A5065593723- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Epigenetics and DNA Methylation
- SARS-CoV-2 and COVID-19 Research
- RNA modifications and cancer
- Biosensors and Analytical Detection
- MicroRNA in disease regulation
- Viral gastroenteritis research and epidemiology
- Telomeres, Telomerase, and Senescence
- Advanced Technologies in Various Fields
- Protein Degradation and Inhibitors
- Model Reduction and Neural Networks
- Wireless Communication Networks Research
- Ideological and Political Education
- Bacteriophages and microbial interactions
- Hybrid Renewable Energy Systems
- CAR-T cell therapy research
- Cancer-related gene regulation
- Extracellular vesicles in disease
- Brain Tumor Detection and Classification
- Fluid Dynamics and Vibration Analysis
- Industrial Vision Systems and Defect Detection
- Viral Infections and Outbreaks Research
- Alcohol Consumption and Health Effects
Jinan University
2022-2025
University of Calgary
2024
First Affiliated Hospital of Jinan University
2023-2024
Guilin University of Electronic Technology
2024
Shanghai University
2023
Guangdong University of Foreign Studies
2023
Henan University of Science and Technology
2023
First Affiliated Hospital of Bengbu Medical College
2023
University of Stuttgart
2023
Union Hospital
2016-2019
Abstract Viral infection in respiratory tract usually leads to cell death, impairing function cause severe disease. However, the diversity of clinical manifestations SARS-CoV-2 increases complexity and difficulty viral prevention, especially high-frequency asymptomatic risk virus transmission. Studying how affects apoptotic pathway may help understand pathological process its infection. Here, we uncovered imployed a distinct anti-apoptotic mechanism via N protein. We found virus-like...
Single image super-resolution (SISR) refers to the reconstruction from corresponding low-resolution (LR) input a high-resolution (HR) image. However, since single corresponds multiple images, this is an ill-posed problem. In recent years, generative model-based SISR methods have outperformed conventional in performance. based on GAN, VAE, and Flow problems of unstable training, low sampling quality, expensive computational cost. These models also struggle achieve trifecta diverse,...
On target: We have developed two cascade amplification strategies that combine duplex specific nuclease (DSN) amplicon with either G-quadruplex-based DNA peroxidase or 8–17 DNAzyme for miRNA detection. In this way, sensitive and convenient detection of miRNAs was achieved. the peroxidase-based system, a visual color change could be observed in presence target (see scheme). As service to our authors readers, journal provides supporting information supplied by authors. Such materials are peer...
The analysis of DNA methylation and MTase (methyltransferase) activity is important in epigenetic study. We have developed a novel strategy for sensitive based on hairpin shaped DNAzyme; 8-17 DNAzyme amplicon has been adopted found to be very effective such analysis.
Abstract Global coronavirus disease 2019 (COVID‐19) pandemics highlight the need of developing vaccines with universal and durable protection against emerging SARS‐CoV‐2 variants. Here we developed an extended‐release vaccine delivery system (GP‐diABZI‐RBD), consisting original WA1 strain receptor‐binding domain (RBD) as antigen diABZI stimulator interferon genes (STING) agonist in conjunction yeast β‐glucan particles (GP‐diABZI) platform. GP‐diABZI‐RBD could activate STING pathway inhibit...
The viral protein mutations can modify virus-host interactions during virus evolution, and thus alter the extent of infection or pathogenicity. Studies indicate that nucleocapsid (N) SARS-CoV-2 participates in genome assembly, intracellular signal regulation immune interference. However, its biological function evolution is not well understood. N were analyzed Delta, Omicron, original strains. Two with a methionine (M) residue at site 203 tyrosine (Y) 377 found Delta strain but Omicron...
Exosomal microRNAs (miRNAs) serve as dependable and noninvasive biomarkers for early cancer diagnosis. However, the accurate feasible detection of exosomal miRNAs is often hindered by their low abundance requirement specialized equipment miRNA detection. In this study, we present a novel approach, termed primer exchange reaction-based fluorescence emission with cascade RNA aptamers transcription (PERFECT) highly sensitive miRNA. The design method involves selective interaction DNA probe...
We reported an efficient strategy based on a strand displacement amplification for serum miRNA detection. In such system, multiplexed, sensitive and quick detection of miRNAs could be achieved through combination fluorescence labeled probes, common primer polymerase. This potentially used in the clinical field to achieve early disease diagnosis prognosis.
Abstract Non‐viral vector chimeric antigen receptor (CAR)‐T cells have garnered increasing attention due to their ability efficiently eradicate cancer while mitigating undesirable side effects. However, the current methods for engineering T (CAR‐T) employ viral vectors that result in permanent CAR expression and potentially severe negative impacts. As a solution these challenges, triggering transitory of CARs via messenger RNA (mRNA) has emerged as promising strategy. Currently,...
The present study demonstrated a highly sensitive strategy for measuring telomerase activity in cell extracts. Furthermore, we applied the new situ detection of at cellular level cancer cells, together with normal as negative control.
A squarylium dye TSQ1 shows a remarkable fluorescence enhancement selectivity for the G-quadruplex DNA structure against duplex DNA, it can be distinguished even by naked eye. What is more important, did not induce G-rich sequence folding into which means used to detect structures in cells.
N6-Methyladenosine (m6A) represents a relatively abundant modification in eukaryotic RNA. Because m6A has similar properties to adenosine and low reactivity, limited research been focused on this nucleoside. In study, we revealed an important intermediate the oxidation of through bicarbonate-activated peroxide system. Over course oxidation, found new mechanism which N6-hydroxymethyladenosine (hm6A), N6-formyladenosine (f6A) N6-hydroperoxymethyladenosine (oxm6A) were products, was final...
8-oxoguanine DNA glycosylase can maintain genomic stability and integrity. However, it interfere with the regular damage repair process, possibly leading to development of cancer various other human diseases when its activity becomes abnormal. Current methods for detecting often suffer from low sensitivity, time-consuming procedures, labor-intensive requirements, need specialized equipment trained professionals execution. Consequently, there is an urgent a portable, user-friendly assay that...
Impacts of climate change on
We report a new biofunctionalized nanoplatform based on hyaluronic acid-coated gold-nano-dot-decorated hollow carbon nanospheres (AuHCNs-HA) for microRNA imaging in living cells. Importantly, the HA-coated could be internalized into target cells via CD44 receptor-mediated endocytosis. It can further applied intracellular miR-21 CD44-positive colorectal cancer
Quantitative information about DNA sequences with diverse methylation statuses can be provided through fluorescein-dGTP incorporated asymmetric PCR assay.
Here, we first demonstrated that 5-MedCTP could be incorporated into the synthetic DNA template by exonuclease deficient Klenow fragment with a much higher efficiency than dCTP and 5-hydroxymethyl-dCTP. Further, conducted comparable study of primer extension reaction using templates containing deoxycytidine (dC) or 5-methyldeoxycytidine (5-mdC) for incorporating different triphosphates. Based on our findings, enhance substrate activity (exo-) this feature potentially used in methylation analysis.
Early cancer diagnosis is of great significance to relative prevention and clinical therapy, it crucial efficiently recognize cancerous tumor sites at the molecular level. Herein, we proposed a versatile efficient strategy based on aptamer recognition photoactivation imaging for diagnosis. This first time that visible light-controlled photoactivatable aptamer-based platform has been applied The can accurately detect nucleolin-overexpressed cells be used highly selective cell screening tissue...