A5061918014- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Immunology Research
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Plant Virus Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Respiratory viral infections research
- interferon and immune responses
- Microbial infections and disease research
- Influenza Virus Research Studies
- Herpesvirus Infections and Treatments
- Probiotics and Fermented Foods
- Immune Response and Inflammation
- Aquaculture disease management and microbiota
- Advanced Drug Delivery Systems
- COVID-19 Clinical Research Studies
- RNA modifications and cancer
- Plant Gene Expression Analysis
- Ziziphus Jujuba Studies and Applications
- Hepatitis Viruses Studies and Epidemiology
- Potato Plant Research
- Aerogels and thermal insulation
Jiangsu Academy of Agricultural Sciences
2014-2025
Yangzhou University
2019-2025
Hebei Agricultural University
2022-2025
Jiangsu University
2019-2025
Nanjing Agricultural University
2012-2025
Ministry of Science ICT and Future Planning
2023-2025
Ministère de l'Agriculture et de la Souveraineté alimentaire
2024-2025
Ministry of Agriculture and Rural Affairs
2024-2025
State Key Laboratory of Food Science and Technology
2021-2024
Taizhou University
2024
Abstract With a possible origin from bats, the alphacoronavirus Porcine epidemic diarrhea virus (PEDV) causes significant hazards and widespread epidemics in swine population. However, ecology, evolution, spread of PEDV are still unclear. Here, 149,869 fecal intestinal tissue samples pigs collected an 11-year survey, we identified as most dominant diarrheal animals. Global whole genomic evolutionary analyses 672 strains revealed fast-evolving genotype 2 (G2) main viruses worldwide, which...
Porcine epidemic diarrhea virus (PEDV) continues to harm the global swine industry. It is important develop a highly effective vaccine control PEDV infection. Here, we report spike (S) mRNA that primes potent antibody response and antigen-specific T-cell responses in immunized piglets. Active passive immunization can protect piglets against PED following challenge. This study highlights efficiency of PEDV-S represents viable approach for developing an efficient vaccine.
Abstract Background With the effective prevention and control of COVID‐19 in China, number cured cases has increased significantly. Further monitoring disease prognosis “relapse” epidemic become next focus work. This study analysed clinical discharged patients by their SAR‐CoV‐2 nucleic acid status, which provided a theoretical basis for medical institutions to formulate discharge standards follow‐up management patients. Methods We included 13 who were quarantined 4 weeks at home. The...
Porcine deltacoronavirus (PDCoV) can experimentally infect a variety of animals. Human infection by PDCoV has also been reported. Consistently, use aminopeptidase N (APN) from different host species as receptors to enter cells. To understand this broad receptor usage and interspecies transmission PDCoV, we determined the crystal structures binding domain (RBD) spike protein bound human APN (hAPN) porcine (pAPN), respectively. The two complexes exhibit high similarity. RBD binds common...
ABSTRACT Variant Porcine epidemic diarrhea virus (PEDV), which causes and high mortality in piglets, has become a major pathogen, co-epidemics of different subtypes the have very thorny problem for clinical prevention control PEDV. However, cross-protection between G2a G2b subtype strains not been observed, there is currently no vaccine against both strains. In this study, we demonstrate low with piglet immunization challenge tests. The trimeric full-length S proteins variants were purified...
Cohesin is a multi-subunit nuclear protein complex that coordinates sister chromatid separation during cell division. Highly frequent somatic mutations in genes encoding core cohesin subunits have been reported multiple cancer types. Here, using genome-wide CRISPR-Cas9 screening approach to identify host dependency factors and novel innate immune regulators of rotavirus (RV) infection, we demonstrate the loss STAG2, an important component complex, confers resistance RV replication culture...
Rotaviruses (RVs), a leading cause of severe diarrhea in young children and many mammalian species, have evolved multiple strategies to counteract the host innate immunity, specifically interferon (IFN) signaling through RV non-structural protein 1 (NSP1). However, whether structural components also subvert antiviral response remains under-studied. Here, we found that MAVS, critical for RNA sensing pathway upstream IFN induction, is degraded by methyl- guanylyl-transferase (VP3)...
Swine coronavirus-porcine epidemic diarrhea virus (PEDV) with specific susceptibility to pigs has existed for decades, and recurrent epidemics caused by mutant strains have swept the world again since 2010. In this study, single-cell RNA sequencing was used perform first time, our knowledge, a systematic analysis of pig jejunum infected PEDV. Pig intestinal cell types were identified representative markers new tuft marker, DNAH11. Excepting enterocyte cells, goblet cells confirmed Enrichment...
