A5017422157- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Salmonella and Campylobacter epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotics Pharmacokinetics and Efficacy
- Bacillus and Francisella bacterial research
- Antibiotic Use and Resistance
- Urinary Tract Infections Management
- Escherichia coli research studies
- Streptococcal Infections and Treatments
- Bacterial Identification and Susceptibility Testing
- Yersinia bacterium, plague, ectoparasites research
- Viral gastroenteritis research and epidemiology
- Bacterial biofilms and quorum sensing
- Toxin Mechanisms and Immunotoxins
- Genomics and Phylogenetic Studies
- Surgical site infection prevention
- Microbial infections and disease research
- Antimicrobial Resistance in Staphylococcus
- Aquaculture disease management and microbiota
- Identification and Quantification in Food
- Parasitic Infections and Diagnostics
- Bacterial Genetics and Biotechnology
- Neonatal and Maternal Infections
- Biochemical and Structural Characterization
Chinese Academy of Agricultural Sciences
2021-2023
Jilin University
2021-2023
Sierra Leone Urban Research Centre
2022-2023
Jilin Academy of Agricultural Sciences
2022
Academy of Military Medical Sciences
2008-2020
China Agricultural University
2018
Westmead Institute for Medical Research
2016
Westmead Hospital
2014-2016
The University of Sydney
2014-2016
Changchun University of Chinese Medicine
2015
Abstract Background There is limited information on surgical site infections (SSI) and the related antibiotic resistance needed to guide their management prevention in Sierra Leone. In this study, we aimed establish incidence risk factors of SSI among adults attending a tertiary hospital, secondary health facility Freetown, Methods This prospective cohort study designed collect data from adult (18 years or older) patients who attended elective emergency surgeries at two hospitals Freetown...
Migratory birds play an important role in the spread of multidrug-resistant (MDR) bacteria. To investigate prevalence MDR Escherichia coli migratory China and potential relationships with environment, a total 1387 samples (fecal samples, cloacal swabs, or throat swabs) were collected from three different river basins China. The processed subjected to bacteriological examinations. Antimicrobial susceptibility testing recovered isolates was performed using E-test for detection minimum...
Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli have been frequently isolated from food-producing animals and pose a serious threat to human health. This study collected 195 ESBL-producing E. isolates 20 chicken farms 3 live-bird markets located in Northeast China (Heilongjiang, Liaoning, Jilin) Jiangsu province February 2011 October 2013. ESBL genes, including blaCTX-M, blaTEM, blaSHV, were detected characterized, the susceptibilities of these strains various antimicrobial...
Abstract Background Available data on antibiotic resistance in sub-Saharan Africa is limited despite its increasing threat to global public health. As there no previous study patients with clinical features of healthcare-associated infections (HAIs) Sierra Leone, research needed inform health policies. Our aimed assess rates from isolates the urine and sputum samples HAIs. Methodology We conducted a cross-sectional adult inpatients aged ≥18 years at Connaught Hospital, an urban tertiary care...
(1) Background: there has been a growing concern about pet-spread bacterial zoonosis in recent years. This study aimed to investigate the trend drug-resistance of canine Escherichia coli isolates northeast China between 2012-2021 and differences E. different origins 2021. (2) Methods: were isolated from feces or anal swab samples dogs cats, their antibiotic susceptibility profiles phylogenetic grouping identified. PCR was applied on extended spectrum β-lactamase (ESBL) for resistance genes....
The minimal concentration of antibiotic required to inhibit the growth different isolates a given species with no acquired resistance mechanisms has normal distribution. We have previously shown that presence or absence transmissible genes excellent predictive power for phenotype. In this study, we analyzed distribution six β-lactam susceptibility phenotypes associated commonly in Enterobacteriaceae Sydney, Australia. Escherichia coli (n = 200) and Klebsiella pneumoniae 178) clinical...
Although hand hygiene (HH) is the most effective intervention to reduce spread of infections, there are limited data on HH facilities, policy, and compliance in sub-Saharan Africa. This cross-sectional study aimed at assessing using WHO self-assessment framework, technical reference manual, a modified infection control tool two hospitals Sierra Leone. Only 10% 9% regional capital city had running tap water, respectively. Veronica buckets were resources for 89% units hospital 92% hospital....
Mobile colistin resistance (mcr) gene mcr-10.1 has been distributed widely since it was initially identified in 2020. The aim of this study to report the first Africa and mcr Sierra Leone; furthermore, we presented diverse modular structures loci. Here, complete sequence one mcr-10.1-carrying plasmid clinical Enterobacter cloacae isolate from Leone determined. Detailed genetic dissection comparison were applied plasmid, together with a homologous carrying GenBank. Moreover, 19 loci...
