A5100339964- Cancer-related molecular mechanisms research
- Dental materials and restorations
- MicroRNA in disease regulation
- Dental Erosion and Treatment
- Antibiotic Resistance in Bacteria
- Autophagy in Disease and Therapy
- Molecular Biology Techniques and Applications
- Ubiquitin and proteasome pathways
- Dental Trauma and Treatments
- Circular RNAs in diseases
- Pharmaceutical and Antibiotic Environmental Impacts
- Endoplasmic Reticulum Stress and Disease
- Endodontics and Root Canal Treatments
- Cancer-related Molecular Pathways
- Cancer Research and Treatments
- Microtubule and mitosis dynamics
- 14-3-3 protein interactions
- Retinoids in leukemia and cellular processes
- RNA modifications and cancer
- Antibiotic Use and Resistance
- Dental Anxiety and Anesthesia Techniques
- Vibrio bacteria research studies
- Single-cell and spatial transcriptomics
- Calcium signaling and nucleotide metabolism
- Animal Behavior and Reproduction
Stomatology Hospital
2013-2025
Zhejiang University
2013-2025
Jilin University
2006-2023
Chinese Academy of Agricultural Sciences
2021-2023
Henan University Huaihe Hospital and Huaihe Clinical Institute
2021
Jilin Medical University
2018
Second Affiliated Hospital of Zhengzhou University
2005-2018
Jiangsu University
2015
National Center of Biomedical Analysis
2011-2013
Zhejiang University Medical College Affiliated Stomatological Hospital
2010
Autophagy is a self‐degrading process that triggered by diverse stimuli including ionizing radiation. In this study we show novel phenomena in which transfection of miR‐199a‐5p mimic significantly suppresses IR‐induced autophagy MCF7 cells, and up‐regulates basal MDA‐MB‐231 breast cancer cells. We also identify DRAM1 Beclin1 as target genes for miR‐199a‐5p. Overexpression inhibits expression while it enhances these Furthermore, sensitizes cells to irradiation. Therefore, our data unique...
Migratory birds play an important role in the spread of multidrug-resistant (MDR) bacteria. To investigate prevalence MDR Escherichia coli migratory China and potential relationships with environment, a total 1387 samples (fecal samples, cloacal swabs, or throat swabs) were collected from three different river basins China. The processed subjected to bacteriological examinations. Antimicrobial susceptibility testing recovered isolates was performed using E-test for detection minimum...
Purpose: Cell death is one of the most important endpoints radiosensitivity. The tumor suppressor p53 participates not only in regulation apoptosis, but also autophagy mechanism. In this study, H1299-P53 (with wild-type p53) and H1299-175H mutant 175H) were used, effects on radiosensitivity analyzed. Methods: models with different status established by gene engineering, cell viability was examined colony formation assay, counting kit-8 (CCK-8), 3-Methyladenine, Z-VAD used to block...
Clinical Relevance Dentin desensitizers and one-bottle self-etching adhesives can significantly reduce dentin hypersensitivity. desensitizing effectiveness depends on the individual desensitizers/adhesives used.
Cisplatin is a commonly used drug for chemotherapy, however, whether it may be synergistically with radiotherapy remains unclear. The present study investigated the underlying mechanisms of synergistic killing by radiosensitization and cisplatin, focus on growth inhibition, apoptosis autophagy non‑small cell human lung cancer cells in vitro tumor xenograft vivo. A549 were experiments divided into following four treatment groups: Sham‑irradiated; conventional (CRT) five doses 2 Gy every day;...
(1) Background: there has been a growing concern about pet-spread bacterial zoonosis in recent years. This study aimed to investigate the trend drug-resistance of canine Escherichia coli isolates northeast China between 2012-2021 and differences E. different origins 2021. (2) Methods: were isolated from feces or anal swab samples dogs cats, their antibiotic susceptibility profiles phylogenetic grouping identified. PCR was applied on extended spectrum β-lactamase (ESBL) for resistance genes....
Abstract Purpose Autophagy has attracted attentions as a novel mechanism for tumor development. In this study Human ovarian carcinoma cell line SKOV3 and multidrug-resistant phenotype SKVCR cells were used the roles of autophagy in radiation-induced death analyzed. Methods materials Cell viability was examined by colony formation counting kit-8 (CCK-8) assay, 3MA ZVAD to block apoptosis, respectively. Quantitative real-time PCR detect mRNA level Western blot protein expression,...
