A5073440190- Virus-based gene therapy research
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA regulation and disease
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- interferon and immune responses
- SARS-CoV-2 and COVID-19 Research
- Cancer Research and Treatments
- Adenosine and Purinergic Signaling
- Respiratory viral infections research
- RNA Research and Splicing
- Tissue Engineering and Regenerative Medicine
- Intestinal Malrotation and Obstruction Disorders
- Cell Adhesion Molecules Research
- Nicotinic Acetylcholine Receptors Study
- Rabies epidemiology and control
- MicroRNA in disease regulation
- Angiogenesis and VEGF in Cancer
- Cytokine Signaling Pathways and Interactions
- Intracranial Aneurysms: Treatment and Complications
- Pressure Ulcer Prevention and Management
- Vascular Malformations Diagnosis and Treatment
- Fibroblast Growth Factor Research
- Influenza Virus Research Studies
Affiliated Hospital of Jiangsu University
2012-2024
Jiangsu University
2011-2024
Southeast University
2024
Weatherford College
2024
Central People's Hospital of Zhanjiang
2009
// Wenkang Luan 1, * , Lubo Li 2, Yan Shi 4, Xuefeng Bu 3 Yun Xia 1 Jinlong Wang Henry Siaw Djangmah Xiaohui Liu Yongping You 5 Bin Xu Department of Plastic Surgery, Affiliated People's Hospital Jiangsu University, Zhenjiang, Jiangsu, China 2 Neurosurgery, General 4 Nanjing First Hospital, Medical Nanjing, The These authors have contributed equally to this work Correspondence to: Luan, email: luanwenkang@126.com Xu, xubinfen@126.com Keywords: MALAT1, miR-22, MMP14 and Snail, ceRNA, melanoma...
Abstract Background and Objective Octamer‐4 (Oct4), a transcription factor involved in regulating human embryonic stem cells (ESCs), may play role tumorigenesis. Since little is known about the efficacy of Oct4 as potential biomarker for gastric cancer (GC), we investigated its expression GC tissues relationship to various clinicopathological parameters. Methods Primary tumor matching, adjacent non‐cancerous were obtained from 62 patients, was examined by reverse transcription‐PCR (RT‐PCR)...
Abstract Background Long intergenic non-protein coding RNA 520 (LINC00520), a novel identified lncRNA, has been shown to modulate the malignant phenotype of tumor cells in some tumors. However, exact role and molecular mechanism LINC00520 melanoma not studied. Methods The expression tissues were detected by using RNA-seq analysis qRT-PCR. Melanoma cases from public databases (The Cancer Genome Atlas (TCGA), GEO#GSE15605, GEO#GSE34460 GEO#GSE24996) included this study. CCK-8 assay, EdU...
An increasing number of microRNAs have been found to be involved in tumorigenesis, including melanoma tumorigenesis. miR-204-5p is down-regulated and functions as a tumor suppressor many human malignant tumors. expression also decreased tissues, but its biological roles molecular mechanisms remain unclear. In this study, the aberrant down-regulation was detected melanoma, especially metastatic melanoma. served protective factor for prognosis patients. We determined that suppresses cell...
The long noncoding RNA (lncRNA) OIP5-AS1 has been considered to promote the growth and metastasis of many human tumors. However, role in melanoma not reported. In this study, we found that levels were significantly elevated tissue high expression was an independent risk factor for poor survival patients with melanoma. miR-217 suppressed glutamine catabolism cells by targeting glutaminase (GLS), rate-limiting enzyme catabolism. We also demonstrated acted as a sponge upregulate GLS expression,...
The aim of this study were to investigate the possible pro-apoptotic mechanisms recombinant Newcastle disease virus (NDV) strain rL-RVG, which expresses rabies glycoprotein, in A549 lung adenocarcinoma cells via regulation alpha 7 nicotinic acetylcholine receptors (α7 nAChRs) and analyze relationships between α7 nAChR expression cancer clinical pathological features. A549, LΑ795, small-cell carcinoma (SCLC) cells, among others, was detected using reverse transcription polymerase chain...
Newcastle disease virus (NDV) is a member of the genus Avulavirus in Paramyxoviridae family and its antitumor properties depend on ability to kill malignant cells while not affecting normal cells. The present study investigated recombinant avirulent NDV LaSota strain (wild‑type strain) expressing rabies glycoprotein (rL‑RVG), examined oncolytic effect lung adenocarcinoma A549 cell line evaluated potential serve as vaccine against cancer. were infected with rL‑RVG analyzed by MTT, western...
