A5002197987- Algal biology and biofuel production
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Photosynthetic Processes and Mechanisms
- Lipid metabolism and biosynthesis
- Aquaculture Nutrition and Growth
- Environmental Toxicology and Ecotoxicology
- Antioxidant Activity and Oxidative Stress
- Biofuel production and bioconversion
- Trace Elements in Health
- Bacterial Genetics and Biotechnology
- Protein Hydrolysis and Bioactive Peptides
- Venomous Animal Envenomation and Studies
- Mercury impact and mitigation studies
- Meat and Animal Product Quality
- Advanced Nanomaterials in Catalysis
- Plant biochemistry and biosynthesis
- Infant Nutrition and Health
- Seaweed-derived Bioactive Compounds
- Enzyme Production and Characterization
- Probiotics and Fermented Foods
- 3D Printing in Biomedical Research
- Heavy Metal Exposure and Toxicity
- Garlic and Onion Studies
- Polysaccharides and Plant Cell Walls
Henan Agricultural University
2025
Xiamen University
2015-2024
Fujian Fisheries Research Institute
2021
Biochemical Society
2017
Tianjin University of Commerce
2002
Osaka University
2000
Polyunsaturated fatty acids (PUFAs) have been widely applied in the food and medical industry. In this study, malonyl-CoA: ACP transacylase (MAT) was overexpressed through homologous recombination to improve PUFA production Schizochytrium. The results showed that lipid concentration were increased by 10.1 24.5% with MAT overexpression, respectively. Metabolomics analysis revealed intracellular tricarboxylic acid cycle weakened glucose absorption accelerated engineered strain. mevalonate...
Summary Xanthophyllomyces dendrorhous is a promising source of natural astaxanthin due to its ability accumulate high amounts astaxanthin. This study showed that 6‐benzylaminopurine (6‐BAP) an effective substrate enhances cell biomass and accumulation in X. . In the current study, content were determined be improved by 21.98% 24.20%, respectively, induced 6‐BAP treatments. To further understand metabolic responses 6‐BAP, time‐course metabolomics gene expression levels cultures with without...
Abstract Background The hydrolysis and transphosphatidylation of phospholipase D (PLD) play important roles in the interconversion phospholipids (PLs), which has been shown to profoundly impact lipid metabolism plants. In this study, effect PLD1 gene Schizochytrium limacinum SR21 ( S. SR21) on was investigated. Results knockout had little cell growth production, but it significantly improved percentage polyunsaturated fatty acids lipids, docosahexaenoic acid (DHA) content increased by 13.3%...
L-tryptophan is an essential amino acid widely used in food and pharmaceutical industries. However, its production via Escherichia coli fermentation suffers severely from both low glucose conversion efficiency acetic inhibition, to date effective process control methods have rarely been explored facilitate industrial scale production. To resolve these challenges, the current research engineered strain of E. was overproduce L-tryptophan. achieve this, a novel dynamic strategy which...
The polyketide synthase (PKS) cluster genes are supposed to synthesize polyunsaturated fatty acids (PUFAs) in S. limacinum. In this study, two enyolreductase (ER) located on PKS were knocked out through homologous recombination explore their functions. knock-out of OrfB-ER (located OrfB subunit) decreased lipid content and had obvious decrease PUFAs content, indicating domain played a vital role synthesis; the OrfC-ER OrfC SFAs increased total was likely be related with synthesis, production...
Heavy metals are known to reduce the activities of antioxidant enzymes (e.g. superoxide dismutase, catalase), while organophosphorous insecticides inhibit activity enzyme acetylcholinesterase. In this study, these three in zebrafish (Danio rerio) tissues were assessed evaluate consequences heavy metal and organophosphate contamination aquatic systems. When fish contacted with water containing a single pollutant, dismutase was affected by presence Cd but not methyl parathion or Zn. However,...
Schizochytrium sp. is a marine fungus with great potential as an alternative commercial source of lipids rich in polyunsaturated fatty acids (PUFAs). To further increase lipid accumulation sp., the effect exogenous additives has become one hotspots current research. Although benzoic acid derivatives showed positive effects on Schizochytrium, biochemical mechanism needs investigation.Four (sodium benzoate, p-aminobenzoic acid, p-methyl and folic acid) were screened evaluated for their...
Organic carbon sources have been reported to simultaneously increase the growth and lipid accumulation in microalgae. However, there no studies of mixotrophic Porphyridium purpureum organic media. In this study, three sources, glucose, sodium acetate, glycerol were used as substrates for P. purpureum. Moreover, a novel trait-based approach combined with Generalized Additive Modeling was conducted determine dosage each source that optimized concentration cell biomass or fatty acid. A 0.50%...
ABSTRACT The administration of probiotics is an effective approach for treatment Helicobacter pylori (H. pylori), which associated with human gastrointestinal diseases and cancers. To explore more H. infection elimination, bacteria from infant feces were screened in this study. We successfully isolated the Bifidobacterium animalis subsp. lactis strains evaluated its efficacy to inhibit growth vitro vivo. results showed that a B. strain (named BB18) sustained high survival rate after...
Schizochytrium has been widely used in industry for synthesizing polyunsaturated fatty acids (PUFAs), especially docosahexaenoic acid (DHA). However, unclear biosynthesis pathway of PUFAs inhibits further production the Schizochytrium. Unsaponifiable matter (UM) from mevalonate is crucial to cell growth and intracellular metabolism all higher eukaryotes microalgae. Therefore, regulation UM may have important effects on synthesis. Moreover, it well known that UMs, such as squalene β-carotene,...