Abstract Atherosclerosis (AS) is the main aetiology of coronary heart disease, cerebral infarction and peripheral vascular disease in humans. Long‐noncoding RNA (LincRNA)‐p21 has been reported to participate development AS. Therefore, this study was designed investigate mechanism LincRNA‐p21 on suppressing We fed ApoE −/− mice with a high‐fat diet induce an AS mouse model where lesion area extent lipid deposition were measured. The binding miR‐221 or SIRT1 measured using dual luciferase...
Clinical Relevance Salivary contamination before and after priming could significantly reduce the enamel bond strength of self-etching adhesives. Proper isolation should be performed during application adhesives placement resin composite. Thorough water-spraying improve μTBS saliva-contaminated enamel.
DNA damage triggers cell cycle arrest to provide a time window for repair. Failure of could lead genomic instability and tumorigenesis. damage-induced G 1 is generally achieved by the accumulation Cyclin-dependent kinase inhibitor (p21). However, p21 degraded does not play role in UV-induced arrest. The mechanism thus remains elusive. Here, we have identified critical CUE domain-containing protein 2 (CUEDC2) this process. CUEDC2 binds inhibits anaphase-promoting complex/cyclosome-Cdh1 (APC/C...
To study antimicrobial resistance and virulence genes of Klebsiella pneumoniae Raoultella strains isolated from captive giant pandas. Non-duplicate fecal samples were collected 128 pandas during 2017–2019. All microbial tested for drug susceptibility using BD verification panels. Four extended-spectrum β-lactamase genes, nine six capsular serotype detected PCR. 42 K . different Antibiotic rates 1.9%–23.5%, except ampicillin, 7.8% the isolates multidrug-resistant to 7–10 antibiotic classes....
Multi-drug-resistant Escherichia coli, Pseudomonas aeruginosa, and Enterococcus faecalis were isolated from the tissue samples of a giant panda. The E. coli strain SH-YH-DH that we carried self-transferable plasmid associated with multi-drug-resistant genes ( blaCTX-M-55, blaTEM-1, sul1, floR, strB, aac(6')-Ib, tetA/R). This exhibited phenotypic resistance to gentamicin, cefotaxime, ampicillin, chloramphenicol, trimethoprim-sulfamethoxazole, tetracycline. We report participation in...
Atrazine (ATZ), a widely used triazine herbicide, has been detected in the surface and ground water even far from where it is applied. Recently, biotoxicity of atrazine to immune, reproductive endocrine systems preliminarily observed laboratory experiments epidemiological research studies. In order further comprehend carcinogenic nature ATZ, vitro vivo models were established this study explore effects ATZ exposure on hepatocellular carcinoma. The results showed that after being treated with...
Targeting multiple signaling pathways is a potential novel therapeutic strategy for the treatment of leukemias. Leukemia cells express high levels sphingosine kinase 1 (Sphk1) and sirtuin (SIRT1). However, to best our knowledge, their interaction synergistic inhibitory effects on growth survival leukemia have not been investigated. The present study revealed role Sphk1/S1P/SIRT1 axis in K562, KCL22 TF1 hypothesized that inhibition Sphk1 SIRT1 had cells. Cell viability was tested using...
The aim of the present study is to investigate effect a recombinant plasmid adenovirus (pAd) expressing human interferon-λ1 (hIFN-λ1) on proliferation gastric adenocarcinoma cell line SGC‑7901. For this purpose, SGC-7901 cells were infected with pAd-hIFN-λ1, pAd-LacZ and phosphate‑buffered saline (PBS), respectively, subsequent effects compared. Cell was evaluated by MTT assay, while mRNA protein expression hIFN‑λ1 detected reverse transcription‑polymerase chain reaction analysis...
Purpose: To investigate the effects of phosphoric acid esters (PAEs)-containing primers on micro-tensile bond strengths (MTBS) etched enamel, micro-morphologies resin–enamel interfaces, and enamel surfaces.Materials Methods: Thirty-three bovine incisors were used in this study. After labial surfaces highly polished, they etched, water sprayed, air-dried. Afterward, treated with or without (control) one three PAEs-containing (40%MDP, Ivoclar Vivadent; Resulcin AquaPrime A + B, DMG; Xeno V,...