Streptococcus suis serotype 2 (SS2) is an important pathogen that affects pigs. However, neither its virulence nor pathogenesis of infection has yet to be fully elucidated. The present study identifies a novel virulence‑associated protein E gene (vapE) SS2. To investigate the importance vapE in SS2 infection, knock‑out mutant based on wild‑type strain ZY458 was designated 458ΔvapE. 458ΔvapE generated through homologous recombination, using combined plasmid with fragment and pSET4s suicide...
Despite the impact of inappropriate prescribing on antibiotic resistance, data surgical prophylaxis in sub-Saharan Africa are limited. In this study, we evaluated use and consumption 4 hospitals located 2 geographic regions Sierra Leone.We used a prospective cohort design to collect from patients aged 18 years or older between February October 2021. Data were analyzed using Stata version 16 software.Of 753 patients, 439 (58.3%) females, 723 (96%) had received at least 1 dose antibiotics....
This study aimed to characterize the antimicrobial resistance and virulence of Enterococcus from dogs cats in Northeast China evaluate its zoonotic risk based on a total 469 enterococci strains 610 samples, including 238 E. faecium 128 faecalis. The isolation rate police dog samples was 93.79%, pet 69.90% cat 76.67%. differences prevalence faecalis among different hosts were statistically significant (P<0.05). assays showed that most genes detected existed carried least number genes....
Abstract Objective Catheter-associated urinary tract infections (CAUTI) are common worldwide, but due to limited resources, its actual burden in low-income countries is unknown. Currently, there gaps knowledge about CAUTI lack of surveillance activities Sierra Leone. In this prospective cohort study, we aimed determine the incidence and associated antibiotic resistance two tertiary hospitals different regions Results The mean age 459 recruited patients was 48.8 years. majority were females...
Migratory birds are recently recognized as Vibrio disease vectors, but may be widespread transporters of strains. We isolated cholerae (V. cholerae) and metschnikovii metschnikovii) strains from migratory bird epidemic samples 2017 to 2018 V. feces in 2019 taken the Inner Mongolia autonomous region China. To investigate evolution these two species, we sequenced genomes 40 34 compared with reference strain genomes. The pan-genome all was large, exhibiting considerable individual differences....
Background Carbapenem-resistant Klebsiella pneumoniae (CRKP) infection is a serious problem in hospitals worldwide. We monitored tertiary hospital Changchun, Jilin Province, China, and found that CRKP was the major species among carbapenem-resistant isolates sewage. Subsequently, we evaluated drug susceptibility, resistance genes, virulence outer pore membrane protein-related genes ( OmpK35 & OmpK 36 ), multi-locus sequence typing replicons, biofilm formation capabilities, to...
The presence of multidrug-resistant bacterial pathogens in the environment poses a serious threat to public health. opportunistic Acinetobacter spp. are among most prevalent causes nosocomial infections. Here, we performed complete genome sequencing calcoaceticus strain XM1570, which was originally cultivated from sputum patient diagnosed with pneumonia Xiamen 2010. We identified carbapenem resistance associated gene blaNDM-1 located on 47.3-kb plasmid. Three methods – natural reproduction,...
This study aimed to evaluate antimicrobial resistance, virulence, and the genetic diversity of Aeromonas isolated from migratory birds Guangxi Province, Guangdong Ningxia Hui Autonomous Region, Jiangxi Inner Mongolia in China. A total 810 samples were collected, including fresh feces, cloacal swabs, throat swabs. The collected processed subjected bacteriological examination. resistance 21 antibiotics was evaluated. phylogenetic tree constructed using concatenated...
Objectives: To characterize the antimicrobial resistance and virulence of pathogenic Escherichia coli isolated from diseased captive giant pandas. Methods: Antimicrobial susceptibility minimum inhibitory concentration (MIC) were determined by broth dilution method. Whole-genome sequencing was used to phylogeny, serotype, virulence, resistome, plasmids, genetic structures cefotaxime (CTX)-M genes. Results: Four extended-spectrum beta-lactamase (ESBL)-producing E. strains identified MICs...
Anthrax is an acute zoonotic infectious disease caused by the bacterium known as Bacillus anthracis. From 26 July to 8 August 2015, outbreak with 20 suspected cutaneous anthrax cases was reported in Ganquan County, Shaanxi province China. The genetic source tracking analysis of performed molecular epidemiological methods this study.Three typing methods, namely canonical single nucleotide polymorphisms (canSNP), multiple-locus variable-number tandem repeat (MLVA), and (SNR) analysis, were...
ABSTRACT Here, a complete genome sequence of Wohlfahrtiimonas chitiniclastica strain BM-Y is presented. The whole 2.18-Mb and contains bla VEB-1 gene cassette which endows it with resistance to ceftazidime, ampicillin, tetracycline, etc. To our knowledge, this the first time that an extended spectrum beta-lactamase (ESBL) type W. has been found.