Biology and Host Interactions
- Sphingolipid Metabolism and Signaling
- CRISPR and Genetic Engineering
- Aquaculture Nutrition and Growth
- Genomics and Chromatin Dynamics
- GABA and Rice Research
- Circular RNAs in diseases
- Peanut Plant Research Studies
- Advanced biosensing and bioanalysis techniques
- Nuclear Receptors and Signaling
- Wnt/β-catenin signaling in development and cancer
- Lipid Membrane Structure and Behavior
- Cancer-related molecular mechanisms research
- Signaling Pathways in Disease
- Pharmaceutical and Antibiotic Environmental Impacts
Fujian Agriculture and Forestry University
2022-2025
King's College London
2014-2024
University of Kentucky
2023
Zhejiang University
2005-2022
Wellcome Sanger Institute
2014-2022
Northwest A&F University
2022
Nanchang University
2022
Shandong University
2022
Nanjing Agricultural University
2022
Children's Hospital of Fudan University
2022
Adult-onset hearing loss is very common, but we know little about the underlying molecular pathogenesis impeding development of therapies. We took a genetic approach to identify new molecules involved in by screening large cohort newly generated mouse mutants using sensitive electrophysiological test, auditory brainstem response (ABR). review here findings from this screen. Thirty-eight unexpected genes associated with raised thresholds were detected our unbiased sample 1,211 tested,...
Spinster homolog 2 (Spns2) acts as a Sphingosine-1-phosphate (S1P) transporter in zebrafish and mice, regulating heart development lymphocyte trafficking respectively. S1P is biologically active lysophospholipid with multiple roles signalling. The mechanism of action Spns2 still elusive mammals. Here, we report that Spns2-deficient mice rapidly lost auditory sensitivity endocochlear potential (EP) from to 3 weeks old. We found progressive degeneration sensory hair cells the organ Corti, but...
Glutathione peroxidases (GPX) catalyze the reduction of H2O2 or organic hydroperoxides to water corresponding alcohols using reduced glutathione, which plays an essential role in ROS (reactive oxygen species) homeostasis and stress signaling. Thellungiella salsuginea (Eutrema salsugineum), a relative Arabidopsis thaliana, displays extremely high level tolerance salt, drought, cold oxidative stresses. The enzymatic antioxidant systems may contribute T. salsuginea. In present study, we aimed...
There is growing evidence that Bit1 exerts different roles in the development and progression of human cancers. Although was highly exhibited ESCC tissues our previous study, its molecular mechanisms implicated remain unknown. protein expression cell lines normal esophageal epithelial detected by Western blotting. mediated shRNA investigated MTT, migration assay, invasion experiment, ELISA Flow cytometry were utilized to determine effects knockdown on proliferation, migration, apoptosis,...
It is important to find specific and easily detectable diagnostic markers in acute stage of spinal cord injury for guiding treatment estimating prognosis. Although, microRNAs are attractive biomarkers, there still no uniform standard clinical evaluation based on “free circulation” miRNA spectrum. The reason may be that analysis from biological fluids influenced by many pre-analysis variables. Exosome miRNAs widely distributed body have advantages comparing with free miRNAs. the central...
Abstract Progressive hearing loss is very common in the population but we still know little about underlying pathology. A new spontaneous mouse mutation (stonedeaf, stdf ) leading to recessive, early-onset progressive was detected and exome sequencing revealed a Thr289Arg substitution Sphingosine-1-Phosphate Receptor-2 ( S1pr2 ). Mutants aged 2 weeks had normal sensitivity, at 4 most showed variable degrees of impairment, which became severe or profound all mutants by 14 weeks. Endocochlear...
Sgms1 encodes sphingomyelin synthase 1, an enzyme in the sphingosine-1-phosphate signalling pathway, and was previously reported to underlie hearing impairment mouse. A new mouse allele,
miR-96 is a microRNA, non-coding RNA gene which regulates wide array of downstream genes. The mouse mutant diminuendo exhibits deafness and arrested hair cell functional morphological differentiation. We have previously shown that several genes are markedly downregulated in the organ Corti; one these Ptprq, known to be important for maturation maintenance cells. In order study contribution downregulation Ptprq makes phenotype, we carried out microarrays, scanning electron microscopy single...
Background Fatty acid desaturases are enzymes that introduce double bonds into fatty acyl chains. Extensive studies of have been done in many plants. However, less is known about the diversity this gene family peanut (Arachis hypogaea L.), an important oilseed crop cultivated worldwide. Results In study, twelve novel AhFADs genes were identified and isolated from peanut. Quantitative real-time PCR analysis indicated transcript abundances AhFAB2-2 AhFAD3-1 higher seeds than other tissues...
Progressive hearing loss is very common in the human population but we know little about underlying molecular mechanisms. Synaptojanin2 (Synj2) has been reported to be involved, as a mouse mutation led progressive increase auditory thresholds with age. phosphatidylinositol phosphatase which removes 5-position phosphates from phosphoinositides, such PIP2 and PIP3, key enzyme clathrin-mediated endocytosis. To investigate mechanisms loss, have studied different of Synj2 look for any evidence...
Discovery of deafness genes and elucidating their functions have substantially contributed to our understanding hearing physiology its pathologies. Here we report on DNA variants in MINAR2 , encoding membrane integral NOTCH2-associated receptor 2, four families underlying autosomal recessive nonsyndromic deafness. Neurologic evaluation affected individuals at ages ranging from 4 80 y old does not show additional abnormalities. is a recently annotated gene with limited functional...
The genes Ocm (encoding oncomodulin) and Slc26a5 prestin) are expressed strongly in outer hair cells both involved deafness mice. However, it is not clear if they influence the expression of each other. In this study, we characterise auditory phenotype resulting from two new mouse alleles,
Abstract Background Mice carrying targeted mutations are important for investigating gene function and the role of genes in disease, but off-target mutagenic effects associated with processes generating alleles, instance using Crispr, culturing embryonic stem cells, offer opportunities spontaneous to arise. Identifying relies on detection phenotypes segregating independently having a broad estimate level generated by intensive breeding programmes is difficult given that many easy miss if not...
Abstract Mice carrying targeted mutations are important for investigating gene function and the role of genes in disease, but process culturing embryonic stem cells during making a allele offers opportunities spontaneous to arise. Identifying relies on detection phenotypes segregating independently alleles, many easy miss if not specifically looked for. Here we present data from large, knockout programme which mice were analysed through phenotyping pipeline. Twenty-five lines out 1311...
Abstract Background Compared to the ‘neutral’ E3, E4 allele of Apolipoprotein E (APOE) confers up a 15‐fold increase in Alzheimer’s Disease (AD) risk. Conversely, neuroprotective E2 decreases AD risk by similar degree. APOE’s strong profile and multitude effects make it promising therapeutic target. Here, we aimed assess potential allelic ‘switching’ investigating physiological changes associated with an inducible, vivo APOE4 APOE2 transition novel transgenic mouse model. Method The APOE...
Abstract Desmogleins and desmocollins are desmosomal cadherins that make up the adhesive core of desmosomes, intercellular junctions specialized for helping cells withstand mechanical stress. Desmoglein 2 (Dsg2) is most widely expressed member Dsg subfamily misregulation Dsg2 has been reported in many types cancer. While endocytosis known to be a critical determinant regulating cell surface levels classic during tissue remodeling cancer progression, less about regulation cadherins. The...