A5038145458- Renal cell carcinoma treatment
- Renal and related cancers
- Cancer Genomics and Diagnostics
- Advanced Proteomics Techniques and Applications
- Ferroptosis and cancer prognosis
- Clinical Laboratory Practices and Quality Control
- Cancer Treatment and Pharmacology
- SARS-CoV-2 and COVID-19 Research
- Acute Myocardial Infarction Research
- SARS-CoV-2 detection and testing
- Peptidase Inhibition and Analysis
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Chemotherapy-induced cardiotoxicity and mitigation
- COVID-19 Clinical Research Studies
- Cancer therapeutics and mechanisms
- Metabolomics and Mass Spectrometry Studies
- Meta-analysis and systematic reviews
- Monoclonal and Polyclonal Antibodies Research
- Transplantation: Methods and Outcomes
- Pesticide Residue Analysis and Safety
- Microtubule and mitosis dynamics
- Coronary Interventions and Diagnostics
- Epigenetics and DNA Methylation
- PARP inhibition in cancer therapy
University of Toronto
2015-2025
Toronto General Hospital
2024-2025
University Health Network
2014-2025
Lunenfeld-Tanenbaum Research Institute
2020-2023
Mount Sinai Hospital
2014-2023
St. Michael's Hospital
2014-2022
Hospital for Sick Children
2019
Princess Margaret Cancer Centre
2009-2010
Ontario Institute for Cancer Research
2009-2010
Cancer Institute (WIA)
2010
B cells are known to contribute the anti-tumor immune response, especially in immunogenic tumors such as melanoma, yet humoral immunity has not been characterized these cancers detail. Here we show comprehensive phenotyping samples of circulating and tumor-resident well serum antibodies melanoma patients. Memory enriched compared blood paired feature distinct antibody repertoires, linked specific isotypes. Tumor-associated undergo clonal expansion, class switch recombination, somatic...
The pre-analytical stability of various biochemical analytes requires careful consideration, as it can lead to the release erroneous laboratory results. There is currently significant variability in literature regarding analytes. aim this study was determine 65 whole blood, serum and plasma using a standardized approach.
Abstract Objectives Careful consideration of the pre-analytical process for urine examination is essential to avoid errors and support accurate results decision-making. Our objective was assess impact various factors on test strip quantitative chemistry results, including stability, tube type, fill volume, centrifugation. Methods Residual random specimens were identified. Stability 10 strips 13 parameters assessed at eight time points (2, 4, 6, 8, 24, 48, 72, 96 h) room temperature (RT) 2–8...
Abstract Background Cancer therapy-related cardiac dysfunction (CTRCD) is an important adverse effect in patients receiving potential cardiotoxic cancer therapies. Interpretation of troponin results can be affected by presence macrotroponin, which complicate CTRCD assessment. We aimed to assess whether macrotroponin detectable women with ERBB2 + breast sequential therapy anthracyclines and trastuzumab. Methods A total 20 serum samples from 12 (median age: 55 years, range: 30–69 years) who...
IgE antibodies directed against cancer antigens have demonstrated potent anti-tumour effects in pre-clinical studies. MOv18 IgE, the first-in-class recognising antigen folate receptor alpha (FRα), showed preliminary signs of efficacy a Phase I trial. Treatment was well tolerated, with most common adverse event being transient urticarial skin reactions. We investigated immunological and allergic response parameters associated reactions IgE-treated patients. Expression target antigen, FRα,...
Urine represents an ideal source of clinically relevant biomarkers as it contains a large number proteins and low molecular weight peptides. The comprehensive characterization the normal urinary proteome peptidome can serve reference for future biomarker discovery. Proteomic peptidomic analysis urine also provide insight into physiology disease pathology, especially urogenital diseases.We developed integrated proteomic analytical protocol in urine. We employed ultrafiltration to separate...
