A5018979358- Cell Adhesion Molecules Research
- Connective tissue disorders research
- TGF-β signaling in diseases
- Circadian rhythm and melatonin
- Skin and Cellular Biology Research
- Collagen: Extraction and Characterization
- Hedgehog Signaling Pathway Studies
- Liver Disease Diagnosis and Treatment
- Nitric Oxide and Endothelin Effects
- Cancer-related molecular mechanisms research
- Renal and related cancers
- Protease and Inhibitor Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Monoclonal and Polyclonal Antibodies Research
- Osteoarthritis Treatment and Mechanisms
- Hair Growth and Disorders
- Liver physiology and pathology
- Parathyroid Disorders and Treatments
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Eicosanoids and Hypertension Pharmacology
- Wnt/β-catenin signaling in development and cancer
- Proteoglycans and glycosaminoglycans research
- Reproductive System and Pregnancy
- Uterine Myomas and Treatments
Wakayama Medical University
2015-2024
Tokyo Women's Medical University
2012
Minoh City Hospital
2009
Yamagata University
2009
Kawasaki Medical School
2008
St. Mary's Hospital
1997
Heinrich Heine University Düsseldorf
1997
St Mary's Hospital
1997
University of Florence
1997
Harvard University
1989-1996
Tubulointerstitial fibrosis is the final common result of a variety progressive injuries leading to chronic renal failure. Transforming growth factor-β (TGF-β) reportedly upregulated in response injurious stimuli such as unilateral ureteral obstruction (UUO), causing associated with epithelial-mesenchymal transition (EMT) tubules and synthesis extracellular matrix. We now show that mice lacking Smad3 (Smad3ex8/ex8), key signaling intermediate downstream TGF-β receptors, are protected against...
Tubulointerstitial fibrosis is the final common result of a variety progressive injuries leading to chronic renal failure. Transforming growth factor-β (TGF-β) reportedly upregulated in response injurious stimuli such as unilateral ureteral obstruction (UUO), causing associated with epithelial-mesenchymal transition (EMT) tubules and synthesis extracellular matrix. We now show that mice lacking Smad3 (Smad3ex8/ex8), key signaling intermediate downstream TGF-β receptors, are protected against...
Hox genes regulate patterning during limb development. It is believed that they function in the determination of timing and extent local growth rates. Here, it demonstrated synpolydactyly, an inherited human abnormality hands feet, caused by expansions a polyalanine stretch amino-terminal region HOXD13. The homozygous phenotype includes transformation metacarpal metatarsal bones to short carpal- tarsal-like bones. mutations identify outside DNA binding domain HOXD13 as necessary for proper...
Type IX collagen is a nonfibrillar composed of three gene products, alpha 1(IX), 2(IX), and 3(IX). molecules are localized on the surface type II-containing fibrils consist two arms, long arm that crosslinked to II short projects into perifibrillar space. In hyaline cartilage, 1(IX) transcript encodes polypeptide with large N-terminal globular domain (NC4), whereas in many other tissues an alternative chain truncated NC4 domain. It has been proposed involved interaction each or components...
Synpolydactyly (SPD) is a dominantly inherited congenital limb malformation. Typical cases have 3/4 finger and 4/5 toe syndactyly, with duplicated digit in the syndactylous web, but incomplete penetrance variable expressivity are common. The condition has recently been shown to be caused by expansions of an imperfect trinucleotide repeat sequence encoding 15-residue polyalanine tract HOXD13. We studied 16 new 4 previously published SPD families, between 7 14 extra residues tract, analyze...
We have isolated overlapping cDNAs encoding the N-terminal non-triple-helical region of mouse alpha 1(XVIII) collagen and shown that three different variants exist. Each shows characteristic tissue-specific expression patterns. Immunohistochemical studies show positive staining for along basement membrane zones vessels in intestinal villi, choroid plexus, skin, liver, kidney. Thus, we conclude may interact (directly or indirectly) with components on basal surface endothelial/epithelial cells.
