A5074250650- Vector-borne infectious diseases
- Viral Infections and Vectors
- RNA regulation and disease
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Insect symbiosis and bacterial influences
- CRISPR and Genetic Engineering
- Antibiotic Resistance in Bacteria
- Vector-Borne Animal Diseases
- Insect and Pesticide Research
- Mosquito-borne diseases and control
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- Phagocytosis and Immune Regulation
- Neurobiology and Insect Physiology Research
- Plant Reproductive Biology
- Erythrocyte Function and Pathophysiology
- Genomics and Phylogenetic Studies
- Antimicrobial Peptides and Activities
- Bacterial Infections and Vaccines
- Animal Behavior and Reproduction
- Pneumonia and Respiratory Infections
- Insect Resistance and Genetics
University of California, San Francisco
2021-2024
University of Montana
2023
Stanford University
2015-2020
University of Wisconsin–Madison
2012-2013
Adenosine-to-inosine (A-to-I) RNA editing, catalyzed by ADAR enzymes, is a ubiquitous mechanism that generates transcriptomic diversity. This process particularly important for proper neuronal function; however, little known about how editing dynamically regulated between the many functionally distinct populations of brain. Here, we present spatial map in Drosophila brain and show different possess signatures. After purifying sequencing from genetically marked groups nuclei, identified large...
Adenosine-to-inosine RNA editing is catalyzed by adenosine deaminase acting on (ADAR) enzymes that deaminate to inosine. Although many sites are known, few trans regulators have been identified. We perform BioID followed mass spectrometry identify of ADAR1 and ADAR2 in HeLa M17 neuroblastoma cells. known novel ADAR-interacting proteins. Using ENCODE data, we validate characterize a subset the interactors as global or site-specific regulators. Our set includes all four members...
Borrelia burgdorferi (Bb), the causative agent of Lyme disease, adapts to vastly different environments as it cycles between tick vector and vertebrate host. During a bloodmeal, Bb alters its gene expression prepare for infection; however, full range transcriptional changes that occur over several days inside are technically challenging capture. We developed an experimental approach enrich cells longitudinally define their global transcriptomic landscape nymphal Ixodes scapularis ticks...
Steroid hormones act, through their respective nuclear receptors, to regulate target gene expression. Despite critical role in development, physiology, and disease, however, it is still unclear how these systemic cues are refined into tissue-specific responses. We identified a mutation the evolutionarily conserved DEAD box RNA helicase belle/DDX3 that disrupts subset of responses steroid hormone ecdysone during Drosophila melanogaster metamorphosis. demonstrate belle directly regulates...
Adenosine-to-inosine RNA editing modifies maturing mRNAs through the binding of adenosine deaminase acting on (Adar) proteins to double-stranded structures in a process critical for neuronal function. Editing levels at individual sites span broad range and are mediated by both cis-acting elements (surrounding sequence secondary structure) trans-acting factors. Here, we aim determine roles that factors play regulating levels. Using two closely related Drosophila species, D. melanogaster...
Adenosine-to-inosine RNA editing, catalyzed by adenosine deaminase acting on (ADAR) enzymes, alters sequences from those encoded DNA. These editing events are dynamically regulated, but few trans regulators of ADARs known in vivo. Here, we screen RNA-binding proteins for roles regulation with knockdown experiments the Drosophila brain. We identify zinc-finger protein at 72D (Zn72D) as a regulator levels majority sites Zn72D both regulates ADAR and interacts an RNA-dependent fashion, similar...
A vector's susceptibility and ability to transmit a pathogen-termed vector competency-determines disease outcomes, yet the ecological factors influencing tick competency remain largely unknown. Ixodes pacificus, of Borrelia burgdorferi (Bb) in western U.S., feeds on rodents, birds, lizards. Rodents birds are reservoirs for Bb infect juvenile ticks, while lizards refractory cannot feeding ticks. Additionally, lizard bloodmeal contains borreliacidal properties, clearing previously infected...
Members of the bacterial T 6SS a midase e ffector (Tae) superfamily toxins are delivered between competing bacteria to degrade cell wall peptidoglycan. Although Taes share common substrate, they exhibit distinct antimicrobial potency across different competitor species. To investigate molecular basis governing these differences, we quantitatively defined functional determinants Tae1 from Pseudomonas aeruginosa PAO1 using combination nuclear magnetic resonance and high-throughput in vivo...
Borrelia burgdorferi ( Bb ), the causative agent of Lyme disease, must adapt to vastly different environments as bacterium cycles between tick vector and a vertebrate host. During bloodmeal, migrates from midgut salivary glands changes its gene expression, priming for infection. These tick-specific transmission events are dependent on key transcriptional regulators; however, full range that occur over several days inside technically challenging capture. We developed an experimental approach...
