A5082501842- Connexins and lens biology
- Intraocular Surgery and Lenses
- Anorectal Disease Treatments and Outcomes
- Heparin-Induced Thrombocytopenia and Thrombosis
- Corneal surgery and disorders
- Connective tissue disorders research
- Glaucoma and retinal disorders
- Atherosclerosis and Cardiovascular Diseases
- Cell Adhesion Molecules Research
- Wnt/β-catenin signaling in development and cancer
- Cardiac Health and Mental Health
- Surgical Sutures and Adhesives
- Cell Image Analysis Techniques
- Dupuytren's Contracture and Treatments
- Urinary Bladder and Prostate Research
- Aldose Reductase and Taurine
- Advanced Fluorescence Microscopy Techniques
- Ocular Infections and Treatments
- Cerebrovascular and genetic disorders
- Cerebrovascular and Carotid Artery Diseases
University of Delaware
2018-2024
Western blotting (WB), enzyme-linked immunosorbent assay (ELISA) and flow cytometry (FC) have long been used to assess quantitate relative protein expression in cultured cells tissue samples. However, WB ELISA limited ability meaningfully levels tissues with complex cell composition, while dissociation followed by FC is not feasible when limiting and/or difficult isolate. While detection using immunofluorescent (IF) probes has traditionally considered a qualitative technique, advances probe...
Purpose: To determine cataract surgeon viewpoints on the efficacy of available therapies/preventatives for two common sequelae surgery: inflammation and posterior capsular opacification (PCO). Methods: Cataract surgeons practicing worldwide specializing in adult, pediatric veterinary patients were interviewed between March August 2018. Results: Ocular following surgery is treated by either corticosteroids and/or nonsteroidal anti-inflammatories (NSAIDs). Adult are satisfied with current...
Fibrotic posterior capsular opacification (PCO), a major complication of cataract surgery, is driven by transforming growth factor–β (TGF-β). Previously, αV integrins were found to be critical for the onset TGF-β–mediated PCO in vivo; however, functional heterodimer was unknown. Here, β8 integrin–conditional knockout (β8ITG-cKO) lens epithelial cells (LCs) attenuated their fibrotic responses, while both β5 and β6 integrin–null LCs underwent changes similar WT at 5 days post surgery (PCS)....
Cataracts are treated by lens fiber cell removal followed intraocular (IOL) implantation into the capsule. While effective, this procedure leaves behind numerous epithelial cells (LECs) which undergo a wound healing response that frequently leads to posterior capsular opacification (PCO). In order elucidate acute of LECs models cataract surgery (post surgery, PCS), RNA-seq was conducted on derived from wild type mice at 0 and 6 h PCS. This analysis found upregulate expression proinflammatory...
The late embryonic mouse lens requires the transcription factor ATF4 for its survival although underlying mechanisms were unknown. Here, RNAseq analysis revealed that E16.5 Atf4 null lenses downregulate mRNA levels of epithelial markers as well known fiber cell differentiation. However, a comparison this list differentially expressed genes (DEGs) with other transcriptional regulators development indicated expression is not directly controlled by previously described gene regulatory network....