Coronaviruses (CoVs) comprise a group of important human and animal pathogens. Despite extensive research in the past 3 years, host innate immune defense mechanisms against CoVs remain incompletely understood, limiting development effective antivirals non-antibody-based therapeutics. Here, we performed an integrated transcriptomic analysis porcine jejunal epithelial cells infected with epidemic diarrhea virus (PEDV) identified cytidine/uridine monophosphate kinase 2 (CMPK2) as potential...
Group A porcine rotavirus (RVA) is a serious threat to the breeding industry worldwide, which was associated with severe diarrhea in piglets. However, prevalence and molecular characterizations of RVA circulating farms East China remains largely unknown. Five hundred ninety-four samples were collected from 35 September 2017 December 2019. The results showed that 16.8% positive for all samples. Among different types samples, highest rate intestinal (19.5%), among pigs at growth stages,...
Abstract Background Mycoplasma hyopneumoniae is the causative agent of porcine enzootic pneumonia (EP), a mild, chronic swine. Despite presenting with low direct mortality, EP responsible for major economic losses in pig industry. To identify virulence-associated determinants M. , we determined whole genome sequence strain 168 and its attenuated high-passage 168-L carried out comparative genomic analyses. Results We performed first comprehensive analysis hyopneumonia e made preliminary...
Porcine epidemic diarrhea (PED) re-emerged in China late 2010 and has now become widespread. Accumulated evidence indicates that this large-scale outbreak of was caused by variants the highly virulent porcine virus (PEDV). A pandemic PEDV YC2014 strain (YC2014) isolated from clinical samples. An iTRAQ-based comparative quantitative proteomic study IPEC-J2 cells infected with a classical CV777 (CV777) performed to determine differences between infection. Totals 353 299 differentially...
African swine fever (ASF) is a highly contagious hemorrhagic viral disease of domestic and wild pigs all breeds ages, caused by virus (ASFV). Due to the absence safe efficacious vaccine, accurate laboratory diagnosis critical for control ASF prevention. The p30 protein immunogenic stimulates high level antibody response ASFV infection. We developed panel 4 monoclonal antibodies (mAbs) against protein, mAb-2B4 showed highest percent inhibition (PI) 70% in solid phase blocking ELISA (bELISA)....
Porcine rotaviruses (PoRVs) cause severe economic losses in the swine industry. P[7] and P[23] are predominant genotypes circulating on farms, but no vaccine is yet available. Here, we developed a bivalent subunit PoRV using truncated versions (VP4*) of VP4 proteins from P[23]. The vaccination mice with elicited more robust neutralizing antibodies (NAbs) cellular immune responses than its components, even at high doses. inactivated prepared strains PoRVs G9P[7] G9P[23] were used to examine...
// Jianxin Chen 1,* , Mubing Duan 2,* Yaqin Zhao 1,4 Fangfang Ling 1 Kun Xiao 2 Qian Li Bin 2,3 Chunni Lu Wenbao Qi Zhenling Zeng Ming Liao Yahong Liu and Weisan Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development Safety Evaluation, College Medicine, South China Agricultural University, Guangzhou, Department Biochemistry Genetics, La Trobe Institute for Molecular Science, Melbourne, Victoria, Australia 3 National Engineering Research Center Immunological Products,...
Abstract Porcine respiratory disease complex (PRDC) is one of the most challenging health concerns for pig production worldwide. The aim present study was to determine prevalence pathogens associated with PRDC, including porcine reproductive and syndrome virus (PRRSV) circovirus type 2 (PCV2) bacterial agents, such as Streptococcus suis , Haemophilus parasuis Actinobacillus pleuropneumoniae in clinically healthy pigs Eastern China. Molecular detection revealed positive single‐pathogen rates...
Porcine deltacoronavirus (PDCoV) can cause diarrhea and dehydration in newborn piglets. Here, we developed a double antibody sandwich quantitative enzyme-linked immunosorbent assay (DAS-ELISA) for detection of PDCoV by using specific monoclonal against the N protein an anti-PDCoV rabbit polyclonal antibody. Using DAS-ELISA, limit recombinant virus titer were approximately 0.5 ng/mL 103.0 TCID50/mL, respectively. A total 59 intestinal 205 fecal samples screened presence DAS-ELISA reverse...