We have isolated overlapping mouse cDNAs encoding a collagenous polypeptide that we designated alpha 1(XVIII) collagen. Nucleotide sequence analysis shows collagen contains 10 triple-helical domains separated and flanked by non-triple-helical regions. Within the regions, there are several Ser-Gly-containing sequences conform to consensus for glycosaminoglycan attachment sites in proteoglycan core proteins. Northern blots show transcripts present multiple organs, with highest levels liver,...
Type IX collagen, containing molecules of the three distinct polypeptides alpha 1(IX), 2(IX), and 3(IX), is an interesting hybrid extracellular matrix component in cartilage eye tissues, with properties both a proteoglycan collagen. The 1 (IX) chain has two forms, as result tissue-specific utilization alternative promoters; 2(IX) carries covalently attached glycosaminoglycan side chain. We have introduced gene construct controlled by promoter/enhancer expressing truncated 1(IX) into mice....
A keloid is a specific skin lesion that expands beyond the boundaries of original injury as it heals. Histologically, characterized by excessive accumulation collagen. However, reasons for expansion and invasive nature keloids remain unknown.We evaluated collagen degradation migration cultured fibroblasts based on assumption these variables were functional relevance to expanding lesions.Collagen production was investigated detection type 1 (procollagen 1C peptide: P1P). Matrix...
We investigated whether aliskiren, a direct renin inhibitor, improves NO bioavailability and protects against spontaneous atherosclerotic changes. also examined the effects of cotreatment with aliskiren valsartan, an angiotensin II receptor blocker, on above-mentioned outcomes. Watanabe heritable hyperlipidemic rabbits were treated vehicle (control), or plus valsartan for 8 weeks. Then, acetylcholine-induced production was measured as surrogate index endothelium protective function, both...
The mitogen-activated protein kinase (MAPK) pathway allows cells to interpret external signals and respond appropriately, especially during the epithelial-mesenchymal transition (EMT). EMT is an important process embryonic development, fibrosis, tumor progression in which epithelial acquire mesenchymal, fibroblast-like properties show reduced intercellular adhesion increased motility. TGF- β signaling first be described as inducer of EMT, its relationship with Smad family already well...
Cardiac function is highly dependent on oxidative energy, which produced by mitochondrial respiration. Defects in are associated with both structural and functional abnormalities the heart. Here, we show that heart-specific ablation of circadian clock gene Bmal1 results cardiac defects include morphological changes abnormalities, such as reduced enzymatic activities within respiratory complex. Mice without a significant decrease expression genes fatty acid pathway, tricarboxylic cycle, chain...
Vascular calcification is a complication of diseases and conditions such as chronic kidney disease, diabetes, aging. Previous studies have demonstrated that high concentrations inorganic phosphate (Pi) can induce oxidative stress vascular smooth muscle cell calcification. KEAP1 (Kelch-like ECH-associated protein 1)/NF-E2-related factor 2 (NRF2) signaling has been shown to play important roles in protecting cells from stress. The current study aims investigate the possible involvement...
Mechanical strain reportedly stimulates the synthesis of collagen in vascular smooth muscle cells (SMCs). The present study was designed to investigate a possible involvement angiotensin II (Ang II) and transforming growth factor (TGF)-beta stretch-induced cultured SMCs derived from rabbit aortic media. were cyclically stretched at rate 10% elongation 30 cycles/min for 24 h using Flexercell unit (Flexcell International Corp., McKeesport, Pa.). A two-fold increase concurrent total protein...
Abstract We demonstrate that the gene encoding an extracellular matrix component contains two widely separated promoters controlling generation of different transcripts in a tissue-specific fashion. Two transcription start sites, about 20 kilobase pairs apart, alpha 1(IX) collagen are utilized to generate forms collagen-proteoglycan (collagen IX) chicken cartilage and cornea. Transcripts from upstream site encode large (266 amino acid residues) globular domain at terminus chains, whereas...