Sclerosing angiomatoid nodular transformation (SANT) of the spleen is an uncommon benign vascular lesion with obscure etiology. It predominantly affects middle-aged women and presents nonspecific clinical signs, making preoperative diagnosis challenging. The definitive SANT relies on pathological examination following splenectomy. This study aims to contribute understanding by presenting a case series reviewing literature highlight presentation, diagnostic challenges, treatment outcomes.
Abstract Type III interferon (IFN-λ) is a novel member of the family, which preferentially promotes antiviral responses from epithelial cells and cooperates with type I IFNs in clearance viral infections. However, effect mIFN-λ2 to LA795 lung adenocarcinoma cell largely unknown. In this study, we transfected Ad-mIFN-λ2 vector into tumor-bearing mice explore on proliferation immune response mice. Transfected by vector, significant decrease tumor growth, subcutaneous necrosis, cystic...
Background IFN‐λs are a kind of cytokine with anti‐tumor, immunomodulatory, and anti‐proliferative activity. Recent studies have shown that the recombinant Newcastle disease virus expresses human IFN‐λ1 (rL‐hIFN‐λ1), which plays role in gastric cancer cell apoptosis. Endoplasmic reticulum stress (ERS) induces autophagy apoptosis tumor cells. In this study, we explored relationship between ERS rL‐hIFN‐λ1‐induced lung adenocarcinoma A549 cells its underlying mechanism. Methods First,...
BACKGROUND The recombinant avirulent Newcastle disease virus (NDV) LaSota strain expressing the rabies glycoprotein (rL-RVG) can induce much greater apoptosis than NDV in gastric carcinoma cells, but mechanisms involved remains unclear. MATERIAL AND METHODS 2 cell lines were divided into rL-RVG group, and PBS group. MTT assay was used to detect analyze viability. siRNA for alpha7-nAChR, alpha7-nAChR antagonist, or agonist, AKT p-AKT agonist pretreatment. protein expressions of RVG, NDV,...
The aim of the present study is to investigate effect a recombinant plasmid adenovirus (pAd) expressing human interferon-λ1 (hIFN-λ1) on proliferation gastric adenocarcinoma cell line SGC‑7901. For this purpose, SGC-7901 cells were infected with pAd-hIFN-λ1, pAd-LacZ and phosphate‑buffered saline (PBS), respectively, subsequent effects compared. Cell was evaluated by MTT assay, while mRNA protein expression hIFN‑λ1 detected reverse transcription‑polymerase chain reaction analysis...
Abstract Acute purulent enteritis is uncommon, which occasionally occurs in association with mechanical injuries from foreign bodies of helminth parasites and the contaminated food, leading to bacterial invasion. Herein we report a case acute after appendectomy. A 44-year-old male was diagnosed right lower abdominal pain vomiting. appendicitis diagnosed, an open appendectomy performed. Postoperatively, patient developed symptoms small intestine obstruction. laparotomy revealed necrosis...
Circular RNAs (circRNAs) have been found to be linked cancer progression and metastasis, but there is not much known about their connection lung adenocarcinoma (LAC). In the previous study reported by our group, has_circ_0002360 was highly expressed in LAC tissues. The goal of this investigate potential impact LAC.
To investigate the effects of integrin-β1 gene expression inhibited by shRNA on invasion pancreatic carcinoma PANC-1 cells in vitro.The eukaryotic plasmid short hairpin RNA (shRNA) targeting (integrin-β1-shRNA) was constructed and transfected into cells. The expressions mRNA protein were detected real-time quantitative polymerase chain reaction (PCR) western blot assay, respectively. invasive ability observed with a transwell cell culture chamber MMP-2 MMP-9 assayed.Compared to untransfected...
Background: Tumor-associated macrophages (TAMs) are frequently infiltrated in tumor microenvironment and promote progression. Lung cancer development largely depends upon the essential contributions from TAMs which generally polarize into M2 produce abundant anti-inflammatory factors facilitate development. The recombinant Newcastle disease virus expressing human IFN-λ1 (rL-hIFN-λ1) could regulate Th1/Th2 immune response to anti-tumor microenvironment. However, interaction between rL-hIFN-λ1...