Borrelia burgdorferi ( Bb ), the causative agent of Lyme disease, adapts to vastly different environments as it cycles between tick vector and vertebrate host. During a bloodmeal, alters its gene expression prepare for infection; however, full range transcriptional changes that occur over several days inside are technically challenging capture. We developed an experimental approach enrich cells longitudinally define their global transcriptomic landscape nymphal Ixodes scapularis ticks during...
Abstract Mutagenesis screens are a staple of classical genetics. Chemical-induced mutations, however, often difficult and time-consuming to identify. Here, we report that recombination analysis with pairs dominant visible markers provides rapid reliable strategy map mutations in Drosophila melanogaster. This method requires only two generations total six crosses vials estimate the genetic position responsible lesion high accuracy. can then be reliably used identify mutated gene through...
Innate immunity, the first line of defense against pathogens, relies on efficient elimination invading agents by phagocytes. In co-evolution host and pathogen, pathogens developed mechanisms to dampen evade phagocytic clearance. Here, we report that bacterial can clearance macrophages through mimicry at mammalian anti-phagocytic "don't eat me" signaling axis between CD47 (ligand) SIRPα (receptor). We identified a protein, P66, surface
Abstract Adenosine-to-Inosine RNA editing is catalyzed by ADAR enzymes that deaminate adenosine to inosine. While many sites are known, few trans regulators have been identified. We perform BioID followed mass-spectrometry identify of ADAR1 and ADAR2 in HeLa M17 neuroblastoma cells. known novel ADAR-interacting proteins. Using ENCODE data we validate characterize a subset the interactors as global or site-specific regulators. Our set includes all four members DZF-domain-containing family...
Summary Adenosine-to-inosine (A-to-I) RNA editing, catalyzed by ADAR enzymes, is a ubiquitous mechanism that generates transcriptomic diversity. This process particularly important for proper neuronal function; however, little known about how editing dynamically regulated between the many functionally distinct populations of brain. In this resource paper, we present spatial map in Drosophila brain and show different possess signatures. After purifying sequencing from genetically marked...
Abstract Adenosine-to-inosine RNA editing, catalyzed by ADAR enzymes, alters sequences from those encoded DNA. These editing events are dynamically regulated, but few trans regulators of ADARs known in vivo . Here, we screen binding proteins for roles regulation using knockdown experiments the Drosophila brain. We identify Zinc-Finger Protein at 72D (Zn72D) as a regulator levels majority sites Zn72D both regulates protein and interacts with an RNA-dependent fashion, similar to ADAR, is...
Abstract A vector’s susceptibility and ability to transmit a pathogen— termed vector competency—determines disease outcomes, yet the ecological factors influencing tick competency remain largely unknown. Ixodes pacificus , of Borrelia burgdorferi ( Bb ) in western U.S., feeds on rodents, birds, lizards. While rodents birds are reservoirs for infect juvenile ticks, lizards Bb- refractory. Despite I. feeding range hosts, it is undetermined how larval host bloodmeal identity affects future...
Borrelia burgdorferi ( Bb ), the causative agent of Lyme disease, adapts to vastly different environments as it cycles between tick vector and vertebrate host. During a bloodmeal, alters its gene expression prepare for infection; however, full range transcriptional changes that occur over several days inside are technically challenging capture. We developed an experimental approach enrich cells longitudinally define their global transcriptomic landscape nymphal Ixodes scapularis ticks during...
Abstract Members of the bacterial T 6SS a midase e ffector (Tae) superfamily toxins are delivered between competing bacteria to degrade cell wall peptidoglycan. Although Taes share common substrate, they exhibit distinct antimicrobial potency across different competitor species. To investigate molecular basis governing these differences, we quantitatively defined functional determinants Tae1 from Pseudomonas aeruginosa PAO1 using combination n uclear m agnetic resonance (NMR) and...
ABSTRACT Borrelia burgdorferi ( Bb ), the causative agent of Lyme disease, adapts to vastly different environments as it cycles between tick vector and vertebrate host. During a bloodmeal, alters its gene expression prepare for infection; however, full range transcriptional changes that occur over several days inside are technically challenging capture. We developed an experimental approach enrich cells longitudinally define their global transcriptomic landscape nymphal Ixodes scapularis...
Borrelia burgdorferi (Bb), the causative agent of Lyme disease, must adapt to vastly different environments as bacterium cycles between tick vector and a vertebrate host. During bloodmeal, Bb migrates from midgut salivary glands changes its gene expression, priming for infection. These tick-specific events are dependent on key transcriptional regulators; however, full range that occur inside over course several days during transmission technically difficult capture. We developed an...