Renal cell carcinoma (RCC) is frequently diagnosed incidentally as an early-stage small renal mass (SRM; pT1a, ≤4 cm). Overtreatment of patients with benign or clinically indolent SRMs increasingly common and has resulted in a recent shift treatment recommendations. There are currently no available biomarkers that can accurately predict clinical behavior. Therefore, we set out to identify early RCC progression. We employed quantitative label-free liquid chromatography coupled tandem...
Abstract Background In North America, both messenger RNA (mRNA) vaccines, Pfizer-BioNTech BNT162b2, and Moderna mRNA-1273, each utilizing a 2-dose regimen, have started to be administered individuals. Methods We evaluated the quantitative serologic antibody response following administration of either single dose or doses an mRNA severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine in cohort 98 participants (88 healthcare workers [HCW] 10 solid organ transplant [SOT]...
Cancer immunotherapy with monoclonal antibodies and chimeric antigen receptor (CAR) T cell therapies can benefit from selection of new targets high levels tumor specificity early assessments efficacy safety to derisk potential therapies. Employing mass spectrometry, bioinformatics, immuno-mass spectrometry CRISPR/Cas9 we identified the target tumor-specific SF-25 antibody. We engineered IgE CAR immunotherapies derived clone evaluated for cancer therapy. as tumor-associated SLC3A2, a surface...
// Ashley Di Meo 1, 2, 3 , Rola Saleeb 2 Samantha J. Wala Heba W. Khella 1 Qiang Ding Haiyan Zhai 4 Krishan Kalra Adriana Krizova Manal Gabril 5 Andrew Evans Fadi Brimo 6 Maria D. Pasic 7 Antonio Finelli 8 Eleftherios P. Diamandis and George M. Yousef Department of Laboratory Medicine, Keenan Research Centre for Biomedical Science, Li Ka Shing Knowledge Institute, St. Michael's Hospital, Toronto, ON, Canada Medicine Pathobiology, University Pathology Mount Sinai BioGenex Laboratories,...
Proteomics is defined as the large-scale study of proteins, particularly their structures and functions. Clinical proteomics aims to apply proteomic discoveries technologies patient care. One workhorses mass spectrometry (MS).9 Over last few years we have witnessed spectacular advances in MS-based proteomics; these now allow almost complete proteome identification complex biological fluids, tissues, cells, etc. a matter hours. Recently, spectrometers become faster more sensitive, they can...
In-depth analysis of the human genome sequence has led to annotation approximately 20,000 protein-coding genes. Although mass spectrometry (MS)-based workflows have made a great headway in achieving near genome-wide coverage, an equivalent complete map proteome remains elusive. Delineating spatial distribution all proteins at organ, tissue, and cellular level can offer insight into health disease represents excellent reference for discovery biomarkers therapeutic targets. Here, we performed...
Monitoring estradiol (E2) is important for determining the onset of pubertal development as well in evaluation girls with precocious puberty. However, E2 measurement remains an analytical challenge children, who have lower circulating levels. We developed and evaluated a simple sensitive LC-MS/MS procedure serum quantification pediatric populations established age- sex-specific reference intervals.
Human kallikrein 10 (hk10), a secreted serine protease, was reported to function as tumor suppressor. hK10 immunoexpression has been demonstrated in lactrotrophs and corticotrophs of the nontumorous human adenohypophysis. In present study, for first time we report various surgically removed corticotroph adenoma subtypes. Specimens were fixed formalin embedded paraffin. Immunostaining performed using streptavidin-biotin-peroxidase complex method with an hK10-specific rabbit polyclonal...
Heart failure remains a major cause of morbidity and mortality despite improvements in treatment. This study aimed to evaluate the Alere N-terminal pro B-type natriuretic peptide (NT-proBNP) immunoassay on Abbott Alinity i platform. The analytical performance including precision, linearity, limit quantitation (LOQ), carryover, dilution-recovery, stability was evaluated. A method comparison between NT-proBNP assay Roche Elecsys proBNP II performed using 70 residual plasma samples. Total...